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Activation and stabilization of the hydroperoxide lyase enzymatic extract from mint leaves (Mentha spicata) using selected chemical additives.
Appl Biochem Biotechnol. 2010 Mar; 160(3):901-11.AB

Abstract

The effects of selected lyoprotecting excipients and chemical additives on the specific activity and the thermal stability of the hydroperoxide lyase (HPL) enzymatic extract from mint leaves were investigated. The addition of KCl (5%, w/w) and dextran (2.5%, w/w) to the enzymatic extract, prior to lyophilization, increased the HPL specific activity by 2.0- and 1.2-fold, respectively, compared to the control lyophilized extract. From half-life time (t (1/2)), it can be seen that KCl has enhanced the HPL stability by 1.3- to 2.3-fold, during long-period storage at -20 degrees Celsius and 4 degrees Celsius. Among the selected additives used throughout this study, glycine appeared to be the most effective one. In addition to the activation effect conferred by glycine, it also enhanced the HPL thermal stability. In contrast, polyhydroxyl-containing additives were not effective for stabilizing the HPL enzymatic extract. On the other hand, there was no signification increase in HPL activity and its thermal stability with the presence of Triton X-100. The results also showed that in the presence of glycine (10%), the catalytic efficiency of HPL was increased by 2.45-fold than that without additive.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Akacha NB
Biocatalysis and Bioprocess Unit, National Institute of Applied Science and Technology, BP 676, 1080 Tunis Cedex, Tunisia.
Karboune S
No affiliation info available
Gargouri M
No affiliation info available
Kermasha S
No affiliation info available

MeSH

Aldehyde-LyasesAnimalsBiocatalysisCytochrome P-450 Enzyme SystemEnzyme ActivationEnzyme StabilityFreeze DryingGreen Chemistry TechnologyInorganic ChemicalsKineticsMenthaOrganic ChemicalsPlant LeavesTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19430937

Citation

Akacha, Najla B., et al. "Activation and Stabilization of the Hydroperoxide Lyase Enzymatic Extract From Mint Leaves (Mentha Spicata) Using Selected Chemical Additives." Applied Biochemistry and Biotechnology, vol. 160, no. 3, 2010, pp. 901-11.
Akacha NB, Karboune S, Gargouri M, et al. Activation and stabilization of the hydroperoxide lyase enzymatic extract from mint leaves (Mentha spicata) using selected chemical additives. Appl Biochem Biotechnol. 2010;160(3):901-11.
Akacha, N. B., Karboune, S., Gargouri, M., & Kermasha, S. (2010). Activation and stabilization of the hydroperoxide lyase enzymatic extract from mint leaves (Mentha spicata) using selected chemical additives. Applied Biochemistry and Biotechnology, 160(3), 901-11. https://doi.org/10.1007/s12010-009-8625-9
Akacha NB, et al. Activation and Stabilization of the Hydroperoxide Lyase Enzymatic Extract From Mint Leaves (Mentha Spicata) Using Selected Chemical Additives. Appl Biochem Biotechnol. 2010;160(3):901-11. PubMed PMID: 19430937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation and stabilization of the hydroperoxide lyase enzymatic extract from mint leaves (Mentha spicata) using selected chemical additives. AU - Akacha,Najla B, AU - Karboune,Salwa, AU - Gargouri,Mohamed, AU - Kermasha,Selim, Y1 - 2009/05/11/ PY - 2008/11/04/received PY - 2009/03/24/accepted PY - 2009/5/12/entrez PY - 2009/5/12/pubmed PY - 2010/3/24/medline SP - 901 EP - 11 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 160 IS - 3 N2 - The effects of selected lyoprotecting excipients and chemical additives on the specific activity and the thermal stability of the hydroperoxide lyase (HPL) enzymatic extract from mint leaves were investigated. The addition of KCl (5%, w/w) and dextran (2.5%, w/w) to the enzymatic extract, prior to lyophilization, increased the HPL specific activity by 2.0- and 1.2-fold, respectively, compared to the control lyophilized extract. From half-life time (t (1/2)), it can be seen that KCl has enhanced the HPL stability by 1.3- to 2.3-fold, during long-period storage at -20 degrees Celsius and 4 degrees Celsius. Among the selected additives used throughout this study, glycine appeared to be the most effective one. In addition to the activation effect conferred by glycine, it also enhanced the HPL thermal stability. In contrast, polyhydroxyl-containing additives were not effective for stabilizing the HPL enzymatic extract. On the other hand, there was no signification increase in HPL activity and its thermal stability with the presence of Triton X-100. The results also showed that in the presence of glycine (10%), the catalytic efficiency of HPL was increased by 2.45-fold than that without additive. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/19430937/Activation_and_stabilization_of_the_hydroperoxide_lyase_enzymatic_extract_from_mint_leaves__Mentha_spicata__using_selected_chemical_additives_ L2 - https://dx.doi.org/10.1007/s12010-009-8625-9 DB - PRIME DP - Unbound Medicine ER -