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l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase.
Br J Dermatol. 2009 Jun; 160(6):1157-62.BJ

Abstract

BACKGROUND

l-Ascorbic acid 2-phosphate (Asc 2-P), a derivative of l-ascorbic acid, promotes elongation of hair shafts in cultured human hair follicles and induces hair growth in mice.

OBJECTIVES

To investigate whether the promotion of hair growth by Asc 2-P is mediated by insulin-like growth factor-1 (IGF-1) and, if so, to investigate the mechanism of the Asc 2-P-induced IGF-1 expression.

METHODS

Dermal papilla (DP) cells were cultured and IGF-1 level was measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay after Asc 2-P treatment in the absence or presence of LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor. Also, hair shaft elongation in cultured human scalp hair follicles and proliferation of cocultured keratinocytes were examined after Asc 2-P treatment in the absence or presence of neutralizing antibody against IGF-1. In addition, keratinocyte proliferation in cultured hair follicles after Asc 2-P treatment in the absence or presence of LY294002 was examined by Ki-67 immunostaining.

RESULTS

IGF-1 mRNA in DP cells was upregulated and IGF-1 protein in the conditioned medium of DP cells was significantly increased after treatment with Asc 2-P. Immunohistochemical staining showed that IGF-1 staining is increased in the DP of cultured human hair follicles by Asc 2-P. The neutralizing antibody against IGF-1 significantly suppressed the Asc 2-P-mediated elongation of hair shafts in hair follicle organ culture and significantly attenuated Asc 2-P-induced growth of cocultured keratinocytes. LY294002 significantly attenuated Asc 2-P-inducible IGF-1 expression and proliferation of follicular keratinocytes in cultured hair follicles.

CONCLUSIONS

These data show that Asc 2-P-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K.

Authors+Show Affiliations

Department of Immunology, Kyungpook National University, Daegu, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19416266

Citation

Kwack, M H., et al. "L-Ascorbic Acid 2-phosphate Promotes Elongation of Hair Shafts Via the Secretion of Insulin-like Growth Factor-1 From Dermal Papilla Cells Through Phosphatidylinositol 3-kinase." The British Journal of Dermatology, vol. 160, no. 6, 2009, pp. 1157-62.
Kwack MH, Shin SH, Kim SR, et al. L-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase. Br J Dermatol. 2009;160(6):1157-62.
Kwack, M. H., Shin, S. H., Kim, S. R., Im, S. U., Han, I. S., Kim, M. K., Kim, J. C., & Sung, Y. K. (2009). L-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase. The British Journal of Dermatology, 160(6), 1157-62. https://doi.org/10.1111/j.1365-2133.2009.09108.x
Kwack MH, et al. L-Ascorbic Acid 2-phosphate Promotes Elongation of Hair Shafts Via the Secretion of Insulin-like Growth Factor-1 From Dermal Papilla Cells Through Phosphatidylinositol 3-kinase. Br J Dermatol. 2009;160(6):1157-62. PubMed PMID: 19416266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase. AU - Kwack,M H, AU - Shin,S H, AU - Kim,S R, AU - Im,S U, AU - Han,I S, AU - Kim,M K, AU - Kim,J C, AU - Sung,Y K, Y1 - 2009/03/26/ PY - 2009/5/7/entrez PY - 2009/5/7/pubmed PY - 2009/11/3/medline SP - 1157 EP - 62 JF - The British journal of dermatology JO - Br J Dermatol VL - 160 IS - 6 N2 - BACKGROUND: l-Ascorbic acid 2-phosphate (Asc 2-P), a derivative of l-ascorbic acid, promotes elongation of hair shafts in cultured human hair follicles and induces hair growth in mice. OBJECTIVES: To investigate whether the promotion of hair growth by Asc 2-P is mediated by insulin-like growth factor-1 (IGF-1) and, if so, to investigate the mechanism of the Asc 2-P-induced IGF-1 expression. METHODS: Dermal papilla (DP) cells were cultured and IGF-1 level was measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay after Asc 2-P treatment in the absence or presence of LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor. Also, hair shaft elongation in cultured human scalp hair follicles and proliferation of cocultured keratinocytes were examined after Asc 2-P treatment in the absence or presence of neutralizing antibody against IGF-1. In addition, keratinocyte proliferation in cultured hair follicles after Asc 2-P treatment in the absence or presence of LY294002 was examined by Ki-67 immunostaining. RESULTS: IGF-1 mRNA in DP cells was upregulated and IGF-1 protein in the conditioned medium of DP cells was significantly increased after treatment with Asc 2-P. Immunohistochemical staining showed that IGF-1 staining is increased in the DP of cultured human hair follicles by Asc 2-P. The neutralizing antibody against IGF-1 significantly suppressed the Asc 2-P-mediated elongation of hair shafts in hair follicle organ culture and significantly attenuated Asc 2-P-induced growth of cocultured keratinocytes. LY294002 significantly attenuated Asc 2-P-inducible IGF-1 expression and proliferation of follicular keratinocytes in cultured hair follicles. CONCLUSIONS: These data show that Asc 2-P-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K. SN - 1365-2133 UR - https://www.unboundmedicine.com/medline/citation/19416266/l_Ascorbic_acid_2_phosphate_promotes_elongation_of_hair_shafts_via_the_secretion_of_insulin_like_growth_factor_1_from_dermal_papilla_cells_through_phosphatidylinositol_3_kinase_ L2 - https://doi.org/10.1111/j.1365-2133.2009.09108.x DB - PRIME DP - Unbound Medicine ER -