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Investigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets.
Vaccine. 2009 May 21; 27(24):3145-52.V

Abstract

To investigate the biological indicator for vaccine efficacy against HPAI H5N1 virus challenge of varying clades, two inactivated whole-virus H5N1 vaccines containing the hemagglutinin (HA) and neuraminidase (NA) genes of either clade 2.2 A/EM/Korea/W149/06 (RgKoreaW149/06 x PR8) or clade 2.5 A/Ck/Korea/ES/03 (RgKoreaES223N/03XPR8) virus in the background of A/PR/8/34 (H1N1) were generated by reverse genetics. Administration of the vaccines (2-dose 1.77, 3.5, 7.5 or 15microg of HA) elicited high HI titers in a dose-dependent manner. Mice immunized with RgKoreaW149/06 x PR8 were completely protected from challenge against wild-type A/EM/Korea/W149/06 without clinical signs of infection. RgKoreaES223N/03XPR8 could not protect mice at 1.77microg while all immunized ferrets were completely protected. Two-dose (7.5microg) vaccinated mice (HI titer > or =320) and triple dose (7.5 microg) vaccinated ferrets with RgKoreaES223N/03xPR8 (HI titer > or =640) protected vaccine recipients from mortality, inhibited nasal virus shedding and limited influenza virus tropism. Thus, these vaccines provided cross-protectivity in both models. More importantly, these results collectively suggested a positive correlation between vaccine-induced HI titers and inhibition of virus shedding including block of viral proliferation in major organs against a heterologous HPAI H5N1 virus. Although developing technologies or methods that will enable the reduction of administration dose/frequency remains to be resolved, our study demonstrated a considerable biological marker (> or =640 HI titer) for full protection of the vaccinated hosts that could provide a preliminary basis for the assessment of complete immunization.

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Authors+Show Affiliations

Song MS
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Oh TK
No affiliation info available
Pascua PN
No affiliation info available
Moon HJ
No affiliation info available
Lee JH
No affiliation info available
Baek YH
No affiliation info available
Woo KJ
No affiliation info available
Yoon Y
No affiliation info available
Sung MH
No affiliation info available
Poo H
No affiliation info available
Kim CJ
No affiliation info available
Choi YK
No affiliation info available

MeSH

AnimalsBiomarkersFerretsHemagglutination Inhibition TestsInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesMiceMice, Inbred BALB COrthomyxoviridae InfectionsVaccination

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19446184

Citation

Song, Min-Suk, et al. "Investigation of the Biological Indicator for Vaccine Efficacy Against Highly Pathogenic Avian Influenza (HPAI) H5N1 Virus Challenge in Mice and Ferrets." Vaccine, vol. 27, no. 24, 2009, pp. 3145-52.
Song MS, Oh TK, Pascua PN, et al. Investigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets. Vaccine. 2009;27(24):3145-52.
Song, M. S., Oh, T. K., Pascua, P. N., Moon, H. J., Lee, J. H., Baek, Y. H., Woo, K. J., Yoon, Y., Sung, M. H., Poo, H., Kim, C. J., & Choi, Y. K. (2009). Investigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets. Vaccine, 27(24), 3145-52. https://doi.org/10.1016/j.vaccine.2009.03.061
Song MS, et al. Investigation of the Biological Indicator for Vaccine Efficacy Against Highly Pathogenic Avian Influenza (HPAI) H5N1 Virus Challenge in Mice and Ferrets. Vaccine. 2009 May 21;27(24):3145-52. PubMed PMID: 19446184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of the biological indicator for vaccine efficacy against highly pathogenic avian influenza (HPAI) H5N1 virus challenge in mice and ferrets. AU - Song,Min-Suk, AU - Oh,Taek-Kyu, AU - Pascua,Philippe Noriel Q, AU - Moon,Ho-Jin, AU - Lee,Jun Han, AU - Baek,Yun Hee, AU - Woo,Kyu-Jin, AU - Yoon,Yeup, AU - Sung,Moon-Hee, AU - Poo,Haryoung, AU - Kim,Chul-Joong, AU - Choi,Young Ki, Y1 - 2009/04/09/ PY - 2008/12/26/received PY - 2009/03/13/revised PY - 2009/03/23/accepted PY - 2009/5/19/entrez PY - 2009/5/19/pubmed PY - 2009/7/8/medline SP - 3145 EP - 52 JF - Vaccine JO - Vaccine VL - 27 IS - 24 N2 - To investigate the biological indicator for vaccine efficacy against HPAI H5N1 virus challenge of varying clades, two inactivated whole-virus H5N1 vaccines containing the hemagglutinin (HA) and neuraminidase (NA) genes of either clade 2.2 A/EM/Korea/W149/06 (RgKoreaW149/06 x PR8) or clade 2.5 A/Ck/Korea/ES/03 (RgKoreaES223N/03XPR8) virus in the background of A/PR/8/34 (H1N1) were generated by reverse genetics. Administration of the vaccines (2-dose 1.77, 3.5, 7.5 or 15microg of HA) elicited high HI titers in a dose-dependent manner. Mice immunized with RgKoreaW149/06 x PR8 were completely protected from challenge against wild-type A/EM/Korea/W149/06 without clinical signs of infection. RgKoreaES223N/03XPR8 could not protect mice at 1.77microg while all immunized ferrets were completely protected. Two-dose (7.5microg) vaccinated mice (HI titer > or =320) and triple dose (7.5 microg) vaccinated ferrets with RgKoreaES223N/03xPR8 (HI titer > or =640) protected vaccine recipients from mortality, inhibited nasal virus shedding and limited influenza virus tropism. Thus, these vaccines provided cross-protectivity in both models. More importantly, these results collectively suggested a positive correlation between vaccine-induced HI titers and inhibition of virus shedding including block of viral proliferation in major organs against a heterologous HPAI H5N1 virus. Although developing technologies or methods that will enable the reduction of administration dose/frequency remains to be resolved, our study demonstrated a considerable biological marker (> or =640 HI titer) for full protection of the vaccinated hosts that could provide a preliminary basis for the assessment of complete immunization. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/19446184/Investigation_of_the_biological_indicator_for_vaccine_efficacy_against_highly_pathogenic_avian_influenza__HPAI__H5N1_virus_challenge_in_mice_and_ferrets_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(09)00486-1 DB - PRIME DP - Unbound Medicine ER -