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Primary sensory neuronal expression of SLURP-1, an endogenous nicotinic acetylcholine receptor ligand.
Neurosci Res. 2009 Aug; 64(4):403-12.NR

Abstract

Secreted mammalian Ly6/urokinase plasminogen activator receptor-related protein-1 (SLURP-1) is a recently identified, endogenous ligand of the alpha7 subunit of nicotinic acetylcholine receptors. SLURP-1 is also the causative gene for an autosomal recessive palmoplantar keratoderma, Mal de Meleda. Although the function of SLURP-1 in keratinocyte development and differentiation has been extensively studied, little is known about its role in the nervous system. In the present study, we analyzed SLURP-1 expression in the spinal cord of rats, as a number of studies suggest spinal nicotinic acetylcholine receptors are important modulators of pain transmission. We detected intense SLURP-1 immunoreactivity in the dorsal horn of the spinal cord, especially in lamina I and outer II. In dorsal root ganglia, SLURP-1 immunoreactivity was detected in small- to medium-sized neurons, where in situ hybridization also revealed the presence of SLURP-1 mRNA. Fluorescent labeling of SLURP-1 partially overlapped that of calcitonin-gene related peptide (CGRP) or substance P (SP) in both the spinal cord dorsal horn and glabrous skin, and electron microscopic analysis revealed colocalization of SLURP-1 with SP or CGRP, in large synaptic vesicles in terminals within the superficial layer of the spinal cord. Finally, sciatic nerve axotomy reduced levels of SLURP-1 immunoreactivity in parallel with that of SP and CGRP in the ipsilateral superficial dorsal horn. These findings suggest that SLURP-1 is expressed in a subset of primary peptidergic sensory neurons.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Pharmacy, Keio University, 1-5-30, Shibakoen, Minato-ku, Tokyo 105-8512, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19409425

Citation

Moriwaki, Yasuhiro, et al. "Primary Sensory Neuronal Expression of SLURP-1, an Endogenous Nicotinic Acetylcholine Receptor Ligand." Neuroscience Research, vol. 64, no. 4, 2009, pp. 403-12.
Moriwaki Y, Watanabe Y, Shinagawa T, et al. Primary sensory neuronal expression of SLURP-1, an endogenous nicotinic acetylcholine receptor ligand. Neurosci Res. 2009;64(4):403-12.
Moriwaki, Y., Watanabe, Y., Shinagawa, T., Kai, M., Miyazawa, M., Okuda, T., Kawashima, K., Yabashi, A., Waguri, S., & Misawa, H. (2009). Primary sensory neuronal expression of SLURP-1, an endogenous nicotinic acetylcholine receptor ligand. Neuroscience Research, 64(4), 403-12. https://doi.org/10.1016/j.neures.2009.04.014
Moriwaki Y, et al. Primary Sensory Neuronal Expression of SLURP-1, an Endogenous Nicotinic Acetylcholine Receptor Ligand. Neurosci Res. 2009;64(4):403-12. PubMed PMID: 19409425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Primary sensory neuronal expression of SLURP-1, an endogenous nicotinic acetylcholine receptor ligand. AU - Moriwaki,Yasuhiro, AU - Watanabe,Yosuke, AU - Shinagawa,Tomoe, AU - Kai,Miho, AU - Miyazawa,Mai, AU - Okuda,Takashi, AU - Kawashima,Koichiro, AU - Yabashi,Atsuko, AU - Waguri,Satoshi, AU - Misawa,Hidemi, Y1 - 2009/05/03/ PY - 2009/03/04/received PY - 2009/04/20/revised PY - 2009/04/21/accepted PY - 2009/5/5/entrez PY - 2009/5/5/pubmed PY - 2009/12/16/medline SP - 403 EP - 12 JF - Neuroscience research JO - Neurosci. Res. VL - 64 IS - 4 N2 - Secreted mammalian Ly6/urokinase plasminogen activator receptor-related protein-1 (SLURP-1) is a recently identified, endogenous ligand of the alpha7 subunit of nicotinic acetylcholine receptors. SLURP-1 is also the causative gene for an autosomal recessive palmoplantar keratoderma, Mal de Meleda. Although the function of SLURP-1 in keratinocyte development and differentiation has been extensively studied, little is known about its role in the nervous system. In the present study, we analyzed SLURP-1 expression in the spinal cord of rats, as a number of studies suggest spinal nicotinic acetylcholine receptors are important modulators of pain transmission. We detected intense SLURP-1 immunoreactivity in the dorsal horn of the spinal cord, especially in lamina I and outer II. In dorsal root ganglia, SLURP-1 immunoreactivity was detected in small- to medium-sized neurons, where in situ hybridization also revealed the presence of SLURP-1 mRNA. Fluorescent labeling of SLURP-1 partially overlapped that of calcitonin-gene related peptide (CGRP) or substance P (SP) in both the spinal cord dorsal horn and glabrous skin, and electron microscopic analysis revealed colocalization of SLURP-1 with SP or CGRP, in large synaptic vesicles in terminals within the superficial layer of the spinal cord. Finally, sciatic nerve axotomy reduced levels of SLURP-1 immunoreactivity in parallel with that of SP and CGRP in the ipsilateral superficial dorsal horn. These findings suggest that SLURP-1 is expressed in a subset of primary peptidergic sensory neurons. SN - 1872-8111 UR - https://www.unboundmedicine.com/medline/citation/19409425/Primary_sensory_neuronal_expression_of_SLURP_1_an_endogenous_nicotinic_acetylcholine_receptor_ligand_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(09)00144-8 DB - PRIME DP - Unbound Medicine ER -