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Acyl-CoA-induced generation of reactive oxygen species in mitochondrial preparations is due to the presence of peroxisomes.
Free Radic Biol Med. 2009 Sep 01; 47(5):503-9.FR

Abstract

Preparations of rat liver mitochondria, but not of brain and heart mitochondria, produce large quantities of reactive oxygen species (ROS) in the presence of palmitoyl-CoA and other long-chain acyl-CoAs. Palmitoyl-CoA inhibited respiration of rat liver mitochondria with glutamate plus malate or with succinate as substrate. However, ROS production induced by acyl-CoA was independent of respiration inhibition, as it was also observed in antimycin A- and rotenone-inhibited mitochondria and in submitochondrial particles in the absence of respiratory substrates (other than acyl-CoA). Increased ROS production by acyl-CoA in rat liver mitochondrial preparations was observed when measured in the external medium using Amplex red as a probe, but not inside mitochondria using the internal fluorescent probe MitoSOX or aconitase activity as the "intrinsic" indicator of ROS generation in the matrix compartment. Stimulation by acyl-CoA of ROS generation was higher in "light" mitochondrial preparations that were enriched in peroxisomes, as assayed by urate oxidase. It is concluded that stimulation of ROS production in preparations of rat liver mitochondria could be ascribed to contaminating peroxisomes. Preparations of rat brain and heart mitochondria were not or were much less contaminated with peroxisomes, as indicated by low urate oxidase activity.

Authors+Show Affiliations

Institut für Biochemie und Zellbiologie, Medizinische Fakultät, Otto-von-Guericke-Universität Magdeburg, 39120 Magdeburg, Germany. peter.schoenfeld@med.ovgu.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19442717

Citation

Schönfeld, Peter, et al. "Acyl-CoA-induced Generation of Reactive Oxygen Species in Mitochondrial Preparations Is Due to the Presence of Peroxisomes." Free Radical Biology & Medicine, vol. 47, no. 5, 2009, pp. 503-9.
Schönfeld P, Dymkowska D, Wojtczak L. Acyl-CoA-induced generation of reactive oxygen species in mitochondrial preparations is due to the presence of peroxisomes. Free Radic Biol Med. 2009;47(5):503-9.
Schönfeld, P., Dymkowska, D., & Wojtczak, L. (2009). Acyl-CoA-induced generation of reactive oxygen species in mitochondrial preparations is due to the presence of peroxisomes. Free Radical Biology & Medicine, 47(5), 503-9. https://doi.org/10.1016/j.freeradbiomed.2009.05.006
Schönfeld P, Dymkowska D, Wojtczak L. Acyl-CoA-induced Generation of Reactive Oxygen Species in Mitochondrial Preparations Is Due to the Presence of Peroxisomes. Free Radic Biol Med. 2009 Sep 1;47(5):503-9. PubMed PMID: 19442717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acyl-CoA-induced generation of reactive oxygen species in mitochondrial preparations is due to the presence of peroxisomes. AU - Schönfeld,Peter, AU - Dymkowska,Dorota, AU - Wojtczak,Lech, Y1 - 2009/05/13/ PY - 2009/03/05/received PY - 2009/04/29/revised PY - 2009/05/07/accepted PY - 2009/5/16/entrez PY - 2009/5/16/pubmed PY - 2009/12/16/medline SP - 503 EP - 9 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 47 IS - 5 N2 - Preparations of rat liver mitochondria, but not of brain and heart mitochondria, produce large quantities of reactive oxygen species (ROS) in the presence of palmitoyl-CoA and other long-chain acyl-CoAs. Palmitoyl-CoA inhibited respiration of rat liver mitochondria with glutamate plus malate or with succinate as substrate. However, ROS production induced by acyl-CoA was independent of respiration inhibition, as it was also observed in antimycin A- and rotenone-inhibited mitochondria and in submitochondrial particles in the absence of respiratory substrates (other than acyl-CoA). Increased ROS production by acyl-CoA in rat liver mitochondrial preparations was observed when measured in the external medium using Amplex red as a probe, but not inside mitochondria using the internal fluorescent probe MitoSOX or aconitase activity as the "intrinsic" indicator of ROS generation in the matrix compartment. Stimulation by acyl-CoA of ROS generation was higher in "light" mitochondrial preparations that were enriched in peroxisomes, as assayed by urate oxidase. It is concluded that stimulation of ROS production in preparations of rat liver mitochondria could be ascribed to contaminating peroxisomes. Preparations of rat brain and heart mitochondria were not or were much less contaminated with peroxisomes, as indicated by low urate oxidase activity. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/19442717/Acyl_CoA_induced_generation_of_reactive_oxygen_species_in_mitochondrial_preparations_is_due_to_the_presence_of_peroxisomes_ DB - PRIME DP - Unbound Medicine ER -