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Seizure susceptibility and the brain regional sensitivity to oxidative stress in male and female rats in the lithium-pilocarpine model of temporal lobe epilepsy.

Abstract

Several studies have shown the existence of sex differences in the sensitivity to various convulsants in animals and to the development of some epilepsy types in humans. The purpose of this study was to investigate whether there are sex differences in seizure susceptibility and sensitivity of different brain regions to oxidative stress in rats with status epilepticus (SE) induced by lithium-pilocarpine administration, that provides a common experimental model of temporal lobe epilepsy (TLE) in humans. Latencies to isolated full limbic seizures or SE onset as well as the number of the animals presenting full limbic seizures, SE or full limbic seizures that progressed to SE were recorded for 2 h after pilocarpine administration. Number of animals which survived 24 h after SE onset was also monitored. Levels of lipid peroxidation as well as the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the piriform and entorhinal cortices, temporal neocortex, thalamus, and hippocampus in rats of both sexes, at 24 h after SE onset were determined. Results of our study showed that males developed full limbic seizures and SE more rapidly and in greater number than females. Levels of lipid peroxidation in all brain regions examined, the SOD activities in the piriform and entorhinal cortices, and temporal neocortex as well as the GSH-Px activities in the piriform and entorhinal cortices, and thalamus were significantly higher in rats with SE in comparison to the values of mentioned biochemical parameters in rats of the control groups. Lipid peroxidation level in the temporal neocortex as well as the GSH-Px activity in the hippocampus in male rats were significantly higher in comparison to the values registered in females. With the exception of the thalamus, where SOD activity in male rats with SE was significantly higher in relation to the respective control group and also to females with SE, sex differences in the response of other brain regions investigated to oxidative stress were not obtained, at 24 h after SE.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19439251

Citation

Peternel, Sandra, et al. "Seizure Susceptibility and the Brain Regional Sensitivity to Oxidative Stress in Male and Female Rats in the Lithium-pilocarpine Model of Temporal Lobe Epilepsy." Progress in Neuro-psychopharmacology & Biological Psychiatry, vol. 33, no. 3, 2009, pp. 456-62.
Peternel S, Pilipović K, Zupan G. Seizure susceptibility and the brain regional sensitivity to oxidative stress in male and female rats in the lithium-pilocarpine model of temporal lobe epilepsy. Prog Neuropsychopharmacol Biol Psychiatry. 2009;33(3):456-62.
Peternel, S., Pilipović, K., & Zupan, G. (2009). Seizure susceptibility and the brain regional sensitivity to oxidative stress in male and female rats in the lithium-pilocarpine model of temporal lobe epilepsy. Progress in Neuro-psychopharmacology & Biological Psychiatry, 33(3), pp. 456-62. doi:10.1016/j.pnpbp.2009.01.005.
Peternel S, Pilipović K, Zupan G. Seizure Susceptibility and the Brain Regional Sensitivity to Oxidative Stress in Male and Female Rats in the Lithium-pilocarpine Model of Temporal Lobe Epilepsy. Prog Neuropsychopharmacol Biol Psychiatry. 2009 Apr 30;33(3):456-62. PubMed PMID: 19439251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seizure susceptibility and the brain regional sensitivity to oxidative stress in male and female rats in the lithium-pilocarpine model of temporal lobe epilepsy. AU - Peternel,Sandra, AU - Pilipović,Kristina, AU - Zupan,Gordana, Y1 - 2009/01/21/ PY - 2008/06/19/received PY - 2008/12/24/revised PY - 2009/01/13/accepted PY - 2009/5/15/entrez PY - 2009/5/15/pubmed PY - 2009/6/17/medline SP - 456 EP - 62 JF - Progress in neuro-psychopharmacology & biological psychiatry JO - Prog. Neuropsychopharmacol. Biol. Psychiatry VL - 33 IS - 3 N2 - Several studies have shown the existence of sex differences in the sensitivity to various convulsants in animals and to the development of some epilepsy types in humans. The purpose of this study was to investigate whether there are sex differences in seizure susceptibility and sensitivity of different brain regions to oxidative stress in rats with status epilepticus (SE) induced by lithium-pilocarpine administration, that provides a common experimental model of temporal lobe epilepsy (TLE) in humans. Latencies to isolated full limbic seizures or SE onset as well as the number of the animals presenting full limbic seizures, SE or full limbic seizures that progressed to SE were recorded for 2 h after pilocarpine administration. Number of animals which survived 24 h after SE onset was also monitored. Levels of lipid peroxidation as well as the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the piriform and entorhinal cortices, temporal neocortex, thalamus, and hippocampus in rats of both sexes, at 24 h after SE onset were determined. Results of our study showed that males developed full limbic seizures and SE more rapidly and in greater number than females. Levels of lipid peroxidation in all brain regions examined, the SOD activities in the piriform and entorhinal cortices, and temporal neocortex as well as the GSH-Px activities in the piriform and entorhinal cortices, and thalamus were significantly higher in rats with SE in comparison to the values of mentioned biochemical parameters in rats of the control groups. Lipid peroxidation level in the temporal neocortex as well as the GSH-Px activity in the hippocampus in male rats were significantly higher in comparison to the values registered in females. With the exception of the thalamus, where SOD activity in male rats with SE was significantly higher in relation to the respective control group and also to females with SE, sex differences in the response of other brain regions investigated to oxidative stress were not obtained, at 24 h after SE. SN - 0278-5846 UR - https://www.unboundmedicine.com/medline/citation/19439251/Seizure_susceptibility_and_the_brain_regional_sensitivity_to_oxidative_stress_in_male_and_female_rats_in_the_lithium_pilocarpine_model_of_temporal_lobe_epilepsy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-5846(09)00012-8 DB - PRIME DP - Unbound Medicine ER -