TY - JOUR T1 - Effects of a metalloporphyrinic peroxynitrite decomposition catalyst, ww-85, in a mouse model of spinal cord injury. AU - Genovese,Tiziana, AU - Mazzon,Emanuela, AU - Esposito,Emanuela, AU - Di Paola,Rosanna, AU - Murthy,Kanneganti, AU - Neville,Lewis, AU - Bramanti,Placido, AU - Cuzzocrea,Salvatore, PY - 2009/5/7/entrez PY - 2009/5/7/pubmed PY - 2009/8/28/medline SP - 631 EP - 45 JF - Free radical research JO - Free Radic Res VL - 43 IS - 7 N2 - The aim of the present study was to assess the effect of a metalloporphyrinic peroxynitrite decomposition catalyst, ww-85, in the pathophysiology of spinal cord injury (SCI) in mice. Spinal cord trauma was induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy. SCI in mice resulted in severe trauma characterized by oedema, neutrophil infiltration, production of inflammatory mediators, tissue damage and apoptosis. ww-85 treatment (30-300 microg/kg, i.p. 1 h after the SCI) significantly reduced in a dose-dependent manner: (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration (myeloperoxidase activity), (3) nitrotyrosine formation and PARP activation, (4) pro-inflammatory cytokines expression, (5) NF-kappaB activation and (6) apoptosis. Moreover, ww-85 significantly ameliorated the recovery of limb function (evaluated by motor recovery score) in a dose-dependent manner. The results demonstrate that ww-85 treatment reduces the development of inflammation and tissue injury associated with spinal cord trauma. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/19418318/Effects_of_a_metalloporphyrinic_peroxynitrite_decomposition_catalyst_ww_85_in_a_mouse_model_of_spinal_cord_injury_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715760902954126 DB - PRIME DP - Unbound Medicine ER -