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Na(+) but not Cl(-) or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings.
J Plant Physiol. 2009 Oct 15; 166(15):1598-606.JP

Abstract

Glutathione reductase (GR, EC 1,6.4.2) is an important reactive oxygen species-scavenging enzyme. The present study examined the relative importance of Na(+), Cl(-), and the osmotic component in NaCl-induced expression of Oryza sativa glutathione reductase (OsGR) genes in rice roots. Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome. The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl. Treatment with 150 mM NaCl and 150 mM NaNO(3) affected OsGR2 and OsGR3 induction similarly, which suggests that Na(+) but not Cl(-) is required for the NaCl-induced expression of OsGR2 and OsGR3. We also show that Na(+) but not Cl(-) is required for NaCl-enhanced GR activity and hydrogen peroxide (H(2)O(2)) production in rice roots. In addition to its component of ion toxicity, salt concentration in soil results in an osmotic effect. Here, we show that OsGR2 and OsGR3 expression, GR activity, and H(2)O(2) content were not affected at a concentration of mannitol iso-osmotic with 150 mM NaCl. NaCl-induced OsGR2 and OsGR3 in rice roots could be associated with Na(+) but not an osmotic component.

Authors+Show Affiliations

Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19423186

Citation

Hong, Chwan-Yang, et al. "Na(+) but Not Cl(-) or Osmotic Stress Is Involved in NaCl-induced Expression of Glutathione Reductase in Roots of Rice Seedlings." Journal of Plant Physiology, vol. 166, no. 15, 2009, pp. 1598-606.
Hong CY, Chao YY, Yang MY, et al. Na(+) but not Cl(-) or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings. J Plant Physiol. 2009;166(15):1598-606.
Hong, C. Y., Chao, Y. Y., Yang, M. Y., Cho, S. C., & Huei Kao, C. (2009). Na(+) but not Cl(-) or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings. Journal of Plant Physiology, 166(15), 1598-606. https://doi.org/10.1016/j.jplph.2009.04.001
Hong CY, et al. Na(+) but Not Cl(-) or Osmotic Stress Is Involved in NaCl-induced Expression of Glutathione Reductase in Roots of Rice Seedlings. J Plant Physiol. 2009 Oct 15;166(15):1598-606. PubMed PMID: 19423186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Na(+) but not Cl(-) or osmotic stress is involved in NaCl-induced expression of Glutathione reductase in roots of rice seedlings. AU - Hong,Chwan-Yang, AU - Chao,Yun-Yang, AU - Yang,Min-Yu, AU - Cho,Shih-Chueh, AU - Huei Kao,Ching, Y1 - 2009/05/07/ PY - 2009/02/24/received PY - 2009/04/01/revised PY - 2009/04/01/accepted PY - 2009/5/9/entrez PY - 2009/5/9/pubmed PY - 2009/11/18/medline SP - 1598 EP - 606 JF - Journal of plant physiology JO - J Plant Physiol VL - 166 IS - 15 N2 - Glutathione reductase (GR, EC 1,6.4.2) is an important reactive oxygen species-scavenging enzyme. The present study examined the relative importance of Na(+), Cl(-), and the osmotic component in NaCl-induced expression of Oryza sativa glutathione reductase (OsGR) genes in rice roots. Semi-quantitative RT-PCR was used to quantify the mRNA levels for one cytosolic (OsGR2) and two chloroplastic (OsGR1 and OsGR3) isoforms of GR identified in the rice genome. The expression of OsGR2 and OsGR3 but not OsGR1 was increased in rice roots treated with NaCl. Treatment with 150 mM NaCl and 150 mM NaNO(3) affected OsGR2 and OsGR3 induction similarly, which suggests that Na(+) but not Cl(-) is required for the NaCl-induced expression of OsGR2 and OsGR3. We also show that Na(+) but not Cl(-) is required for NaCl-enhanced GR activity and hydrogen peroxide (H(2)O(2)) production in rice roots. In addition to its component of ion toxicity, salt concentration in soil results in an osmotic effect. Here, we show that OsGR2 and OsGR3 expression, GR activity, and H(2)O(2) content were not affected at a concentration of mannitol iso-osmotic with 150 mM NaCl. NaCl-induced OsGR2 and OsGR3 in rice roots could be associated with Na(+) but not an osmotic component. SN - 1618-1328 UR - https://www.unboundmedicine.com/medline/citation/19423186/Na_+__but_not_Cl____or_osmotic_stress_is_involved_in_NaCl_induced_expression_of_Glutathione_reductase_in_roots_of_rice_seedlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0176-1617(09)00133-3 DB - PRIME DP - Unbound Medicine ER -