Tags

Type your tag names separated by a space and hit enter

Endocannabinoid system in first trimester placenta: low FAAH and high CB1 expression characterize spontaneous miscarriage.

Abstract

Anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were the first endocannabinoids to be characterized, that bind two G protein-coupled receptors, CB1 and CB2. AEA synthesized by multiple pathways, including NAPE-specific phospholipase D (NAPE-PLD) and degraded by the fatty acid amide hydrolase (FAAH). AEA levels are critical in regulating embryo development and the "window" of implantation. We examined the expression of nape-pld mRNA, CB1 and FAAH in human placenta hypothesizing that their altered signaling may contribute to spontaneous miscarriage. First trimester placentas from women with spontaneous miscarriage (group 1) were matched with placentas from women who underwent termination (group 2). Nape-pld expression was analyzed by RT-PCR; CB1 and FAAH expression by Western blot and immunohistochemistry. Nape-pld mRNA expression was higher in group 2 than in group 1. Western blot analysis revealed higher CB1 expression and lower or absent FAAH in group 1 than in group 2. Immunohistochemistry confirmed CB1 and FAAH signals in group 1 and group 2 placentas, respectively. Human placenta contains the enzymes to synthesize AEA. Moreover, placental tissue represents a target for endocannabinoids whose activity may regulate pregnancy outcome. In particular, very low or absent FAAH and high CB1 levels correspond with spontaneous miscarriage.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Dipartimento di Scienze Ginecologiche, Ostetriche e della Riproduzione, II Università degli Studi di Napoli, Largo Madonna delle Grazie 1, 80138 Naples, Italy.

    , , , , , , , , , ,

    Source

    Placenta 30:6 2009 Jun pg 516-22

    MeSH

    Abortion, Induced
    Abortion, Spontaneous
    Adult
    Amidohydrolases
    Animals
    Cannabinoid Receptor Modulators
    Case-Control Studies
    Endocannabinoids
    Female
    Humans
    Male
    Mice
    Phospholipase D
    Placenta
    Pregnancy
    Pregnancy Trimester, First
    Receptor, Cannabinoid, CB1
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19419760

    Citation

    Trabucco, E, et al. "Endocannabinoid System in First Trimester Placenta: Low FAAH and High CB1 Expression Characterize Spontaneous Miscarriage." Placenta, vol. 30, no. 6, 2009, pp. 516-22.
    Trabucco E, Acone G, Marenna A, et al. Endocannabinoid system in first trimester placenta: low FAAH and high CB1 expression characterize spontaneous miscarriage. Placenta. 2009;30(6):516-22.
    Trabucco, E., Acone, G., Marenna, A., Pierantoni, R., Cacciola, G., Chioccarelli, T., ... Cobellis, L. (2009). Endocannabinoid system in first trimester placenta: low FAAH and high CB1 expression characterize spontaneous miscarriage. Placenta, 30(6), pp. 516-22. doi:10.1016/j.placenta.2009.03.015.
    Trabucco E, et al. Endocannabinoid System in First Trimester Placenta: Low FAAH and High CB1 Expression Characterize Spontaneous Miscarriage. Placenta. 2009;30(6):516-22. PubMed PMID: 19419760.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Endocannabinoid system in first trimester placenta: low FAAH and high CB1 expression characterize spontaneous miscarriage. AU - Trabucco,E, AU - Acone,G, AU - Marenna,A, AU - Pierantoni,R, AU - Cacciola,G, AU - Chioccarelli,T, AU - Mackie,K, AU - Fasano,S, AU - Colacurci,N, AU - Meccariello,R, AU - Cobellis,G, AU - Cobellis,L, Y1 - 2009/05/05/ PY - 2009/01/12/received PY - 2009/03/27/revised PY - 2009/03/30/accepted PY - 2009/5/8/entrez PY - 2009/5/8/pubmed PY - 2009/10/1/medline SP - 516 EP - 22 JF - Placenta JO - Placenta VL - 30 IS - 6 N2 - Anandamide (AEA) and 2-arachidonoylglycerol (2-AG) were the first endocannabinoids to be characterized, that bind two G protein-coupled receptors, CB1 and CB2. AEA synthesized by multiple pathways, including NAPE-specific phospholipase D (NAPE-PLD) and degraded by the fatty acid amide hydrolase (FAAH). AEA levels are critical in regulating embryo development and the "window" of implantation. We examined the expression of nape-pld mRNA, CB1 and FAAH in human placenta hypothesizing that their altered signaling may contribute to spontaneous miscarriage. First trimester placentas from women with spontaneous miscarriage (group 1) were matched with placentas from women who underwent termination (group 2). Nape-pld expression was analyzed by RT-PCR; CB1 and FAAH expression by Western blot and immunohistochemistry. Nape-pld mRNA expression was higher in group 2 than in group 1. Western blot analysis revealed higher CB1 expression and lower or absent FAAH in group 1 than in group 2. Immunohistochemistry confirmed CB1 and FAAH signals in group 1 and group 2 placentas, respectively. Human placenta contains the enzymes to synthesize AEA. Moreover, placental tissue represents a target for endocannabinoids whose activity may regulate pregnancy outcome. In particular, very low or absent FAAH and high CB1 levels correspond with spontaneous miscarriage. SN - 1532-3102 UR - https://www.unboundmedicine.com/medline/citation/19419760/Endocannabinoid_system_in_first_trimester_placenta:_low_FAAH_and_high_CB1_expression_characterize_spontaneous_miscarriage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(09)00110-6 DB - PRIME DP - Unbound Medicine ER -