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CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines.
Oncol Rep. 2009 Jun; 21(6):1413-9.OR

Abstract

CKD-602 7-[2-(N-isopropylamino)ethyl]-(20S)-camptothecin, belotecan) is a synthetic water-soluble camptothecin derivative and topoisomerase inhibitor that has been shown to have clinical anticancer effect against ovarian and lung cancer. We studied its anticancer effects on four human glioma cell lines, U87 MG, U343 MG, U251 MG and LN229. Cell viability was quantified by a modified 2-(2-methoxy-4-nitropheyl)-3-(4-nitropheyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt and significant time- and dose-dependent cytotoxicity was observed in all cell lines. Susceptibility to CKD-602 at 48 h after treatment varied among the four cell lines and their IC50 value was as follows: 9.07 nM (95% CI 0.18-37.42) for LN229, 14.57 nM (95% CI 0.86-47.33) for U251 MG, 29.13 nM (95% CI 0.35-101.23) for U343 MG, and 84.66 nM (95% CI 34.63-148.25) for U87 MG. CKD-602 induced cell cycle arrest at G2 phase and produced antiproliferative activity and apoptosis in all cell lines. Thus, CKD-602 showed a significant anticancer effect on glioma cells in vitro and is a promising candidate for further studies on malignant gliomas.

Authors+Show Affiliations

Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19424618

Citation

Kim, Young-Yim, et al. "CKD-602, a Camptothecin Derivative, Inhibits Proliferation and Induces Apoptosis in Glioma Cell Lines." Oncology Reports, vol. 21, no. 6, 2009, pp. 1413-9.
Kim YY, Park CK, Kim SK, et al. CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines. Oncol Rep. 2009;21(6):1413-9.
Kim, Y. Y., Park, C. K., Kim, S. K., Phi, J. H., Kim, J. H., Kim, C. Y., Wang, K. C., & Cho, B. K. (2009). CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines. Oncology Reports, 21(6), 1413-9.
Kim YY, et al. CKD-602, a Camptothecin Derivative, Inhibits Proliferation and Induces Apoptosis in Glioma Cell Lines. Oncol Rep. 2009;21(6):1413-9. PubMed PMID: 19424618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines. AU - Kim,Young-Yim, AU - Park,Chul-Kee, AU - Kim,Seung-Ki, AU - Phi,Ji-Hoon, AU - Kim,Jin-Hyun, AU - Kim,Chae-Yong, AU - Wang,Kyu-Chang, AU - Cho,Byung-Kyu, PY - 2009/5/9/entrez PY - 2009/5/9/pubmed PY - 2009/7/10/medline SP - 1413 EP - 9 JF - Oncology reports JO - Oncol Rep VL - 21 IS - 6 N2 - CKD-602 7-[2-(N-isopropylamino)ethyl]-(20S)-camptothecin, belotecan) is a synthetic water-soluble camptothecin derivative and topoisomerase inhibitor that has been shown to have clinical anticancer effect against ovarian and lung cancer. We studied its anticancer effects on four human glioma cell lines, U87 MG, U343 MG, U251 MG and LN229. Cell viability was quantified by a modified 2-(2-methoxy-4-nitropheyl)-3-(4-nitropheyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt and significant time- and dose-dependent cytotoxicity was observed in all cell lines. Susceptibility to CKD-602 at 48 h after treatment varied among the four cell lines and their IC50 value was as follows: 9.07 nM (95% CI 0.18-37.42) for LN229, 14.57 nM (95% CI 0.86-47.33) for U251 MG, 29.13 nM (95% CI 0.35-101.23) for U343 MG, and 84.66 nM (95% CI 34.63-148.25) for U87 MG. CKD-602 induced cell cycle arrest at G2 phase and produced antiproliferative activity and apoptosis in all cell lines. Thus, CKD-602 showed a significant anticancer effect on glioma cells in vitro and is a promising candidate for further studies on malignant gliomas. SN - 1021-335X UR - https://www.unboundmedicine.com/medline/citation/19424618/CKD_602_a_camptothecin_derivative_inhibits_proliferation_and_induces_apoptosis_in_glioma_cell_lines_ L2 - http://www.spandidos-publications.com/or/21/6/1413 DB - PRIME DP - Unbound Medicine ER -