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Growth parameters of Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and aerobic mesophilic bacteria of apple cider amended with nisin-EDTA.
Foodborne Pathog Dis. 2009 May; 6(4):487-94.FP

Abstract

The effect of nisin (0 or 300 IU/mL), ethylenediamine tetraacetic acid (EDTA, 20 mM), and nisin (300 IU)-EDTA (20 mM) on growth parameters, including lag period (LP) and generation time, of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. in the presence or absence of aerobic mesophilic bacteria of apple cider during storage at 5 degrees C for up to 16 days or 23 degrees C for 16 h was investigated. The growth data were analyzed and fitted to the modified Gompertz model. The LP values for aerobic mesophilic bacteria of apple cider (control) and those amended with EDTA and nisin during storage at 5 degrees C were 1.61, 1.76, and 5.45 days, respectively. In apple cider stored at 23 degrees C for 16 h, the LP values for the same bacteria and treatment were 3.24, 3.56, and 5.85 h, respectively. The LP values for E. coli O157:H7 determined in the presence of aerobic mesophilic bacteria of apple cider stored at 23 degrees C for 16 h was 1.48 h, while populations for L. monocytogenes and Salmonella in the same cider declined. In sterile apple cider left at 23 degrees C for 16 h, the LP values for E. coli O157:H7, Salmonella, and L. monocytogenes averaged 2.74, 2.37, and 3.16 h, respectively. The generation time for these pathogens were 0.402, 0.260, and 0.187 log (CFU/mL)/h, respectively. Addition of nisin and EDTA combination caused a decline in lag phase duration and the populations for all pathogens tested, suggesting possible addition of this additive to freshly prepared apple cider to enhance its microbial safety and prevent costly recalls.

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Authors+Show Affiliations

Ukuku DO
Eastern Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, Wyndmoor, Pennsylvania 19038, USA. dike.ukuku@ars.usda.gov
Zhang H
No affiliation info available
Huang L
No affiliation info available

MeSH

Bacteria, AerobicBeveragesColony Count, MicrobialConsumer Product SafetyDrug SynergismEdetic AcidEscherichia coli O157Food PreservationFood PreservativesKineticsListeria monocytogenesMalusModels, BiologicalNisinSalmonellaTemperatureTime Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19415973

Citation

Ukuku, Dike O., et al. "Growth Parameters of Escherichia Coli O157:H7, Salmonella Spp., Listeria Monocytogenes, and Aerobic Mesophilic Bacteria of Apple Cider Amended With Nisin-EDTA." Foodborne Pathogens and Disease, vol. 6, no. 4, 2009, pp. 487-94.
Ukuku DO, Zhang H, Huang L. Growth parameters of Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and aerobic mesophilic bacteria of apple cider amended with nisin-EDTA. Foodborne Pathog Dis. 2009;6(4):487-94.
Ukuku, D. O., Zhang, H., & Huang, L. (2009). Growth parameters of Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and aerobic mesophilic bacteria of apple cider amended with nisin-EDTA. Foodborne Pathogens and Disease, 6(4), 487-94. https://doi.org/10.1089/fpd.2008.0233
Ukuku DO, Zhang H, Huang L. Growth Parameters of Escherichia Coli O157:H7, Salmonella Spp., Listeria Monocytogenes, and Aerobic Mesophilic Bacteria of Apple Cider Amended With Nisin-EDTA. Foodborne Pathog Dis. 2009;6(4):487-94. PubMed PMID: 19415973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth parameters of Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and aerobic mesophilic bacteria of apple cider amended with nisin-EDTA. AU - Ukuku,Dike O, AU - Zhang,Howard, AU - Huang,Lihan, PY - 2009/5/7/entrez PY - 2009/5/7/pubmed PY - 2009/7/23/medline SP - 487 EP - 94 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 6 IS - 4 N2 - The effect of nisin (0 or 300 IU/mL), ethylenediamine tetraacetic acid (EDTA, 20 mM), and nisin (300 IU)-EDTA (20 mM) on growth parameters, including lag period (LP) and generation time, of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. in the presence or absence of aerobic mesophilic bacteria of apple cider during storage at 5 degrees C for up to 16 days or 23 degrees C for 16 h was investigated. The growth data were analyzed and fitted to the modified Gompertz model. The LP values for aerobic mesophilic bacteria of apple cider (control) and those amended with EDTA and nisin during storage at 5 degrees C were 1.61, 1.76, and 5.45 days, respectively. In apple cider stored at 23 degrees C for 16 h, the LP values for the same bacteria and treatment were 3.24, 3.56, and 5.85 h, respectively. The LP values for E. coli O157:H7 determined in the presence of aerobic mesophilic bacteria of apple cider stored at 23 degrees C for 16 h was 1.48 h, while populations for L. monocytogenes and Salmonella in the same cider declined. In sterile apple cider left at 23 degrees C for 16 h, the LP values for E. coli O157:H7, Salmonella, and L. monocytogenes averaged 2.74, 2.37, and 3.16 h, respectively. The generation time for these pathogens were 0.402, 0.260, and 0.187 log (CFU/mL)/h, respectively. Addition of nisin and EDTA combination caused a decline in lag phase duration and the populations for all pathogens tested, suggesting possible addition of this additive to freshly prepared apple cider to enhance its microbial safety and prevent costly recalls. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/19415973/Growth_parameters_of_Escherichia_coli_O157:H7_Salmonella_spp__Listeria_monocytogenes_and_aerobic_mesophilic_bacteria_of_apple_cider_amended_with_nisin_EDTA_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2008.0233?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -