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Biotechnological strategies for phytoremediation of the sulfonated azo dye Direct Red 5B using Blumea malcolmii Hook.
Bioresour Technol. 2009 Sep; 100(18):4104-10.BT

Abstract

Tissue cultured shrub plants of Blumea malcolmii were found to decolorize Malachite green, Red HE8B, Methyl orange, Reactive Red 2 and Direct Red 5B at 20 mg L(-1) concentration to varying extent within three days. A significant induction in the activities of lignin peroxidase, tyrosinase, DCIP (2,6-dichlorophenol-indophenol) reductase, azoreductase and riboflavin reductase in the roots was observed during the decolorization of Direct Red 5B, which indicated their crucial role in the metabolism of the dye. HPLC (High Performance Liquid Chromatography) and FTIR (Fourier Transform Infrared Spectroscopy) analysis of the samples before and after decolorization of the dye confirmed the phytotransformation of Direct Red 5B. The GC-MS (Gas Chromatography Mass Spectroscopy) analysis of the products led us to the identification of three metabolites formed after phytotransformation of the dye as 4-(4-amino-phenylazo)-benzene sulfonic acid, 3-amino-7-carboxyamino-4-hydroxy-naphthalene-2-sulfonic acid and 7-carboxyamino-naphthalene-2-sulfonic acid.

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Authors+Show Affiliations

Kagalkar AN
Department of Biochemistry, Shivaji University, Kolhapur 416 004, India.
Jagtap UB
No affiliation info available
Jadhav JP
No affiliation info available
Bapat VA
No affiliation info available
Govindwar SP
No affiliation info available

MeSH

AsteraceaeAzo CompoundsBiotechnologyBiotransformationChromatography, High Pressure LiquidColorEnvironmental Restoration and RemediationGas Chromatography-Mass SpectrometrySpectroscopy, Fourier Transform Infrared

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19410449

Citation

Kagalkar, Anuradha N., et al. "Biotechnological Strategies for Phytoremediation of the Sulfonated Azo Dye Direct Red 5B Using Blumea Malcolmii Hook." Bioresource Technology, vol. 100, no. 18, 2009, pp. 4104-10.
Kagalkar AN, Jagtap UB, Jadhav JP, et al. Biotechnological strategies for phytoremediation of the sulfonated azo dye Direct Red 5B using Blumea malcolmii Hook. Bioresour Technol. 2009;100(18):4104-10.
Kagalkar, A. N., Jagtap, U. B., Jadhav, J. P., Bapat, V. A., & Govindwar, S. P. (2009). Biotechnological strategies for phytoremediation of the sulfonated azo dye Direct Red 5B using Blumea malcolmii Hook. Bioresource Technology, 100(18), 4104-10. https://doi.org/10.1016/j.biortech.2009.03.049
Kagalkar AN, et al. Biotechnological Strategies for Phytoremediation of the Sulfonated Azo Dye Direct Red 5B Using Blumea Malcolmii Hook. Bioresour Technol. 2009;100(18):4104-10. PubMed PMID: 19410449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biotechnological strategies for phytoremediation of the sulfonated azo dye Direct Red 5B using Blumea malcolmii Hook. AU - Kagalkar,Anuradha N, AU - Jagtap,Umesh B, AU - Jadhav,Jyoti P, AU - Bapat,Vishwas A, AU - Govindwar,Sanjay P, Y1 - 2009/05/01/ PY - 2009/01/24/received PY - 2009/03/15/revised PY - 2009/03/16/accepted PY - 2009/5/5/entrez PY - 2009/5/5/pubmed PY - 2009/8/20/medline SP - 4104 EP - 10 JF - Bioresource technology JO - Bioresour Technol VL - 100 IS - 18 N2 - Tissue cultured shrub plants of Blumea malcolmii were found to decolorize Malachite green, Red HE8B, Methyl orange, Reactive Red 2 and Direct Red 5B at 20 mg L(-1) concentration to varying extent within three days. A significant induction in the activities of lignin peroxidase, tyrosinase, DCIP (2,6-dichlorophenol-indophenol) reductase, azoreductase and riboflavin reductase in the roots was observed during the decolorization of Direct Red 5B, which indicated their crucial role in the metabolism of the dye. HPLC (High Performance Liquid Chromatography) and FTIR (Fourier Transform Infrared Spectroscopy) analysis of the samples before and after decolorization of the dye confirmed the phytotransformation of Direct Red 5B. The GC-MS (Gas Chromatography Mass Spectroscopy) analysis of the products led us to the identification of three metabolites formed after phytotransformation of the dye as 4-(4-amino-phenylazo)-benzene sulfonic acid, 3-amino-7-carboxyamino-4-hydroxy-naphthalene-2-sulfonic acid and 7-carboxyamino-naphthalene-2-sulfonic acid. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/19410449/Biotechnological_strategies_for_phytoremediation_of_the_sulfonated_azo_dye_Direct_Red_5B_using_Blumea_malcolmii_Hook_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(09)00308-3 DB - PRIME DP - Unbound Medicine ER -