Tags

Type your tag names separated by a space and hit enter

Improved localized surface plasmon resonance immunoassay with gold bipyramid substrates.
Anal Chem. 2009 Jun 01; 81(11):4450-5.AC

Abstract

Gold nanoparticles bound to substrates exhibit localized surface plasmon resonance (LSPR) in their optical extinction spectra at visible and near-infrared wavelengths. The LSPR wavelength is sensitive to the surrounding refractive index, enabling a simple, label-free immunoassay when capture antibodies are bound to the nanoparticles. Gold bipyramids are nanoparticles with a penta-twinned crystal structure, which have a sharp LSPR because of their high monodispersity. Bipyramid substrates were found to have a refractive index sensitivity ranging from 288 to 381 nm/RIU (-0.62 to -0.68 eV/RIU), increasing with the nanoparticle size and aspect ratio. In an immunoassay, the bipyramid substrates yielded higher sensitivity than nanorods and nanospheres. An immunoassay sensitivity constant which depends on both the optical properties of the nanoparticle and conjugation chemistry was found to be K(LSPR) = 0.01 nm x microm(2) for gold bipyramids.

Authors+Show Affiliations

Department of Chemistry, Rice University, Houston, Texas 77005-1827, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19415896

Citation

Lee, Seunghyun, et al. "Improved Localized Surface Plasmon Resonance Immunoassay With Gold Bipyramid Substrates." Analytical Chemistry, vol. 81, no. 11, 2009, pp. 4450-5.
Lee S, Mayer KM, Hafner JH. Improved localized surface plasmon resonance immunoassay with gold bipyramid substrates. Anal Chem. 2009;81(11):4450-5.
Lee, S., Mayer, K. M., & Hafner, J. H. (2009). Improved localized surface plasmon resonance immunoassay with gold bipyramid substrates. Analytical Chemistry, 81(11), 4450-5. https://doi.org/10.1021/ac900276n
Lee S, Mayer KM, Hafner JH. Improved Localized Surface Plasmon Resonance Immunoassay With Gold Bipyramid Substrates. Anal Chem. 2009 Jun 1;81(11):4450-5. PubMed PMID: 19415896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved localized surface plasmon resonance immunoassay with gold bipyramid substrates. AU - Lee,Seunghyun, AU - Mayer,Kathryn M, AU - Hafner,Jason H, PY - 2009/5/7/entrez PY - 2009/5/7/pubmed PY - 2009/8/6/medline SP - 4450 EP - 5 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 11 N2 - Gold nanoparticles bound to substrates exhibit localized surface plasmon resonance (LSPR) in their optical extinction spectra at visible and near-infrared wavelengths. The LSPR wavelength is sensitive to the surrounding refractive index, enabling a simple, label-free immunoassay when capture antibodies are bound to the nanoparticles. Gold bipyramids are nanoparticles with a penta-twinned crystal structure, which have a sharp LSPR because of their high monodispersity. Bipyramid substrates were found to have a refractive index sensitivity ranging from 288 to 381 nm/RIU (-0.62 to -0.68 eV/RIU), increasing with the nanoparticle size and aspect ratio. In an immunoassay, the bipyramid substrates yielded higher sensitivity than nanorods and nanospheres. An immunoassay sensitivity constant which depends on both the optical properties of the nanoparticle and conjugation chemistry was found to be K(LSPR) = 0.01 nm x microm(2) for gold bipyramids. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19415896/Improved_localized_surface_plasmon_resonance_immunoassay_with_gold_bipyramid_substrates_ L2 - https://doi.org/10.1021/ac900276n DB - PRIME DP - Unbound Medicine ER -