TY - JOUR T1 - A pseudotype baculovirus-mediated vaccine confers protective immunity against lethal challenge with H5N1 avian influenza virus in mice and chickens. AU - Wu,Qunfeng, AU - Fang,Liurong, AU - Wu,Xuebao, AU - Li,Bin, AU - Luo,Rui, AU - Yu,Zhengjun, AU - Jin,Meilin, AU - Chen,Huanchun, AU - Xiao,Shaobo, Y1 - 2009/05/14/ PY - 2009/02/24/received PY - 2009/04/12/revised PY - 2009/04/16/accepted PY - 2009/5/19/entrez PY - 2009/5/19/pubmed PY - 2009/8/6/medline SP - 2210 EP - 7 JF - Molecular immunology JO - Mol Immunol VL - 46 IS - 11-12 N2 - Baculovirus has emerged recently as a novel and attractive gene delivery vehicle for mammalian cells. In this study, baculovirus pseudotyped with vesicular stomatitis virus glycoprotein was used as a vector to express the hemagglutinin (HA) protein of highly pathogenic H5N1 avian influenza virus, A/Chicken/Hubei/327/2004 (HB/327). The resultant recombinant baculovirus (BV-G-HA) mediated gene delivery and HA expression efficiently in mammalian cells. Mice immunized with 1 x 10(9)PFU of BV-G-HA developed significantly higher levels of H5-specific antibodies and cellular immunity than those that received 100 microg of DNA vaccines expressing HA, and were completely protected from lethal challenge with HB/327. Different vaccination doses were further tested in chickens, and these experiments demonstrated that 1 x 10(8)PFU of BV-G-HA offered complete protection from challenge with 100 LD(50) of HB/327. These data indicate that the pseudotype baculovirus-mediated vaccine could be utilized as an alternative strategy against the pandemic spread of H5N1 influenza virus. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/19446339/A_pseudotype_baculovirus_mediated_vaccine_confers_protective_immunity_against_lethal_challenge_with_H5N1_avian_influenza_virus_in_mice_and_chickens_ DB - PRIME DP - Unbound Medicine ER -