Tags

Type your tag names separated by a space and hit enter

Chrysanthemum indicum Linné extract inhibits the inflammatory response by suppressing NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages.
J Ethnopharmacol. 2009 Apr 21; 122(3):473-7.JE

Abstract

AIMS OF STUDY

Although the flowers of Chrysanthemum indicum Linné (Asteraceae) have long been used in traditional Korean and Chinese medicine to treat inflammatory diseases, the underlying mechanism(s) by which these effects are induced remains to be defined. We investigated the effects of a 70% ethanolic extract of C. indicum (CIE) on the activities of cellular signaling molecules that mediate inflammatory responses.

MATERIALS AND METHODS

Production of NO, PGE(2), TNF-alpha, and IL-1beta by ELISA, mRNA and protein expression of iNOS and COX-2, phosphorylation of MAPKs, and activation of NF-kappaB by RT-PCR and Western blotting were examined in LPS-induced RAW 264.7 macrophages.

RESULTS

The CIE strongly inhibited NO, PGE(2), TNF-alpha, and IL-1beta production, and also significantly inhibited mRNA and protein expression of iNOS and COX-2 in LPS-induced RAW 264.7 macrophages, in a dose-dependent manner. Furthermore, the CIE clearly suppressed nuclear translocation of NF-kappaB p65 subunits, which correlated with an inhibitory effect on IkappaBalpha phosphorylation. The CIE also attenuated the activation of ERK1/2 and JNK in a dose-dependent manner.

CONCLUSION

Our results suggest that the anti-inflammatory properties of CIE might result from the inhibition of inflammatory mediators, such as NO, PGE(2), TNF-alpha, and IL-1beta, via suppression of MAPKs and NF-kappaB-dependent pathways.

Authors+Show Affiliations

Department of Herbal Resources Research, Korea Institute of Oriental Medicine, Yuseong-gu, Daejeon, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19429315

Citation

Cheon, Myeong Sook, et al. "Chrysanthemum Indicum Linné Extract Inhibits the Inflammatory Response By Suppressing NF-kappaB and MAPKs Activation in Lipopolysaccharide-induced RAW 264.7 Macrophages." Journal of Ethnopharmacology, vol. 122, no. 3, 2009, pp. 473-7.
Cheon MS, Yoon T, Lee DY, et al. Chrysanthemum indicum Linné extract inhibits the inflammatory response by suppressing NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages. J Ethnopharmacol. 2009;122(3):473-7.
Cheon, M. S., Yoon, T., Lee, D. Y., Choi, G., Moon, B. C., Lee, A. Y., Choo, B. K., & Kim, H. K. (2009). Chrysanthemum indicum Linné extract inhibits the inflammatory response by suppressing NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages. Journal of Ethnopharmacology, 122(3), 473-7. https://doi.org/10.1016/j.jep.2009.01.034
Cheon MS, et al. Chrysanthemum Indicum Linné Extract Inhibits the Inflammatory Response By Suppressing NF-kappaB and MAPKs Activation in Lipopolysaccharide-induced RAW 264.7 Macrophages. J Ethnopharmacol. 2009 Apr 21;122(3):473-7. PubMed PMID: 19429315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chrysanthemum indicum Linné extract inhibits the inflammatory response by suppressing NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages. AU - Cheon,Myeong Sook, AU - Yoon,Taesook, AU - Lee,Do Yeon, AU - Choi,Goya, AU - Moon,Byeong Cheol, AU - Lee,A-Yeong, AU - Choo,Byung Kil, AU - Kim,Ho Kyoung, Y1 - 2009/02/03/ PY - 2008/07/03/received PY - 2008/12/23/revised PY - 2009/01/23/accepted PY - 2009/5/12/entrez PY - 2009/5/12/pubmed PY - 2009/8/6/medline SP - 473 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 122 IS - 3 N2 - AIMS OF STUDY: Although the flowers of Chrysanthemum indicum Linné (Asteraceae) have long been used in traditional Korean and Chinese medicine to treat inflammatory diseases, the underlying mechanism(s) by which these effects are induced remains to be defined. We investigated the effects of a 70% ethanolic extract of C. indicum (CIE) on the activities of cellular signaling molecules that mediate inflammatory responses. MATERIALS AND METHODS: Production of NO, PGE(2), TNF-alpha, and IL-1beta by ELISA, mRNA and protein expression of iNOS and COX-2, phosphorylation of MAPKs, and activation of NF-kappaB by RT-PCR and Western blotting were examined in LPS-induced RAW 264.7 macrophages. RESULTS: The CIE strongly inhibited NO, PGE(2), TNF-alpha, and IL-1beta production, and also significantly inhibited mRNA and protein expression of iNOS and COX-2 in LPS-induced RAW 264.7 macrophages, in a dose-dependent manner. Furthermore, the CIE clearly suppressed nuclear translocation of NF-kappaB p65 subunits, which correlated with an inhibitory effect on IkappaBalpha phosphorylation. The CIE also attenuated the activation of ERK1/2 and JNK in a dose-dependent manner. CONCLUSION: Our results suggest that the anti-inflammatory properties of CIE might result from the inhibition of inflammatory mediators, such as NO, PGE(2), TNF-alpha, and IL-1beta, via suppression of MAPKs and NF-kappaB-dependent pathways. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19429315/Chrysanthemum_indicum_Linné_extract_inhibits_the_inflammatory_response_by_suppressing_NF_kappaB_and_MAPKs_activation_in_lipopolysaccharide_induced_RAW_264_7_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00063-4 DB - PRIME DP - Unbound Medicine ER -