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Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities.
Food Chem Toxicol. 2009 Jun; 47(6):1161-7.FC

Abstract

In order to determine antioxidant activity, the five extracts/fractions of red onion peel were studied for their total content of phenolics (TPC), flavonoids (TFC), antioxidant activity (AOA), free radical scavenging activity (FRSA), assayed by DPPH radical in the terms of anti-radical power (ARP) and reducing power (RP), expressed as ascorbic acid equivalents (ASE)/ml. High TPC (384.7 +/- 5.0 mg GAE/g), TFC (165.2+/- 3.2 mg QE/g), AOA (97.4 +/- 7.6%), ARP (75.3 +/-4.5) and RP (1.6 +/-0.3 ASE/ml) were found for the ethyl acetate (EA) fraction. EA fraction had markedly higher antioxidant capacity than butylated hydroxytoluene (BHT) in preventive or scavenging capacities against FeCl3-induced lipid peroxidation, protein fragmentation, hydroxyl (site-specific and non-site-specific), superoxide anion and nitric oxide radicals. EA fraction also showed dose dependent antimutagenic activity by following the inhibition of tobacco-induced mutagenicity in Salmonella typhimurium strains (TA102) and hydroxyl radical-induced nicking in plasmid pUC18 DNA. HPLC and MS/MS analysis showed the presence of ferulic, gallic, protocatechuic acids, quercetin and kaempferol. The large amount of polyphenols contained in EA fraction may cause its strong antioxidant and antimutagenic properties. This information shows that EA fraction of red onion peel can be used as natural antioxidant in nutraceutical preparations.

Authors+Show Affiliations

Nutraceutical Chemistry, National Botanical Research Institute, Lucknow 226 001, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19425188

Citation

Singh, Brahma N., et al. "Polyphenolics From Various Extracts/fractions of Red Onion (Allium Cepa) Peel With Potent Antioxidant and Antimutagenic Activities." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 47, no. 6, 2009, pp. 1161-7.
Singh BN, Singh BR, Singh RL, et al. Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food Chem Toxicol. 2009;47(6):1161-7.
Singh, B. N., Singh, B. R., Singh, R. L., Prakash, D., Singh, D. P., Sarma, B. K., Upadhyay, G., & Singh, H. B. (2009). Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 47(6), 1161-7.
Singh BN, et al. Polyphenolics From Various Extracts/fractions of Red Onion (Allium Cepa) Peel With Potent Antioxidant and Antimutagenic Activities. Food Chem Toxicol. 2009;47(6):1161-7. PubMed PMID: 19425188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. AU - Singh,Brahma N, AU - Singh,B R, AU - Singh,R L, AU - Prakash,D, AU - Singh,D P, AU - Sarma,B K, AU - Upadhyay,G, AU - Singh,H B, PY - 2009/5/9/entrez PY - 2009/5/9/pubmed PY - 2009/7/17/medline SP - 1161 EP - 7 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 47 IS - 6 N2 - In order to determine antioxidant activity, the five extracts/fractions of red onion peel were studied for their total content of phenolics (TPC), flavonoids (TFC), antioxidant activity (AOA), free radical scavenging activity (FRSA), assayed by DPPH radical in the terms of anti-radical power (ARP) and reducing power (RP), expressed as ascorbic acid equivalents (ASE)/ml. High TPC (384.7 +/- 5.0 mg GAE/g), TFC (165.2+/- 3.2 mg QE/g), AOA (97.4 +/- 7.6%), ARP (75.3 +/-4.5) and RP (1.6 +/-0.3 ASE/ml) were found for the ethyl acetate (EA) fraction. EA fraction had markedly higher antioxidant capacity than butylated hydroxytoluene (BHT) in preventive or scavenging capacities against FeCl3-induced lipid peroxidation, protein fragmentation, hydroxyl (site-specific and non-site-specific), superoxide anion and nitric oxide radicals. EA fraction also showed dose dependent antimutagenic activity by following the inhibition of tobacco-induced mutagenicity in Salmonella typhimurium strains (TA102) and hydroxyl radical-induced nicking in plasmid pUC18 DNA. HPLC and MS/MS analysis showed the presence of ferulic, gallic, protocatechuic acids, quercetin and kaempferol. The large amount of polyphenols contained in EA fraction may cause its strong antioxidant and antimutagenic properties. This information shows that EA fraction of red onion peel can be used as natural antioxidant in nutraceutical preparations. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19425188/Polyphenolics_from_various_extracts/fractions_of_red_onion__Allium_cepa__peel_with_potent_antioxidant_and_antimutagenic_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(09)00070-2 DB - PRIME DP - Unbound Medicine ER -