[Determination of 5-hydroxymethyl-furfural in root of Achyranthes bidentata expressing different degree of floating sugar].Zhongguo Zhong Yao Za Zhi. 2009 Feb; 34(3):298-300.ZZ
To study the relation between the content of 5-hydroxymethyl-furfural (5-HMF) and the degree of floating sugar in root of Achyranthes bidentata.
An HPLC method was applied with a Waters Symmetry C18 3.9 mm x 150 mm (5 microm) column by using methanol-water (12:88) as the mobile phase at a flow rate of 1.0 mL x min(-1) and a UV detection of 280 nm.
Along with the degree's deepening of floating sugar, the content of 5-HMF varied with the different shades of the sample. The content was 10 times higher in the black sample (highest degree of flowing suger) than that in the yellowish sample (normal). The concentrations of 5-HMT in five yellowish samples of roots of A. bidentata were 0.162 mg x g(-1) to 0.332 mg x g(-1).
The content increasing of 5-HMF in the root of A. bidentata was related to the degree of flowing sugar.