TY - JOUR T1 - [Study on the fingerprint of processed Rhizoma Atractylodis Macrocephalae by HPLC]. AU - Chen,Qin-hua, AU - Li,Peng, AU - He,Jing, AU - Zhu,Jun, AU - Chen,Shu-yan, PY - 2009/5/19/entrez PY - 2009/5/19/pubmed PY - 2009/9/5/medline SP - 35 EP - 8 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 32 IS - 1 N2 - OBJECTIVE: To establish HPLC fingerprint for the quality control of processed Rhizoma Atractylodis Macrocephalae (PRAM). METHODS: 14 batches of PRAM were collected from different places and were analyzed with the developed HPLC fingerprints method. The HPLC separation was performed on a Kromasil C18 analytical column (250 mm x 4.6 mm, 5 microm), and gradient elution was performed by mobile phase containing acetonitrile and water. The flow rate was 1.0 mL/min and the detection wavelength was at 242 nm. The temperature of column was 25 degrees C. RESULTS: Sixteen mutual peaks were selected in chromatography. Among the obtained fingerprints, the most of the detected peaks were separated effectively. The methodological evaluation showed that the method had a good repeatability. CONCLUSION: The RSD of relative retention time of mutual peaks which existed in all samples was less than 1.1%. The results of peak areas were in accordance with the request of fingerprint. The established fingerprint can be used for the quality control and species identifying of PRAM. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/19445118/[Study_on_the_fingerprint_of_processed_Rhizoma_Atractylodis_Macrocephalae_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -