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Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells.
J Lipid Res. 2009 Sep; 50(9):1789-99.JL

Abstract

Carnitine palmitoyltransferase 1 (CPT1) catalyzes the first step in long-chain fatty acid import into mitochondria, and it is believed to be rate limiting for beta-oxidation of fatty acids. However, in muscle, other proteins may collaborate with CPT1. Fatty acid translocase/CD36 (FAT/CD36) may interact with CPT1 and contribute to fatty acid import into mitochondria in muscle. Here, we demonstrate that another membrane-bound fatty acid binding protein, fatty acid transport protein 1 (FATP1), collaborates with CPT1 for fatty acid import into mitochondria. Overexpression of FATP1 using adenovirus in L6E9 myotubes increased both fatty acid oxidation and palmitate esterification into triacylglycerides. Moreover, immunocytochemistry assays in transfected L6E9 myotubes showed that FATP1 was present in mitochondria and coimmunoprecipitated with CPT1 in L6E9 myotubes and rat skeletal muscle in vivo. The cooverexpression of FATP1 and CPT1 also enhanced mitochondrial fatty acid oxidation, similar to the cooverexpression of FAT/CD36 and CPT1. However, etomoxir, an irreversible inhibitor of CPT1, blocked all these effects. These data reveal that FATP1, like FAT/CD36, is associated with mitochondria and has a role in mitochondrial oxidation of fatty acids.

Authors+Show Affiliations

Departments of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19429947

Citation

Sebastián, David, et al. "Novel Role of FATP1 in Mitochondrial Fatty Acid Oxidation in Skeletal Muscle Cells." Journal of Lipid Research, vol. 50, no. 9, 2009, pp. 1789-99.
Sebastián D, Guitart M, García-Martínez C, et al. Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells. J Lipid Res. 2009;50(9):1789-99.
Sebastián, D., Guitart, M., García-Martínez, C., Mauvezin, C., Orellana-Gavaldà, J. M., Serra, D., Gómez-Foix, A. M., Hegardt, F. G., & Asins, G. (2009). Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells. Journal of Lipid Research, 50(9), 1789-99. https://doi.org/10.1194/jlr.M800535-JLR200
Sebastián D, et al. Novel Role of FATP1 in Mitochondrial Fatty Acid Oxidation in Skeletal Muscle Cells. J Lipid Res. 2009;50(9):1789-99. PubMed PMID: 19429947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells. AU - Sebastián,David, AU - Guitart,Maria, AU - García-Martínez,Celia, AU - Mauvezin,Caroline, AU - Orellana-Gavaldà,Josep M, AU - Serra,Dolors, AU - Gómez-Foix,Anna M, AU - Hegardt,Fausto G, AU - Asins,Guillermina, Y1 - 2009/05/09/ PY - 2009/5/12/entrez PY - 2009/5/12/pubmed PY - 2010/2/9/medline SP - 1789 EP - 99 JF - Journal of lipid research JO - J. Lipid Res. VL - 50 IS - 9 N2 - Carnitine palmitoyltransferase 1 (CPT1) catalyzes the first step in long-chain fatty acid import into mitochondria, and it is believed to be rate limiting for beta-oxidation of fatty acids. However, in muscle, other proteins may collaborate with CPT1. Fatty acid translocase/CD36 (FAT/CD36) may interact with CPT1 and contribute to fatty acid import into mitochondria in muscle. Here, we demonstrate that another membrane-bound fatty acid binding protein, fatty acid transport protein 1 (FATP1), collaborates with CPT1 for fatty acid import into mitochondria. Overexpression of FATP1 using adenovirus in L6E9 myotubes increased both fatty acid oxidation and palmitate esterification into triacylglycerides. Moreover, immunocytochemistry assays in transfected L6E9 myotubes showed that FATP1 was present in mitochondria and coimmunoprecipitated with CPT1 in L6E9 myotubes and rat skeletal muscle in vivo. The cooverexpression of FATP1 and CPT1 also enhanced mitochondrial fatty acid oxidation, similar to the cooverexpression of FAT/CD36 and CPT1. However, etomoxir, an irreversible inhibitor of CPT1, blocked all these effects. These data reveal that FATP1, like FAT/CD36, is associated with mitochondria and has a role in mitochondrial oxidation of fatty acids. SN - 1539-7262 UR - https://www.unboundmedicine.com/medline/citation/19429947/Novel_role_of_FATP1_in_mitochondrial_fatty_acid_oxidation_in_skeletal_muscle_cells_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=19429947 DB - PRIME DP - Unbound Medicine ER -