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Enantioselective determination of thyroxine enantiomers by ligand-exchange CE with UV absorbance and ICP-MS detection.
Electrophoresis. 2009 May; 30(10):1774-82.E

Abstract

A simple CE method has been developed for the separation and determination of thyroxine (T4) enantiomers in pharmaceutical formulations. The method was based on ligand-exchange mechanism using a Cu(II)/L-proline complex as chiral selector. The effects of different parameters affecting separation such as chiral selector concentration, organic additive, buffer pH and temperature were investigated. A baseline separation of the two enantiomers was obtained at a Cu(II)/L-proline ratio of 1:8 in a borate buffer (15 mmol/L, pH 9.6) containing 10% v/v acetonitrile. Under the optimized conditions, precision linearity range and detection limits of the developed enantioselective CE method were evaluated and compared using two different detection systems: conventional UV detection at 226 nm and iodine (127I)specific detection ("chiral speciation") with ICP-MS. Both methodologies show adequate analytical performance characteristics with detection limits around 0.30 microg/mL for each enantiomer of T4. Finally, a levothroid pharmaceutical formulation sample was successfully analyzed using both developed methods CE-UV and CE-ICP-MS.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kang J
Department of Physical and Analytical Chemistry, University of Oviedo, Oviedo, Spain.
Kutscher D
No affiliation info available
Montes-Bayón M
No affiliation info available
Blanco-González E
No affiliation info available
Sanz-Medel A
No affiliation info available

MeSH

Electrophoresis, CapillaryHydrogen-Ion ConcentrationLigandsMass SpectrometrySpectrophotometry, UltravioletStereoisomerismTemperatureThyroxine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19441034

Citation

Kang, Jianzhen, et al. "Enantioselective Determination of Thyroxine Enantiomers By Ligand-exchange CE With UV Absorbance and ICP-MS Detection." Electrophoresis, vol. 30, no. 10, 2009, pp. 1774-82.
Kang J, Kutscher D, Montes-Bayón M, et al. Enantioselective determination of thyroxine enantiomers by ligand-exchange CE with UV absorbance and ICP-MS detection. Electrophoresis. 2009;30(10):1774-82.
Kang, J., Kutscher, D., Montes-Bayón, M., Blanco-González, E., & Sanz-Medel, A. (2009). Enantioselective determination of thyroxine enantiomers by ligand-exchange CE with UV absorbance and ICP-MS detection. Electrophoresis, 30(10), 1774-82. https://doi.org/10.1002/elps.200800731
Kang J, et al. Enantioselective Determination of Thyroxine Enantiomers By Ligand-exchange CE With UV Absorbance and ICP-MS Detection. Electrophoresis. 2009;30(10):1774-82. PubMed PMID: 19441034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantioselective determination of thyroxine enantiomers by ligand-exchange CE with UV absorbance and ICP-MS detection. AU - Kang,Jianzhen, AU - Kutscher,Daniel, AU - Montes-Bayón,Maria, AU - Blanco-González,Elisa, AU - Sanz-Medel,Alfredo, PY - 2009/5/15/entrez PY - 2009/5/15/pubmed PY - 2009/10/23/medline SP - 1774 EP - 82 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 10 N2 - A simple CE method has been developed for the separation and determination of thyroxine (T4) enantiomers in pharmaceutical formulations. The method was based on ligand-exchange mechanism using a Cu(II)/L-proline complex as chiral selector. The effects of different parameters affecting separation such as chiral selector concentration, organic additive, buffer pH and temperature were investigated. A baseline separation of the two enantiomers was obtained at a Cu(II)/L-proline ratio of 1:8 in a borate buffer (15 mmol/L, pH 9.6) containing 10% v/v acetonitrile. Under the optimized conditions, precision linearity range and detection limits of the developed enantioselective CE method were evaluated and compared using two different detection systems: conventional UV detection at 226 nm and iodine (127I)specific detection ("chiral speciation") with ICP-MS. Both methodologies show adequate analytical performance characteristics with detection limits around 0.30 microg/mL for each enantiomer of T4. Finally, a levothroid pharmaceutical formulation sample was successfully analyzed using both developed methods CE-UV and CE-ICP-MS. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19441034/Enantioselective_determination_of_thyroxine_enantiomers_by_ligand_exchange_CE_with_UV_absorbance_and_ICP_MS_detection_ L2 - https://doi.org/10.1002/elps.200800731 DB - PRIME DP - Unbound Medicine ER -