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Genetic modification of carbon catabolite repression in Trichoderma reesei for improved protein production.
Appl Environ Microbiol. 2009 Jul; 75(14):4853-60.AE

Abstract

The cellulase and hemicellulase genes of the filamentous fungus Trichoderma reesei have been shown to be under carbon catabolite repression mediated by the regulatory gene cre1. In this study, strains were constructed in which the cre1 gene was either completely removed or replaced by a truncated mutant variant, cre1-1, found previously in the Rut-C30 mutant strain with enhanced enzyme production capability. The T. reesei transformants with either deletion or truncation of cre1 had clearly altered colony morphology compared with the parental strains, forming smaller colonies and fewer aerial hyphae and spores. Liquid cultures in a medium with glucose as a carbon source showed that the transformants were derepressed in cellulase and hemicellulase production. Interestingly, they also produced significantly elevated levels of these hydrolytic enzymes in fermentations carried out in a medium inducing the hydrolase genes. This suggests that cre1 acts as a modulator of cellulase and hemicellulase gene expression under both noninducing and inducing conditions. There was no phenotypic difference between the Deltacre1 and cre1-1 mutant strains in any of the experiments done, indicating that the cre1-1 gene is practically a null allele. The results of this work indicate that cre1 is a valid target gene in strain engineering for improved enzyme production in T. reesei.

Authors+Show Affiliations

VTT Technical Research Centre, P.O. Box 1000, FI-02044 VTT, Espoo, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19447952

Citation

Nakari-Setälä, Tiina, et al. "Genetic Modification of Carbon Catabolite Repression in Trichoderma Reesei for Improved Protein Production." Applied and Environmental Microbiology, vol. 75, no. 14, 2009, pp. 4853-60.
Nakari-Setälä T, Paloheimo M, Kallio J, et al. Genetic modification of carbon catabolite repression in Trichoderma reesei for improved protein production. Appl Environ Microbiol. 2009;75(14):4853-60.
Nakari-Setälä, T., Paloheimo, M., Kallio, J., Vehmaanperä, J., Penttilä, M., & Saloheimo, M. (2009). Genetic modification of carbon catabolite repression in Trichoderma reesei for improved protein production. Applied and Environmental Microbiology, 75(14), 4853-60. https://doi.org/10.1128/AEM.00282-09
Nakari-Setälä T, et al. Genetic Modification of Carbon Catabolite Repression in Trichoderma Reesei for Improved Protein Production. Appl Environ Microbiol. 2009;75(14):4853-60. PubMed PMID: 19447952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic modification of carbon catabolite repression in Trichoderma reesei for improved protein production. AU - Nakari-Setälä,Tiina, AU - Paloheimo,Marja, AU - Kallio,Jarno, AU - Vehmaanperä,Jari, AU - Penttilä,Merja, AU - Saloheimo,Markku, Y1 - 2009/05/15/ PY - 2009/5/19/entrez PY - 2009/5/19/pubmed PY - 2009/9/29/medline SP - 4853 EP - 60 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 75 IS - 14 N2 - The cellulase and hemicellulase genes of the filamentous fungus Trichoderma reesei have been shown to be under carbon catabolite repression mediated by the regulatory gene cre1. In this study, strains were constructed in which the cre1 gene was either completely removed or replaced by a truncated mutant variant, cre1-1, found previously in the Rut-C30 mutant strain with enhanced enzyme production capability. The T. reesei transformants with either deletion or truncation of cre1 had clearly altered colony morphology compared with the parental strains, forming smaller colonies and fewer aerial hyphae and spores. Liquid cultures in a medium with glucose as a carbon source showed that the transformants were derepressed in cellulase and hemicellulase production. Interestingly, they also produced significantly elevated levels of these hydrolytic enzymes in fermentations carried out in a medium inducing the hydrolase genes. This suggests that cre1 acts as a modulator of cellulase and hemicellulase gene expression under both noninducing and inducing conditions. There was no phenotypic difference between the Deltacre1 and cre1-1 mutant strains in any of the experiments done, indicating that the cre1-1 gene is practically a null allele. The results of this work indicate that cre1 is a valid target gene in strain engineering for improved enzyme production in T. reesei. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/19447952/Genetic_modification_of_carbon_catabolite_repression_in_Trichoderma_reesei_for_improved_protein_production_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=19447952 DB - PRIME DP - Unbound Medicine ER -