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An herbal decoction of Radix astragali and Radix angelicae sinensis promotes hematopoiesis and thrombopoiesis.
J Ethnopharmacol. 2009 Jul 06; 124(1):87-97.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

A decoction containing Radix angelicae sinensis and Radix astragali (Danggui Buxue Tang, DBT) has been used to raise the "Qi" and nourish the "Blood". However, its effects on haematopoiesis and particularly thrombopoiesis have not been studied.

AIMS

This study aims to examine the effects of DBT on hematopoiesis and thrombopoiesis.

MATERIALS AND METHODS

A myelosuppression mouse model was treated with DBT (10mg/kg/day). Peripheral blood cells from DBT and thrombopoietin-treated samples were counted on days 0, 7, 14 and 21. Then CFU assays were used to determine the effects of DBT on the megakaryocytic progenitor cells and other lineages. Last, analyses of annexin V, caspase-3, and mitochondrial membrane potential were conducted in megakaryocytic cell line M-07e.

RESULTS

Morphological examination showed that DBT treatment significantly increased the recovery of the megakaryocytic series. DBT significantly enhanced the platelet recovery and CFU-MK formation in vivo. DBT significantly promoted CFU-MK and CFU-F formation. Last, we observed the antiapoptotic effects of DBT on M-07e cells.

CONCLUSION

DBT might promote haematopoiesis and thrombopoiesis in the mouse model through (i) directly promoting the growth of megakaryocytes; (ii) indirectly promoting the growth of bone marrow stromal cells; (iii) inhibiting apoptosis of megakaryocytes.

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Authors+Show Affiliations

Yang M
Department of Paediatrics and Adolescent Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China. yangmo@hkucc.hku.hk
Chan GC
No affiliation info available
Deng R
No affiliation info available
Ng MH
No affiliation info available
Cheng SW
No affiliation info available
Lau CP
No affiliation info available
Ye JY
No affiliation info available
Wang L
No affiliation info available
Liu C
No affiliation info available

MeSH

Angelica sinensisAnimalsAnnexin A5ApoptosisAstragalus PlantBody WeightBone MarrowCaspase 3Cell LineDisease Models, AnimalDrugs, Chinese HerbalHematopoiesisIntracellular MembranesMaleMegakaryocytesMiceMice, Inbred BALB CMitochondriaOrgan SizePhytotherapyPlant ExtractsPlant RootsPlatelet CountRadiation Injuries, ExperimentalStem CellsThrombopoiesisThrombopoietin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19443149

Citation

Yang, Mo, et al. "An Herbal Decoction of Radix Astragali and Radix Angelicae Sinensis Promotes Hematopoiesis and Thrombopoiesis." Journal of Ethnopharmacology, vol. 124, no. 1, 2009, pp. 87-97.
Yang M, Chan GC, Deng R, et al. An herbal decoction of Radix astragali and Radix angelicae sinensis promotes hematopoiesis and thrombopoiesis. J Ethnopharmacol. 2009;124(1):87-97.
Yang, M., Chan, G. C., Deng, R., Ng, M. H., Cheng, S. W., Lau, C. P., Ye, J. Y., Wang, L., & Liu, C. (2009). An herbal decoction of Radix astragali and Radix angelicae sinensis promotes hematopoiesis and thrombopoiesis. Journal of Ethnopharmacology, 124(1), 87-97. https://doi.org/10.1016/j.jep.2009.04.007
Yang M, et al. An Herbal Decoction of Radix Astragali and Radix Angelicae Sinensis Promotes Hematopoiesis and Thrombopoiesis. J Ethnopharmacol. 2009 Jul 6;124(1):87-97. PubMed PMID: 19443149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An herbal decoction of Radix astragali and Radix angelicae sinensis promotes hematopoiesis and thrombopoiesis. AU - Yang,Mo, AU - Chan,Godfrey C F, AU - Deng,Ruixia, AU - Ng,Margaret H, AU - Cheng,Sau Wan, AU - Lau,Ching Po, AU - Ye,Jie Yu, AU - Wang,Liangjie, AU - Liu,Chang, Y1 - 2009/04/11/ PY - 2008/10/30/received PY - 2009/01/12/revised PY - 2009/04/03/accepted PY - 2009/5/16/entrez PY - 2009/5/16/pubmed PY - 2009/9/10/medline SP - 87 EP - 97 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 124 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: A decoction containing Radix angelicae sinensis and Radix astragali (Danggui Buxue Tang, DBT) has been used to raise the "Qi" and nourish the "Blood". However, its effects on haematopoiesis and particularly thrombopoiesis have not been studied. AIMS: This study aims to examine the effects of DBT on hematopoiesis and thrombopoiesis. MATERIALS AND METHODS: A myelosuppression mouse model was treated with DBT (10mg/kg/day). Peripheral blood cells from DBT and thrombopoietin-treated samples were counted on days 0, 7, 14 and 21. Then CFU assays were used to determine the effects of DBT on the megakaryocytic progenitor cells and other lineages. Last, analyses of annexin V, caspase-3, and mitochondrial membrane potential were conducted in megakaryocytic cell line M-07e. RESULTS: Morphological examination showed that DBT treatment significantly increased the recovery of the megakaryocytic series. DBT significantly enhanced the platelet recovery and CFU-MK formation in vivo. DBT significantly promoted CFU-MK and CFU-F formation. Last, we observed the antiapoptotic effects of DBT on M-07e cells. CONCLUSION: DBT might promote haematopoiesis and thrombopoiesis in the mouse model through (i) directly promoting the growth of megakaryocytes; (ii) indirectly promoting the growth of bone marrow stromal cells; (iii) inhibiting apoptosis of megakaryocytes. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19443149/An_herbal_decoction_of_Radix_astragali_and_Radix_angelicae_sinensis_promotes_hematopoiesis_and_thrombopoiesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00221-9 DB - PRIME DP - Unbound Medicine ER -
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