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[Effects of Geranium sibirum extracts on liver metastases in nude mice with colonic cancer].
Zhong Yao Cai. 2009 Jan; 32(1):97-9.ZY

Abstract

OBJECTIVE

To establish nude mice models with the liver metastases of colonic adenocarcinoma and study the effects of Geranium sibirum extracts on them.

METHODS

Nude mice liver metastases model of colonic cancer was established with human colonic cancer cells line(Ls 174t) inoculated into mice spleen. 36 nude mice were randomly divided into 3 groups, containing control group, Geranium sibirum extracts group and hydroxycamptothecine group. The weight and size of the mice and growth of the carcinoma were recorded. All specimens were examined histologically. pS2 in blood in nude mice with liver metastasis of colonic carcinoma was detected with nested RT-PCR.

RESULTS

The incidence of liver metastasis was 100% in this intrasplenic injection model. The pathological results showed that tumour cells of liver metastases were poorly differentiated human colonic adenocarcinoma. In Geranium sibirum extracts group, the tumor number and the weight of liver metastases were significantly lower than those in hydroxycamptothecine group (P < 0.05). Using semiquantitative examination,in Geranium sibirum extracts group,the relative value of pS2 expression in blood was significantly higher than that in hydroxycamptothecine group (P < 0.05).

CONCLUSION

Geranium sibirum extracts can effectively inhibit the occurrence of liver metastases carcinoma and decrease the positive expression of pS2, it also has better effect than hydroxycamptothecine so that Geranium sibirum extracts may become the potential therapeutic strategy for liver metatstases of colonic cancer.

Authors+Show Affiliations

The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19445132

Citation

Huang, Guo-dong, et al. "[Effects of Geranium Sibirum Extracts On Liver Metastases in Nude Mice With Colonic Cancer]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 32, no. 1, 2009, pp. 97-9.
Huang GD, You Y, Huang YH, et al. [Effects of Geranium sibirum extracts on liver metastases in nude mice with colonic cancer]. Zhong Yao Cai. 2009;32(1):97-9.
Huang, G. D., You, Y., Huang, Y. H., Tang, L. J., Yang, Z. F., Xu, L., Huang, D. F., & Xiao, M. Z. (2009). [Effects of Geranium sibirum extracts on liver metastases in nude mice with colonic cancer]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 32(1), 97-9.
Huang GD, et al. [Effects of Geranium Sibirum Extracts On Liver Metastases in Nude Mice With Colonic Cancer]. Zhong Yao Cai. 2009;32(1):97-9. PubMed PMID: 19445132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Geranium sibirum extracts on liver metastases in nude mice with colonic cancer]. AU - Huang,Guo-dong, AU - You,Yu, AU - Huang,Yuan-hua, AU - Tang,Li-jun, AU - Yang,Zhi-fang, AU - Xu,Long, AU - Huang,Dao-fu, AU - Xiao,Mei-zhen, PY - 2009/5/19/entrez PY - 2009/5/19/pubmed PY - 2009/9/5/medline SP - 97 EP - 9 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 32 IS - 1 N2 - OBJECTIVE: To establish nude mice models with the liver metastases of colonic adenocarcinoma and study the effects of Geranium sibirum extracts on them. METHODS: Nude mice liver metastases model of colonic cancer was established with human colonic cancer cells line(Ls 174t) inoculated into mice spleen. 36 nude mice were randomly divided into 3 groups, containing control group, Geranium sibirum extracts group and hydroxycamptothecine group. The weight and size of the mice and growth of the carcinoma were recorded. All specimens were examined histologically. pS2 in blood in nude mice with liver metastasis of colonic carcinoma was detected with nested RT-PCR. RESULTS: The incidence of liver metastasis was 100% in this intrasplenic injection model. The pathological results showed that tumour cells of liver metastases were poorly differentiated human colonic adenocarcinoma. In Geranium sibirum extracts group, the tumor number and the weight of liver metastases were significantly lower than those in hydroxycamptothecine group (P < 0.05). Using semiquantitative examination,in Geranium sibirum extracts group,the relative value of pS2 expression in blood was significantly higher than that in hydroxycamptothecine group (P < 0.05). CONCLUSION: Geranium sibirum extracts can effectively inhibit the occurrence of liver metastases carcinoma and decrease the positive expression of pS2, it also has better effect than hydroxycamptothecine so that Geranium sibirum extracts may become the potential therapeutic strategy for liver metatstases of colonic cancer. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/19445132/[Effects_of_Geranium_sibirum_extracts_on_liver_metastases_in_nude_mice_with_colonic_cancer]_ L2 - http://www.diseaseinfosearch.org/result/4278 DB - PRIME DP - Unbound Medicine ER -