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Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report.
Virol J 2009; 6:47VJ

Abstract

BACKGROUND

Orf virus (ORFV), the prototype of the genus Parapoxvirus (PPV), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. Although South American ORFV outbreaks have occurred and diagnosed there are no South American PPV major membrane glycoprotein B2L gene nucleotide sequences available.

CASE PRESENTATION

an outbreak of ovine contagious ecthyma in Midwest Brazil was investigated. The diagnosis was based on clinical examinations and molecular biology techniques. The molecular characterization of the virus was done using PCR amplification, cloning and DNA sequencing of the B2L gene. The phylogenetic analysis demonstrated a high degree of identity with ORFV strains, and the isolate was closest to the ORFV-India 82/04 isolate. Another Brazilian ORFV isolate, NE1, was sequenced for comparative analysis and also showed a high degree of identity with an Asian ORFV strain.

CONCLUSION

Distinct ORFV strains are circulating in Brazil. This is the first report on the phylogenetic analysis of an ORFV in South America.

Authors+Show Affiliations

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. jonatas.abrahao@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19413907

Citation

Abrahão, Jônatas S., et al. "Detection and Phylogenetic Analysis of Orf Virus From Sheep in Brazil: a Case Report." Virology Journal, vol. 6, 2009, p. 47.
Abrahão JS, Campos RK, Trindade GS, et al. Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report. Virol J. 2009;6:47.
Abrahão, J. S., Campos, R. K., Trindade, G. S., Guedes, M. I., Lobato, Z. I., Mazur, C., ... Kroon, E. G. (2009). Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report. Virology Journal, 6, p. 47. doi:10.1186/1743-422X-6-47.
Abrahão JS, et al. Detection and Phylogenetic Analysis of Orf Virus From Sheep in Brazil: a Case Report. Virol J. 2009 May 4;6:47. PubMed PMID: 19413907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and phylogenetic analysis of Orf virus from sheep in Brazil: a case report. AU - Abrahão,Jônatas S, AU - Campos,Rafael K, AU - Trindade,Giliane S, AU - Guedes,Maria I M, AU - Lobato,Zélia I P, AU - Mazur,Carlos, AU - Ferreira,Paulo C P, AU - Bonjardim,Cláudio A, AU - Kroon,Erna G, Y1 - 2009/05/04/ PY - 2009/03/02/received PY - 2009/05/04/accepted PY - 2009/5/6/entrez PY - 2009/5/6/pubmed PY - 2009/6/13/medline SP - 47 EP - 47 JF - Virology journal JO - Virol. J. VL - 6 N2 - BACKGROUND: Orf virus (ORFV), the prototype of the genus Parapoxvirus (PPV), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. Although South American ORFV outbreaks have occurred and diagnosed there are no South American PPV major membrane glycoprotein B2L gene nucleotide sequences available. CASE PRESENTATION: an outbreak of ovine contagious ecthyma in Midwest Brazil was investigated. The diagnosis was based on clinical examinations and molecular biology techniques. The molecular characterization of the virus was done using PCR amplification, cloning and DNA sequencing of the B2L gene. The phylogenetic analysis demonstrated a high degree of identity with ORFV strains, and the isolate was closest to the ORFV-India 82/04 isolate. Another Brazilian ORFV isolate, NE1, was sequenced for comparative analysis and also showed a high degree of identity with an Asian ORFV strain. CONCLUSION: Distinct ORFV strains are circulating in Brazil. This is the first report on the phylogenetic analysis of an ORFV in South America. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/19413907/Detection_and_phylogenetic_analysis_of_Orf_virus_from_sheep_in_Brazil:_a_case_report_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-6-47 DB - PRIME DP - Unbound Medicine ER -