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Whole body hyperthermia reduces oxidative stress in the striatum of rats in an animal model of mitochondrial toxicity with 3-nitropropionic acid.
Int J Hyperthermia. 2009 Jun; 25(4):280-8.IJ

Abstract

PURPOSE

The goal of this study was to determine whether whole body hyperthermia (WBH) could reduce oxidative stress in the striatum produced by 3-nitropropionic acid (3-NP), a mitochondrial toxin that irreversibly inhibits succinate dehydrogenase (SDH), causing impairment of energy metabolism, oxidative stress and a selective degeneration of striatal cells.

METHODS

Rats were subjected to WBH (42 degrees C) or normothermia control conditions for 30 min and then treated with 3-NP. Striatum samples were processed and the levels of protein carbonyl groups, biogenic amines, Hsp72 and salicylate hydroxylation (to probe the hydroxyl radical (OH(*)) intervention) were determined.

RESULTS

WBH significantly reduced oxidative stress in the striatum of animals treated with 3-NP, as judged by reductions in protein carbonyl and salicylate hydroxylation derivative levels, whereas striatal Hsp72 expression was significantly increased. The groups treated with 3-NP presented an increased in the dopamine (DA) derivatives 2,3-dihydroxyphenylacetic acid (DOPAC) and norepinephrine (NE) concentration, whereas the striatal relation DOPAC/DA concentration indicate a reduced dopamine turnover.

CONCLUSIONS

These studies show, for the first time, that a heat shock pretreatment can ameliorate the oxidative stress produced by a metabolic toxin (3-NP) capable of impairing energy supply and produce selective striatal degeneration. These data contribute to a better understanding of the potential for thermal stress to modulate the type of oxidative stress usually present in neurodegenerative disorders associated with metabolic defects.

Authors+Show Affiliations

Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), C.P. 58261, Morelia, Michoacán, México. rafael.medina@imss.gob.mxNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19440936

Citation

Medina-Navarro, Rafael, and Israel Guerrero-Linares. "Whole Body Hyperthermia Reduces Oxidative Stress in the Striatum of Rats in an Animal Model of Mitochondrial Toxicity With 3-nitropropionic Acid." International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, vol. 25, no. 4, 2009, pp. 280-8.
Medina-Navarro R, Guerrero-Linares I. Whole body hyperthermia reduces oxidative stress in the striatum of rats in an animal model of mitochondrial toxicity with 3-nitropropionic acid. Int J Hyperthermia. 2009;25(4):280-8.
Medina-Navarro, R., & Guerrero-Linares, I. (2009). Whole body hyperthermia reduces oxidative stress in the striatum of rats in an animal model of mitochondrial toxicity with 3-nitropropionic acid. International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 25(4), 280-8. https://doi.org/10.1080/02656730902744387
Medina-Navarro R, Guerrero-Linares I. Whole Body Hyperthermia Reduces Oxidative Stress in the Striatum of Rats in an Animal Model of Mitochondrial Toxicity With 3-nitropropionic Acid. Int J Hyperthermia. 2009;25(4):280-8. PubMed PMID: 19440936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole body hyperthermia reduces oxidative stress in the striatum of rats in an animal model of mitochondrial toxicity with 3-nitropropionic acid. AU - Medina-Navarro,Rafael, AU - Guerrero-Linares,Israel, PY - 2009/5/15/entrez PY - 2009/5/15/pubmed PY - 2009/10/17/medline SP - 280 EP - 8 JF - International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group JO - Int J Hyperthermia VL - 25 IS - 4 N2 - PURPOSE: The goal of this study was to determine whether whole body hyperthermia (WBH) could reduce oxidative stress in the striatum produced by 3-nitropropionic acid (3-NP), a mitochondrial toxin that irreversibly inhibits succinate dehydrogenase (SDH), causing impairment of energy metabolism, oxidative stress and a selective degeneration of striatal cells. METHODS: Rats were subjected to WBH (42 degrees C) or normothermia control conditions for 30 min and then treated with 3-NP. Striatum samples were processed and the levels of protein carbonyl groups, biogenic amines, Hsp72 and salicylate hydroxylation (to probe the hydroxyl radical (OH(*)) intervention) were determined. RESULTS: WBH significantly reduced oxidative stress in the striatum of animals treated with 3-NP, as judged by reductions in protein carbonyl and salicylate hydroxylation derivative levels, whereas striatal Hsp72 expression was significantly increased. The groups treated with 3-NP presented an increased in the dopamine (DA) derivatives 2,3-dihydroxyphenylacetic acid (DOPAC) and norepinephrine (NE) concentration, whereas the striatal relation DOPAC/DA concentration indicate a reduced dopamine turnover. CONCLUSIONS: These studies show, for the first time, that a heat shock pretreatment can ameliorate the oxidative stress produced by a metabolic toxin (3-NP) capable of impairing energy supply and produce selective striatal degeneration. These data contribute to a better understanding of the potential for thermal stress to modulate the type of oxidative stress usually present in neurodegenerative disorders associated with metabolic defects. SN - 1464-5157 UR - https://www.unboundmedicine.com/medline/citation/19440936/Whole_body_hyperthermia_reduces_oxidative_stress_in_the_striatum_of_rats_in_an_animal_model_of_mitochondrial_toxicity_with_3_nitropropionic_acid_ L2 - https://www.tandfonline.com/doi/full/10.1080/02656730902744387 DB - PRIME DP - Unbound Medicine ER -