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Increased human telomerase reverse transcriptase (hTERT) mRNA expression but not telomerase activity is related to survival in curatively resected non-small cell lung cancer.
Anticancer Res. 2009 Apr; 29(4):1157-62.AR

Abstract

BACKGROUND

The purpose of this study was to evaluate the significance of human telomerase reverse transcriptase (hTERT) mRNA expression and telomerase activity as prognostic markers in non-small cell lung cancer (NSCLC).

PATIENTS AND METHODS

In a series of 69 curatively resected NSCLC specimens, telomerase activity was analyzed with the telomeric repeat amplification protocol (TRAP) assay and expression of hTERT mRNA by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Partitioning of gene expression levels and protein activities to construct prognostic groups was attempted.

RESULTS

Human hTERT mRNA transcripts were detected in 62 (89.9%) cases of NSCLC. Seven (10.1%) tumors were completely negative for hTERT expression. Dichotomized hTERT levels (<0.42 versus > or =0.42) were associated with prognosis and Kaplan-Meier survival curves demonstrated a significant difference (log rank: p<0.01) with 5-year survival rates of 44.3% (+/-7.1%) for low as compared to 80% (+/-8.9%) for high hTERT mRNA expression. Low hTERT expression was also significantly associated with squamous cell histology (p<0.03). Telomerase activity was not associated with survival, stage, pT and pN categories, histological type or grading. Comparison of hTERT mRNA expression and telomerase activity was possible in 66 patients and showed a significant difference (p<0.0001) by Wilcoxon rank test.

CONCLUSION

This is the first study which demonstrates that high hTERT mRNA expression is associated with improved 5-year survival rates. Expression patterns are distinct among histopathological subtypes of NSCLC and telomerase activity (TRAP) is significantly higher than hTERT mRNA expression.

Authors+Show Affiliations

Department of General, Visceral and Cancer Surgery, University Hospital of Cologne, Joseph-Stelzmann-Strasse 9, D-50931 Cologne, Germany. Ralf.Metzger@uk-koeln.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19414359

Citation

Metzger, Ralf, et al. "Increased Human Telomerase Reverse Transcriptase (hTERT) mRNA Expression but Not Telomerase Activity Is Related to Survival in Curatively Resected Non-small Cell Lung Cancer." Anticancer Research, vol. 29, no. 4, 2009, pp. 1157-62.
Metzger R, Vallbohmer D, Müller-Tidow C, et al. Increased human telomerase reverse transcriptase (hTERT) mRNA expression but not telomerase activity is related to survival in curatively resected non-small cell lung cancer. Anticancer Res. 2009;29(4):1157-62.
Metzger, R., Vallbohmer, D., Müller-Tidow, C., Higashi, H., Bollschweiler, E., Warnecke-Eberz, U., Brabender, J., Baldus, S. E., Xi, H., Berdel, W. E., Serve, H., Hoelscher, A. H., & Schneider, P. M. (2009). Increased human telomerase reverse transcriptase (hTERT) mRNA expression but not telomerase activity is related to survival in curatively resected non-small cell lung cancer. Anticancer Research, 29(4), 1157-62.
Metzger R, et al. Increased Human Telomerase Reverse Transcriptase (hTERT) mRNA Expression but Not Telomerase Activity Is Related to Survival in Curatively Resected Non-small Cell Lung Cancer. Anticancer Res. 2009;29(4):1157-62. PubMed PMID: 19414359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased human telomerase reverse transcriptase (hTERT) mRNA expression but not telomerase activity is related to survival in curatively resected non-small cell lung cancer. AU - Metzger,Ralf, AU - Vallbohmer,Daniel, AU - Müller-Tidow,Carsten, AU - Higashi,Hiroshi, AU - Bollschweiler,Elfriede, AU - Warnecke-Eberz,Ute, AU - Brabender,Jan, AU - Baldus,Stephan E, AU - Xi,Huan, AU - Berdel,Wolfgang E, AU - Serve,Hubert, AU - Hoelscher,Arnulf H, AU - Schneider,Paul M, PY - 2009/5/6/entrez PY - 2009/5/6/pubmed PY - 2009/7/3/medline SP - 1157 EP - 62 JF - Anticancer research JO - Anticancer Res. VL - 29 IS - 4 N2 - BACKGROUND: The purpose of this study was to evaluate the significance of human telomerase reverse transcriptase (hTERT) mRNA expression and telomerase activity as prognostic markers in non-small cell lung cancer (NSCLC). PATIENTS AND METHODS: In a series of 69 curatively resected NSCLC specimens, telomerase activity was analyzed with the telomeric repeat amplification protocol (TRAP) assay and expression of hTERT mRNA by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Partitioning of gene expression levels and protein activities to construct prognostic groups was attempted. RESULTS: Human hTERT mRNA transcripts were detected in 62 (89.9%) cases of NSCLC. Seven (10.1%) tumors were completely negative for hTERT expression. Dichotomized hTERT levels (<0.42 versus > or =0.42) were associated with prognosis and Kaplan-Meier survival curves demonstrated a significant difference (log rank: p<0.01) with 5-year survival rates of 44.3% (+/-7.1%) for low as compared to 80% (+/-8.9%) for high hTERT mRNA expression. Low hTERT expression was also significantly associated with squamous cell histology (p<0.03). Telomerase activity was not associated with survival, stage, pT and pN categories, histological type or grading. Comparison of hTERT mRNA expression and telomerase activity was possible in 66 patients and showed a significant difference (p<0.0001) by Wilcoxon rank test. CONCLUSION: This is the first study which demonstrates that high hTERT mRNA expression is associated with improved 5-year survival rates. Expression patterns are distinct among histopathological subtypes of NSCLC and telomerase activity (TRAP) is significantly higher than hTERT mRNA expression. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/19414359/Increased_human_telomerase_reverse_transcriptase__hTERT__mRNA_expression_but_not_telomerase_activity_is_related_to_survival_in_curatively_resected_non_small_cell_lung_cancer_ L2 - http://ar.iiarjournals.org/cgi/pmidlookup?view=long&amp;pmid=19414359 DB - PRIME DP - Unbound Medicine ER -