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Nociceptin modulates bronchoconstriction induced by sensory nerve activation in mouse lung.
Am J Respir Cell Mol Biol. 2010 Feb; 42(2):250-4.AJ

Abstract

Nociceptin/orphanin FQ (N/OFQ), the endogenous ligand for the N/OFQ peptide receptor (NOP), inhibits tachykinin release in the airway of several animal models. The aim of this study was to investigate the role of the N/OFQ-NOP receptor system in bronchoconstriction induced by sensory nerve activation in the isolated mouse lung. We used C57BL/6J NOP(+/+), NOP(-/-), and Balb/C mice sensitized (or not) to ovalbumin. Bronchopulmonary function coupled with measurements of endogenous N/OFQ levels before and after capsaicin-induced bronchoconstriction in the presence or absence of NOP-selective agonists/antagonists are presented. N/OFQ significantly inhibited capsaicin-induced bronchoconstriction in both naive and sensitized mice, these latter animals displaying airway hyperresponsiveness to capsaicin. The inhibitory effect of N/OFQ were not observed in NOP(-/-) mice, and were mimicked/abolished by the selective NOP agonist/antagonist University of Ferrara Peptide (UFP)-112/UFP-101 in NOP(+/+) mice. UFP-101 alone potentiated the effect of capsaicin in naive mice, but not in sensitized mice. Endogenous N/OFQ levels significantly decreased in sensitized mice relative to naive mice. We have demonstrated that a reduction in endogenous N/OFQ, or the lack of its receptor, causes an increase in capsaicin-induced bronchoconstriction, implying a role for the N/OFQ-NOP receptor system in the modulation of capsaicin effects. Moreover, for the first time, we document differential airway responsiveness to capsaicin between naive and sensitized mice due, at least in part, to decreased endogenous N/OFQ levels in sensitized mice.

Authors+Show Affiliations

Department of Experimental Medicine, sect. of Pharmacology "L.Donatelli", Faculty of Medicine and Surgery, Second University of Naples, via Costantinopoli 16, 80138 Naples, Italy. bruno.dagostino@unina2.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19448152

Citation

D'Agostino, Bruno, et al. "Nociceptin Modulates Bronchoconstriction Induced By Sensory Nerve Activation in Mouse Lung." American Journal of Respiratory Cell and Molecular Biology, vol. 42, no. 2, 2010, pp. 250-4.
D'Agostino B, Orlotti D, Calò G, et al. Nociceptin modulates bronchoconstriction induced by sensory nerve activation in mouse lung. Am J Respir Cell Mol Biol. 2010;42(2):250-4.
D'Agostino, B., Orlotti, D., Calò, G., Sullo, N., Russo, M., Guerrini, R., De Nardo, M., Mazzeo, F., Candeletti, S., & Rossi, F. (2010). Nociceptin modulates bronchoconstriction induced by sensory nerve activation in mouse lung. American Journal of Respiratory Cell and Molecular Biology, 42(2), 250-4. https://doi.org/10.1165/rcmb.2008-0488OC
D'Agostino B, et al. Nociceptin Modulates Bronchoconstriction Induced By Sensory Nerve Activation in Mouse Lung. Am J Respir Cell Mol Biol. 2010;42(2):250-4. PubMed PMID: 19448152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nociceptin modulates bronchoconstriction induced by sensory nerve activation in mouse lung. AU - D'Agostino,Bruno, AU - Orlotti,Donatella, AU - Calò,Girolamo, AU - Sullo,Nikol, AU - Russo,Mariangela, AU - Guerrini,Remo, AU - De Nardo,Marilisa, AU - Mazzeo,Filomena, AU - Candeletti,Sanzio, AU - Rossi,Francesco, Y1 - 2009/05/15/ PY - 2009/5/19/entrez PY - 2009/5/19/pubmed PY - 2010/2/2/medline SP - 250 EP - 4 JF - American journal of respiratory cell and molecular biology JO - Am. J. Respir. Cell Mol. Biol. VL - 42 IS - 2 N2 - Nociceptin/orphanin FQ (N/OFQ), the endogenous ligand for the N/OFQ peptide receptor (NOP), inhibits tachykinin release in the airway of several animal models. The aim of this study was to investigate the role of the N/OFQ-NOP receptor system in bronchoconstriction induced by sensory nerve activation in the isolated mouse lung. We used C57BL/6J NOP(+/+), NOP(-/-), and Balb/C mice sensitized (or not) to ovalbumin. Bronchopulmonary function coupled with measurements of endogenous N/OFQ levels before and after capsaicin-induced bronchoconstriction in the presence or absence of NOP-selective agonists/antagonists are presented. N/OFQ significantly inhibited capsaicin-induced bronchoconstriction in both naive and sensitized mice, these latter animals displaying airway hyperresponsiveness to capsaicin. The inhibitory effect of N/OFQ were not observed in NOP(-/-) mice, and were mimicked/abolished by the selective NOP agonist/antagonist University of Ferrara Peptide (UFP)-112/UFP-101 in NOP(+/+) mice. UFP-101 alone potentiated the effect of capsaicin in naive mice, but not in sensitized mice. Endogenous N/OFQ levels significantly decreased in sensitized mice relative to naive mice. We have demonstrated that a reduction in endogenous N/OFQ, or the lack of its receptor, causes an increase in capsaicin-induced bronchoconstriction, implying a role for the N/OFQ-NOP receptor system in the modulation of capsaicin effects. Moreover, for the first time, we document differential airway responsiveness to capsaicin between naive and sensitized mice due, at least in part, to decreased endogenous N/OFQ levels in sensitized mice. SN - 1535-4989 UR - https://www.unboundmedicine.com/medline/citation/19448152/Nociceptin_modulates_bronchoconstriction_induced_by_sensory_nerve_activation_in_mouse_lung_ L2 - http://www.atsjournals.org/doi/full/10.1165/rcmb.2008-0488OC?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -