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TRPA1-mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1.
Eur J Neurosci. 2009 Apr; 29(8):1568-78.EJ

Abstract

The transient receptor potential (TRP)A1 channel is involved in the transduction of inflammation-induced noxious stimuli from the periphery. Previous studies have characterized the properties of TRPA1 in heterologous expression systems. However, there is little information on the properties of TRPA1-mediated currents in sensory neurons. A capsaicin-sensitive subset of rat and mouse trigeminal ganglion sensory neurons was activated with TRPA1-specific agonists, mustard oil and the cannabinoid WIN55,212. Mustard oil- and WIN55,212-gated currents exhibited marked variability in their kinetics of activation and acute desensitization. TRPA1-mediated responses in neurons also possess a characteristic voltage dependency with profound outward rectification that is influenced by extracellular Ca(2+) and the type and concentration of TRPA1-specific agonists. Examination of TRPA1-mediated responses in TRPA1-containing cells indicated that the features of neuronal TRPA1 are not duplicated in cells expressing only TRPA1 and, instead, can be restored only when TRPA1 and TRPV1 channels are coexpressed. In summary, our results suggest that TRPA1-mediated responses in sensory neurons have distinct characteristics that can be accounted for by the coexpression of the TRPV1 and TRPA1 channels.

Authors+Show Affiliations

Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19419422

Citation

Salas, Margaux M., et al. "TRPA1-mediated Responses in Trigeminal Sensory Neurons: Interaction Between TRPA1 and TRPV1." The European Journal of Neuroscience, vol. 29, no. 8, 2009, pp. 1568-78.
Salas MM, Hargreaves KM, Akopian AN. TRPA1-mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1. Eur J Neurosci. 2009;29(8):1568-78.
Salas, M. M., Hargreaves, K. M., & Akopian, A. N. (2009). TRPA1-mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1. The European Journal of Neuroscience, 29(8), 1568-78. https://doi.org/10.1111/j.1460-9568.2009.06702.x
Salas MM, Hargreaves KM, Akopian AN. TRPA1-mediated Responses in Trigeminal Sensory Neurons: Interaction Between TRPA1 and TRPV1. Eur J Neurosci. 2009;29(8):1568-78. PubMed PMID: 19419422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPA1-mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1. AU - Salas,Margaux M, AU - Hargreaves,Kenneth M, AU - Akopian,Armen N, PY - 2009/5/8/entrez PY - 2009/5/8/pubmed PY - 2009/7/14/medline SP - 1568 EP - 78 JF - The European journal of neuroscience JO - Eur J Neurosci VL - 29 IS - 8 N2 - The transient receptor potential (TRP)A1 channel is involved in the transduction of inflammation-induced noxious stimuli from the periphery. Previous studies have characterized the properties of TRPA1 in heterologous expression systems. However, there is little information on the properties of TRPA1-mediated currents in sensory neurons. A capsaicin-sensitive subset of rat and mouse trigeminal ganglion sensory neurons was activated with TRPA1-specific agonists, mustard oil and the cannabinoid WIN55,212. Mustard oil- and WIN55,212-gated currents exhibited marked variability in their kinetics of activation and acute desensitization. TRPA1-mediated responses in neurons also possess a characteristic voltage dependency with profound outward rectification that is influenced by extracellular Ca(2+) and the type and concentration of TRPA1-specific agonists. Examination of TRPA1-mediated responses in TRPA1-containing cells indicated that the features of neuronal TRPA1 are not duplicated in cells expressing only TRPA1 and, instead, can be restored only when TRPA1 and TRPV1 channels are coexpressed. In summary, our results suggest that TRPA1-mediated responses in sensory neurons have distinct characteristics that can be accounted for by the coexpression of the TRPV1 and TRPA1 channels. SN - 1460-9568 UR - https://www.unboundmedicine.com/medline/citation/19419422/TRPA1_mediated_responses_in_trigeminal_sensory_neurons:_interaction_between_TRPA1_and_TRPV1_ L2 - https://doi.org/10.1111/j.1460-9568.2009.06702.x DB - PRIME DP - Unbound Medicine ER -