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TRPC1 and TRPC6 channels cooperate with TRPV4 to mediate mechanical hyperalgesia and nociceptor sensitization.
J Neurosci. 2009 May 13; 29(19):6217-28.JN

Abstract

The transient receptor potential vanilloid 4 (TRPV4) contributes to mechanical hyperalgesia of diverse etiologies, presumably as part of a mechanoreceptor signaling complex (Alessandri-Haber et al., 2008). To investigate the hypothesis that a functional interaction between TRPV4 and stretch-activated ion channels (SACs) is involved in this mechanical transduction mechanism, we used a selective SACs inhibitor, GsMTx-4. Intradermal injection of GsMTx-4 in the rat hindpaw reversed the mechanical hyperalgesia induced by intradermal injection of inflammatory mediators. In vivo single fiber recordings showed that GsMTx-4 reversed inflammatory mediator-induced decrease in mechanical threshold in half of sensitized C-fibers. Furthermore, GsMTx-4 reduced hyperalgesia to both mechanical and hypotonic stimuli in different models of inflammatory and neuropathic pain, although it had no effect on baseline mechanical nociceptive thresholds. TRPC1 and TRPC6, two GsMTx-4-sensitive SACs, are expressed in dorsal root ganglion (DRG) neurons. Single-cell reverse transcription-PCR showed that messenger RNAs for TRPV4, TRPC1, and TRPC6 are frequently coexpressed in DRG neurons. Spinal intrathecal administration of oligodeoxynucleotides antisense to TRPC1 and TRPC6, like that to TRPV4, reversed the hyperalgesia to mechanical and hypotonic stimuli induced by inflammatory mediators without affecting baseline mechanical nociceptive threshold. However, antisense to TRPC6, but not to TRPC1, reversed the mechanical hyperalgesia induced by a thermal injury or the TRPV4-selective agonist 4alpha-PDD (4 alpha-phorbol 12,13-didecanoate). We conclude that TRPC1 and TRPC6 channels cooperate with TRPV4 channels to mediate mechanical hyperalgesia and primary afferent nociceptor sensitization, although they may have distinctive roles.

Authors+Show Affiliations

Department of Oral and Maxillofacial Surgery, University of California, San Francisco, California 94143-0440, USA. nicole.haber@ucsf.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19439599

Citation

Alessandri-Haber, Nicole, et al. "TRPC1 and TRPC6 Channels Cooperate With TRPV4 to Mediate Mechanical Hyperalgesia and Nociceptor Sensitization." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 29, no. 19, 2009, pp. 6217-28.
Alessandri-Haber N, Dina OA, Chen X, et al. TRPC1 and TRPC6 channels cooperate with TRPV4 to mediate mechanical hyperalgesia and nociceptor sensitization. J Neurosci. 2009;29(19):6217-28.
Alessandri-Haber, N., Dina, O. A., Chen, X., & Levine, J. D. (2009). TRPC1 and TRPC6 channels cooperate with TRPV4 to mediate mechanical hyperalgesia and nociceptor sensitization. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 29(19), 6217-28. https://doi.org/10.1523/JNEUROSCI.0893-09.2009
Alessandri-Haber N, et al. TRPC1 and TRPC6 Channels Cooperate With TRPV4 to Mediate Mechanical Hyperalgesia and Nociceptor Sensitization. J Neurosci. 2009 May 13;29(19):6217-28. PubMed PMID: 19439599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPC1 and TRPC6 channels cooperate with TRPV4 to mediate mechanical hyperalgesia and nociceptor sensitization. AU - Alessandri-Haber,Nicole, AU - Dina,Olayinka A, AU - Chen,Xiaoje, AU - Levine,Jon D, PY - 2009/5/15/entrez PY - 2009/5/15/pubmed PY - 2009/5/29/medline SP - 6217 EP - 28 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 29 IS - 19 N2 - The transient receptor potential vanilloid 4 (TRPV4) contributes to mechanical hyperalgesia of diverse etiologies, presumably as part of a mechanoreceptor signaling complex (Alessandri-Haber et al., 2008). To investigate the hypothesis that a functional interaction between TRPV4 and stretch-activated ion channels (SACs) is involved in this mechanical transduction mechanism, we used a selective SACs inhibitor, GsMTx-4. Intradermal injection of GsMTx-4 in the rat hindpaw reversed the mechanical hyperalgesia induced by intradermal injection of inflammatory mediators. In vivo single fiber recordings showed that GsMTx-4 reversed inflammatory mediator-induced decrease in mechanical threshold in half of sensitized C-fibers. Furthermore, GsMTx-4 reduced hyperalgesia to both mechanical and hypotonic stimuli in different models of inflammatory and neuropathic pain, although it had no effect on baseline mechanical nociceptive thresholds. TRPC1 and TRPC6, two GsMTx-4-sensitive SACs, are expressed in dorsal root ganglion (DRG) neurons. Single-cell reverse transcription-PCR showed that messenger RNAs for TRPV4, TRPC1, and TRPC6 are frequently coexpressed in DRG neurons. Spinal intrathecal administration of oligodeoxynucleotides antisense to TRPC1 and TRPC6, like that to TRPV4, reversed the hyperalgesia to mechanical and hypotonic stimuli induced by inflammatory mediators without affecting baseline mechanical nociceptive threshold. However, antisense to TRPC6, but not to TRPC1, reversed the mechanical hyperalgesia induced by a thermal injury or the TRPV4-selective agonist 4alpha-PDD (4 alpha-phorbol 12,13-didecanoate). We conclude that TRPC1 and TRPC6 channels cooperate with TRPV4 channels to mediate mechanical hyperalgesia and primary afferent nociceptor sensitization, although they may have distinctive roles. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/19439599/TRPC1_and_TRPC6_channels_cooperate_with_TRPV4_to_mediate_mechanical_hyperalgesia_and_nociceptor_sensitization_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=19439599 DB - PRIME DP - Unbound Medicine ER -