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Atomic force microscopy and surface-enhanced Raman scattering detection of DNA based on DNA-nanoparticle complexes.
Nanotechnology. 2009 Mar 25; 20(12):125502.N

Abstract

We report a simple method for the label-free detection of double-stranded DNA using surface-enhanced Raman scattering (SERS). We prepared cetyltrimethylammonium bromide (CTAB)-capped silver nanoparticles and a DNA-nanoparticle complex by adding silver nanoparticles to lambda-DNA solutions. In the present study, the utilization of CTAB-capped silver nanoparticles facilitates the electrostatic interaction between DNA molecules and silver nanoparticles; at the same time, the introduction of DNA avoids adding aggregating agent for the formation of nanoparticle aggregates to obtain large enhancement of DNA, because the DNA acts as both the probe molecules and aggregating agent of Ag nanoparticles. Atomic force microscopy (AFM) studies show that the morphology of DNA-Ag nanoparticle complexes seems to be determined by the concentrations of the DNA and the nanoparticles. Surface-enhanced Raman scattering (SERS) studies show that the morphology of the complexes plays a significant role in the intensity of SERS signals of DNA, and the best signal enhancement of DNA can be obtained by fine-tuning the experimental parameters. The SERS spectrum affords important structural information about the bases, phosphate backbone, and the conformation of DNA after mixing the DNA solutions with the Ag sol.

Authors+Show Affiliations

State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate school of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19420468

Citation

Sun, Lanlan, et al. "Atomic Force Microscopy and Surface-enhanced Raman Scattering Detection of DNA Based On DNA-nanoparticle Complexes." Nanotechnology, vol. 20, no. 12, 2009, p. 125502.
Sun L, Sun Y, Xu F, et al. Atomic force microscopy and surface-enhanced Raman scattering detection of DNA based on DNA-nanoparticle complexes. Nanotechnology. 2009;20(12):125502.
Sun, L., Sun, Y., Xu, F., Zhang, Y., Yang, T., Guo, C., Liu, Z., & Li, Z. (2009). Atomic force microscopy and surface-enhanced Raman scattering detection of DNA based on DNA-nanoparticle complexes. Nanotechnology, 20(12), 125502. https://doi.org/10.1088/0957-4484/20/12/125502
Sun L, et al. Atomic Force Microscopy and Surface-enhanced Raman Scattering Detection of DNA Based On DNA-nanoparticle Complexes. Nanotechnology. 2009 Mar 25;20(12):125502. PubMed PMID: 19420468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Atomic force microscopy and surface-enhanced Raman scattering detection of DNA based on DNA-nanoparticle complexes. AU - Sun,Lanlan, AU - Sun,Yujing, AU - Xu,Fugang, AU - Zhang,Yue, AU - Yang,Tao, AU - Guo,Cunlan, AU - Liu,Zhelin, AU - Li,Zhuang, Y1 - 2009/03/03/ PY - 2009/5/8/entrez PY - 2009/5/8/pubmed PY - 2009/8/25/medline SP - 125502 EP - 125502 JF - Nanotechnology JO - Nanotechnology VL - 20 IS - 12 N2 - We report a simple method for the label-free detection of double-stranded DNA using surface-enhanced Raman scattering (SERS). We prepared cetyltrimethylammonium bromide (CTAB)-capped silver nanoparticles and a DNA-nanoparticle complex by adding silver nanoparticles to lambda-DNA solutions. In the present study, the utilization of CTAB-capped silver nanoparticles facilitates the electrostatic interaction between DNA molecules and silver nanoparticles; at the same time, the introduction of DNA avoids adding aggregating agent for the formation of nanoparticle aggregates to obtain large enhancement of DNA, because the DNA acts as both the probe molecules and aggregating agent of Ag nanoparticles. Atomic force microscopy (AFM) studies show that the morphology of DNA-Ag nanoparticle complexes seems to be determined by the concentrations of the DNA and the nanoparticles. Surface-enhanced Raman scattering (SERS) studies show that the morphology of the complexes plays a significant role in the intensity of SERS signals of DNA, and the best signal enhancement of DNA can be obtained by fine-tuning the experimental parameters. The SERS spectrum affords important structural information about the bases, phosphate backbone, and the conformation of DNA after mixing the DNA solutions with the Ag sol. SN - 1361-6528 UR - https://www.unboundmedicine.com/medline/citation/19420468/Atomic_force_microscopy_and_surface_enhanced_Raman_scattering_detection_of_DNA_based_on_DNA_nanoparticle_complexes_ L2 - https://doi.org/10.1088/0957-4484/20/12/125502 DB - PRIME DP - Unbound Medicine ER -