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Shiga toxin-producing Escherichia coli and atypical enteropathogenic Escherichia coli strains isolated from healthy sheep of different populations in São Paulo, Brazil.
Lett Appl Microbiol. 2009 Jul; 49(1):53-9.LA

Abstract

AIMS

Sheep are important carriers of Shiga toxin-producing Escherichia coli (STEC) in several countries. However, there are a few reports about ovine STEC in American continent.

METHODS AND RESULTS

About 86 E. coli strains previously isolated from 172 healthy sheep from different farms were studied. PCR was used for detection of stx(1), stx(2), eae, ehxA and saa genes and for the identification of intimin subtypes. Restriction fragment length polymorphism (RFLP)-PCR was performed to investigate the variants of stx(1) and stx(2), and the flagellar antigen (fliC) genes in nonmotile isolates. Five isolates were eae(+) and stx(-), and belonged to serotypes O128:H2/beta-intimin (2), O145:H2/gamma, O153:H7/beta and O178:H7/epsilon. Eighty-one STEC isolates were recovered, and the stx genotypes identified were stx(1c)stx(2d-O118) (46.9%), stx(1c) (27.2%), stx(2d-O118) (23.4%), and stx(1c)stx(2dOX3a) (2.5%). Pulsed-field gel electrophoresis (PFGE) revealed 27 profiles among 53 STEC and atypical enteropathogenic Escherichia coli (EPEC) isolates.

CONCLUSIONS

This study demonstrated that healthy sheep in São Paulo, Brazil, can be carriers of potential human pathogenic STEC and atypical EPEC.

SIGNIFICANCE AND IMPACT OF THE STUDY

As some of the STEC serotypes presently found have been involved with haemolytic uraemic syndrome (HUS) in other countries, the important role of sheep as sources of STEC infection in our settings should not be disregarded.

Authors+Show Affiliations

Departamento de Microbiologia, Instituto de Ciências Biomédicas-II, Universidade de São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19413771

Citation

Vettorato, M P., et al. "Shiga Toxin-producing Escherichia Coli and Atypical Enteropathogenic Escherichia Coli Strains Isolated From Healthy Sheep of Different Populations in São Paulo, Brazil." Letters in Applied Microbiology, vol. 49, no. 1, 2009, pp. 53-9.
Vettorato MP, de Castro AF, Cergole-Novella MC, et al. Shiga toxin-producing Escherichia coli and atypical enteropathogenic Escherichia coli strains isolated from healthy sheep of different populations in São Paulo, Brazil. Lett Appl Microbiol. 2009;49(1):53-9.
Vettorato, M. P., de Castro, A. F., Cergole-Novella, M. C., Camargo, F. L., Irino, K., & Guth, B. E. (2009). Shiga toxin-producing Escherichia coli and atypical enteropathogenic Escherichia coli strains isolated from healthy sheep of different populations in São Paulo, Brazil. Letters in Applied Microbiology, 49(1), 53-9. https://doi.org/10.1111/j.1472-765X.2009.02617.x
Vettorato MP, et al. Shiga Toxin-producing Escherichia Coli and Atypical Enteropathogenic Escherichia Coli Strains Isolated From Healthy Sheep of Different Populations in São Paulo, Brazil. Lett Appl Microbiol. 2009;49(1):53-9. PubMed PMID: 19413771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shiga toxin-producing Escherichia coli and atypical enteropathogenic Escherichia coli strains isolated from healthy sheep of different populations in São Paulo, Brazil. AU - Vettorato,M P, AU - de Castro,A F P, AU - Cergole-Novella,M C, AU - Camargo,F L L, AU - Irino,K, AU - Guth,B E C, Y1 - 2009/04/17/ PY - 2009/5/6/entrez PY - 2009/5/6/pubmed PY - 2009/8/27/medline SP - 53 EP - 9 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 49 IS - 1 N2 - AIMS: Sheep are important carriers of Shiga toxin-producing Escherichia coli (STEC) in several countries. However, there are a few reports about ovine STEC in American continent. METHODS AND RESULTS: About 86 E. coli strains previously isolated from 172 healthy sheep from different farms were studied. PCR was used for detection of stx(1), stx(2), eae, ehxA and saa genes and for the identification of intimin subtypes. Restriction fragment length polymorphism (RFLP)-PCR was performed to investigate the variants of stx(1) and stx(2), and the flagellar antigen (fliC) genes in nonmotile isolates. Five isolates were eae(+) and stx(-), and belonged to serotypes O128:H2/beta-intimin (2), O145:H2/gamma, O153:H7/beta and O178:H7/epsilon. Eighty-one STEC isolates were recovered, and the stx genotypes identified were stx(1c)stx(2d-O118) (46.9%), stx(1c) (27.2%), stx(2d-O118) (23.4%), and stx(1c)stx(2dOX3a) (2.5%). Pulsed-field gel electrophoresis (PFGE) revealed 27 profiles among 53 STEC and atypical enteropathogenic Escherichia coli (EPEC) isolates. CONCLUSIONS: This study demonstrated that healthy sheep in São Paulo, Brazil, can be carriers of potential human pathogenic STEC and atypical EPEC. SIGNIFICANCE AND IMPACT OF THE STUDY: As some of the STEC serotypes presently found have been involved with haemolytic uraemic syndrome (HUS) in other countries, the important role of sheep as sources of STEC infection in our settings should not be disregarded. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/19413771/Shiga_toxin_producing_Escherichia_coli_and_atypical_enteropathogenic_Escherichia_coli_strains_isolated_from_healthy_sheep_of_different_populations_in_São_Paulo_Brazil_ L2 - https://doi.org/10.1111/j.1472-765X.2009.02617.x DB - PRIME DP - Unbound Medicine ER -