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Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy.
Anal Biochem. 2009 Aug 01; 391(1):24-30.AB

Abstract

This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8mum diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology.

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Authors+Show Affiliations

Jun BH
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
Noh MS
No affiliation info available
Kim G
No affiliation info available
Kang H
No affiliation info available
Kim JH
No affiliation info available
Chung WJ
No affiliation info available
Kim MS
No affiliation info available
Kim YK
No affiliation info available
Cho MH
No affiliation info available
Jeong DH
No affiliation info available
Lee YS
No affiliation info available

MeSH

Aniline CompoundsBiotinMagneticsMetal NanoparticlesNaphthalenesPhenolsPolystyrenesProteinsSilverSpectrum Analysis, RamanStreptavidinSulfhydryl CompoundsSurface Properties

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19433055

Citation

Jun, Bong-Hyun, et al. "Protein Separation and Identification Using Magnetic Beads Encoded With Surface-enhanced Raman Spectroscopy." Analytical Biochemistry, vol. 391, no. 1, 2009, pp. 24-30.
Jun BH, Noh MS, Kim G, et al. Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy. Anal Biochem. 2009;391(1):24-30.
Jun, B. H., Noh, M. S., Kim, G., Kang, H., Kim, J. H., Chung, W. J., Kim, M. S., Kim, Y. K., Cho, M. H., Jeong, D. H., & Lee, Y. S. (2009). Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy. Analytical Biochemistry, 391(1), 24-30. https://doi.org/10.1016/j.ab.2009.05.005
Jun BH, et al. Protein Separation and Identification Using Magnetic Beads Encoded With Surface-enhanced Raman Spectroscopy. Anal Biochem. 2009 Aug 1;391(1):24-30. PubMed PMID: 19433055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy. AU - Jun,Bong-Hyun, AU - Noh,Mi Suk, AU - Kim,Gunsung, AU - Kang,Homan, AU - Kim,Jong-Ho, AU - Chung,Woo-Jae, AU - Kim,Min-Soo, AU - Kim,Yong-Kweon, AU - Cho,Myung-Haing, AU - Jeong,Dae Hong, AU - Lee,Yoon-Sik, Y1 - 2009/05/09/ PY - 2009/02/11/received PY - 2009/05/01/revised PY - 2009/05/05/accepted PY - 2009/5/13/entrez PY - 2009/5/13/pubmed PY - 2009/8/8/medline SP - 24 EP - 30 JF - Analytical biochemistry JO - Anal Biochem VL - 391 IS - 1 N2 - This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8mum diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/19433055/Protein_separation_and_identification_using_magnetic_beads_encoded_with_surface_enhanced_Raman_spectroscopy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(09)00323-6 DB - PRIME DP - Unbound Medicine ER -