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Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses.
Vaccine. 2009 May 18; 27(23):3083-9.V

Abstract

The combination of rapid evolution and high mortality in human cases of infections has raised concerns that the H5N1 avian influenza virus may become a new, possibly severe, pandemic virus. Vaccination is likely to be the most efficient strategy to mitigate the impact of the next influenza pandemic. The present study evaluates B and T cell immune responses generated by the H5N1 viral antigens, hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), or the M2 ion channel in parallel, expressed from a DNA vaccine vehicle. Protection studies of immunized mice challenged with 100 LD50 of homologous or heterologous H5N1 viruses indicate that HA afforded better protection than the NA, NP or M2 DNA vaccines. The antibody response was also higher in HA-vaccinated mice as determined by hemagglutination inhibition (HI) and neutralizing antibodies (NAB) assays. Interestingly, the T cell response was higher against HA than against NA, NP or M2 and was detectable at low doses of the DNA-HA vaccine capable of inducing complete protection, despite the absence of a detectable B cell response. This study emphasizes the need to evaluate the relationship between both arms of the adaptive immune responses in regards to protective efficacy against influenza virus.

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Authors+Show Affiliations

Patel A
Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Tran K
No affiliation info available
Gray M
No affiliation info available
Li Y
No affiliation info available
Ao Z
No affiliation info available
Yao X
No affiliation info available
Kobasa D
No affiliation info available
Kobinger GP
No affiliation info available

MeSH

Amino Acid SequenceAnimalsAntibody FormationAntigens, ViralCell LineConserved SequenceFemaleHemagglutinin Glycoproteins, Influenza VirusHumansInfluenza A Virus, H5N1 SubtypeInfluenza, HumanLymphocyte ActivationMacaca fascicularisMaleMiceMice, Inbred BALB CNeuraminidaseNucleoproteinsVaccinationVaccines, DNAViral Matrix Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19428922

Citation

Patel, Ami, et al. "Evaluation of Conserved and Variable Influenza Antigens for Immunization Against Different Isolates of H5N1 Viruses." Vaccine, vol. 27, no. 23, 2009, pp. 3083-9.
Patel A, Tran K, Gray M, et al. Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses. Vaccine. 2009;27(23):3083-9.
Patel, A., Tran, K., Gray, M., Li, Y., Ao, Z., Yao, X., Kobasa, D., & Kobinger, G. P. (2009). Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses. Vaccine, 27(23), 3083-9. https://doi.org/10.1016/j.vaccine.2009.03.023
Patel A, et al. Evaluation of Conserved and Variable Influenza Antigens for Immunization Against Different Isolates of H5N1 Viruses. Vaccine. 2009 May 18;27(23):3083-9. PubMed PMID: 19428922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses. AU - Patel,Ami, AU - Tran,Kaylie, AU - Gray,Michael, AU - Li,Yan, AU - Ao,Zhujun, AU - Yao,Xiaojian, AU - Kobasa,Darwyn, AU - Kobinger,Gary P, Y1 - 2009/04/01/ PY - 2008/11/18/received PY - 2009/03/02/revised PY - 2009/03/09/accepted PY - 2009/5/12/entrez PY - 2009/5/12/pubmed PY - 2009/7/1/medline SP - 3083 EP - 9 JF - Vaccine JO - Vaccine VL - 27 IS - 23 N2 - The combination of rapid evolution and high mortality in human cases of infections has raised concerns that the H5N1 avian influenza virus may become a new, possibly severe, pandemic virus. Vaccination is likely to be the most efficient strategy to mitigate the impact of the next influenza pandemic. The present study evaluates B and T cell immune responses generated by the H5N1 viral antigens, hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), or the M2 ion channel in parallel, expressed from a DNA vaccine vehicle. Protection studies of immunized mice challenged with 100 LD50 of homologous or heterologous H5N1 viruses indicate that HA afforded better protection than the NA, NP or M2 DNA vaccines. The antibody response was also higher in HA-vaccinated mice as determined by hemagglutination inhibition (HI) and neutralizing antibodies (NAB) assays. Interestingly, the T cell response was higher against HA than against NA, NP or M2 and was detectable at low doses of the DNA-HA vaccine capable of inducing complete protection, despite the absence of a detectable B cell response. This study emphasizes the need to evaluate the relationship between both arms of the adaptive immune responses in regards to protective efficacy against influenza virus. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/19428922/Evaluation_of_conserved_and_variable_influenza_antigens_for_immunization_against_different_isolates_of_H5N1_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(09)00406-X DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓16]

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