Tags

Type your tag names separated by a space and hit enter

Suppressive effects of swainsonine on C6 glioma cell in vitro and in vivo.
Phytomedicine. 2009 Nov; 16(11):1070-4.P

Abstract

Swainsonine, an extract from Astragalus membranaceus, is known for its anti-cancer effects and could prevent metastases. In order to investigate the effects and mechanisms of swainsonine in C6 glioma cells, we carry out correlated experiments in vitro and in vivo. After treatment with swainsonine, the effective dose and IC(50) value of swainsonine in the C6 glioma cell were examined using the MTT assay. Cell cycle distribution and apoptotic rates were analyzed using FCM and [Ca(2+)](i) was measured by LSCM. Expressions of p16 and p53 protein were evaluated by immunocytochemical methods. Simultaneously, glioma-bearing rats were administered swainsonine at doses of 2, 4 and 8 mg/kg body wt. The inhibition rate was calculated and pathological sections were observed. The results indicated that the growth of C6 glioma cells is inhibited by swainsonine in vitro, with an IC(50) value within 24h of 0.05 microg/ml. Increases in swainsonine correlate with S phase percentages of 11.3%, 11.6% and 12.4%, respectively. Moreover, the expression of apoptosis inhibiting p53 and p16 protein decreases gradually. Tumor weight in vivo decreased clearly and HE dyeing of tumor tissue showed gray, its texture was soft, with necrosis and hemorrhagic concentrated inward. Swainsonine could inhibit the proliferation of C6 glioma cells in vitro and the growth of C6 glioma in vivo. The mechanisms of swainsonine-induced apoptosis may relate with the expression of apoptosis-related genes and overloading-[Ca(2+)](i)-induced endoplasmic reticulum stress.

Authors+Show Affiliations

Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19427771

Citation

Sun, Ji-Yuan, et al. "Suppressive Effects of Swainsonine On C6 Glioma Cell in Vitro and in Vivo." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 16, no. 11, 2009, pp. 1070-4.
Sun JY, Yang H, Miao S, et al. Suppressive effects of swainsonine on C6 glioma cell in vitro and in vivo. Phytomedicine. 2009;16(11):1070-4.
Sun, J. Y., Yang, H., Miao, S., Li, J. P., Wang, S. W., Zhu, M. Z., Xie, Y. H., Wang, J. B., Liu, Z., & Yang, Q. (2009). Suppressive effects of swainsonine on C6 glioma cell in vitro and in vivo. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 16(11), 1070-4. https://doi.org/10.1016/j.phymed.2009.02.012
Sun JY, et al. Suppressive Effects of Swainsonine On C6 Glioma Cell in Vitro and in Vivo. Phytomedicine. 2009;16(11):1070-4. PubMed PMID: 19427771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppressive effects of swainsonine on C6 glioma cell in vitro and in vivo. AU - Sun,Ji-Yuan, AU - Yang,Hao, AU - Miao,Shan, AU - Li,Ji-Peng, AU - Wang,Si-Wang, AU - Zhu,Miao-Zhang, AU - Xie,Yan-Hua, AU - Wang,Jian-Bo, AU - Liu,Zhe, AU - Yang,Qian, Y1 - 2009/05/08/ PY - 2009/5/12/entrez PY - 2009/5/12/pubmed PY - 2010/1/28/medline SP - 1070 EP - 4 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 16 IS - 11 N2 - Swainsonine, an extract from Astragalus membranaceus, is known for its anti-cancer effects and could prevent metastases. In order to investigate the effects and mechanisms of swainsonine in C6 glioma cells, we carry out correlated experiments in vitro and in vivo. After treatment with swainsonine, the effective dose and IC(50) value of swainsonine in the C6 glioma cell were examined using the MTT assay. Cell cycle distribution and apoptotic rates were analyzed using FCM and [Ca(2+)](i) was measured by LSCM. Expressions of p16 and p53 protein were evaluated by immunocytochemical methods. Simultaneously, glioma-bearing rats were administered swainsonine at doses of 2, 4 and 8 mg/kg body wt. The inhibition rate was calculated and pathological sections were observed. The results indicated that the growth of C6 glioma cells is inhibited by swainsonine in vitro, with an IC(50) value within 24h of 0.05 microg/ml. Increases in swainsonine correlate with S phase percentages of 11.3%, 11.6% and 12.4%, respectively. Moreover, the expression of apoptosis inhibiting p53 and p16 protein decreases gradually. Tumor weight in vivo decreased clearly and HE dyeing of tumor tissue showed gray, its texture was soft, with necrosis and hemorrhagic concentrated inward. Swainsonine could inhibit the proliferation of C6 glioma cells in vitro and the growth of C6 glioma in vivo. The mechanisms of swainsonine-induced apoptosis may relate with the expression of apoptosis-related genes and overloading-[Ca(2+)](i)-induced endoplasmic reticulum stress. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/19427771/Suppressive_effects_of_swainsonine_on_C6_glioma_cell_in_vitro_and_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(09)00047-6 DB - PRIME DP - Unbound Medicine ER -