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Role of LOX-1 in monocyte adhesion-triggered redox, Akt/eNOS and Ca2+ signaling pathways in endothelial cells.
J Cell Physiol. 2009 Sep; 220(3):706-15.JC

Abstract

This study was conducted to examine the role of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in monocyte adhesion-induced redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in endothelial cells (ECs). LOX-1 was blocked by an antibody-neutralizing LOX-1 TS92 or small interfering RNA. In cultured human aortic ECs, monocyte adhesion activated Rac1 and p47(phox), and increased NADPH oxidase activity and reactive oxygen species (ROS) generation within 30 min and NF-kappaB phosphorylation within 1 h, resulting in redox-sensitive gene expression. Akt and eNOS phosphorylation was induced 15 min after adding monocytes and returned to control level after 30 min, whereas NO production was not altered by monocyte adhesion. Blockade of LOX-1 blunted the monocyte adhesion-triggered redox-sensitive signaling pathway and Akt/eNOS phosphorylation in ECs. Both endothelial intracellular Ca2+ mobilization and Ca2+ influx caused by monocyte attachment were markedly attenuated by pretreatment of ECs with TS92. This suggests that LOX-1 is involved in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of oxidized low-density lipoprotein (ox-LDL). Furthermore, blockade of Ca2+ inhibited monocyte adhesion-triggered Rac1 and p47(phox) activation and ROS generation in ECs, whereas Ca2+ signaling was suppressed by blockade of NADPH oxidase and ROS generation. Finally, TS92 blocked the monocyte adhesion to ECs stimulated with or without tumor necrosis factor-alpha or ox-LDL. We provide evidence that LOX-1 plays a role in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of the ox-LDL-LOX-1 axis.

Authors+Show Affiliations

First Department of Internal Medicine, Fukushima Medical University, Fukushima 960-1295, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19452449

Citation

Sakamoto, Nobuo, et al. "Role of LOX-1 in Monocyte Adhesion-triggered Redox, Akt/eNOS and Ca2+ Signaling Pathways in Endothelial Cells." Journal of Cellular Physiology, vol. 220, no. 3, 2009, pp. 706-15.
Sakamoto N, Ishibashi T, Sugimoto K, et al. Role of LOX-1 in monocyte adhesion-triggered redox, Akt/eNOS and Ca2+ signaling pathways in endothelial cells. J Cell Physiol. 2009;220(3):706-15.
Sakamoto, N., Ishibashi, T., Sugimoto, K., Sawamura, T., Sakamoto, T., Inoue, N., Saitoh, S., Kamioka, M., Uekita, H., Ohkawara, H., Suzuki, K., Teramoto, T., Maruyama, Y., & Takeishi, Y. (2009). Role of LOX-1 in monocyte adhesion-triggered redox, Akt/eNOS and Ca2+ signaling pathways in endothelial cells. Journal of Cellular Physiology, 220(3), 706-15. https://doi.org/10.1002/jcp.21818
Sakamoto N, et al. Role of LOX-1 in Monocyte Adhesion-triggered Redox, Akt/eNOS and Ca2+ Signaling Pathways in Endothelial Cells. J Cell Physiol. 2009;220(3):706-15. PubMed PMID: 19452449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of LOX-1 in monocyte adhesion-triggered redox, Akt/eNOS and Ca2+ signaling pathways in endothelial cells. AU - Sakamoto,Nobuo, AU - Ishibashi,Toshiyuki, AU - Sugimoto,Koichi, AU - Sawamura,Tatsuya, AU - Sakamoto,Takayuki, AU - Inoue,Nobutaka, AU - Saitoh,Shu-Ichi, AU - Kamioka,Masashi, AU - Uekita,Hironori, AU - Ohkawara,Hiroshi, AU - Suzuki,Koji, AU - Teramoto,Tamio, AU - Maruyama,Yukio, AU - Takeishi,Yasuchika, PY - 2009/5/20/entrez PY - 2009/5/20/pubmed PY - 2009/7/17/medline SP - 706 EP - 15 JF - Journal of cellular physiology JO - J Cell Physiol VL - 220 IS - 3 N2 - This study was conducted to examine the role of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in monocyte adhesion-induced redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in endothelial cells (ECs). LOX-1 was blocked by an antibody-neutralizing LOX-1 TS92 or small interfering RNA. In cultured human aortic ECs, monocyte adhesion activated Rac1 and p47(phox), and increased NADPH oxidase activity and reactive oxygen species (ROS) generation within 30 min and NF-kappaB phosphorylation within 1 h, resulting in redox-sensitive gene expression. Akt and eNOS phosphorylation was induced 15 min after adding monocytes and returned to control level after 30 min, whereas NO production was not altered by monocyte adhesion. Blockade of LOX-1 blunted the monocyte adhesion-triggered redox-sensitive signaling pathway and Akt/eNOS phosphorylation in ECs. Both endothelial intracellular Ca2+ mobilization and Ca2+ influx caused by monocyte attachment were markedly attenuated by pretreatment of ECs with TS92. This suggests that LOX-1 is involved in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of oxidized low-density lipoprotein (ox-LDL). Furthermore, blockade of Ca2+ inhibited monocyte adhesion-triggered Rac1 and p47(phox) activation and ROS generation in ECs, whereas Ca2+ signaling was suppressed by blockade of NADPH oxidase and ROS generation. Finally, TS92 blocked the monocyte adhesion to ECs stimulated with or without tumor necrosis factor-alpha or ox-LDL. We provide evidence that LOX-1 plays a role in redox-sensitive, Akt/eNOS and Ca2+ signaling pathways in monocyte adhesion to ECs independent of the ox-LDL-LOX-1 axis. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/19452449/Role_of_LOX_1_in_monocyte_adhesion_triggered_redox_Akt/eNOS_and_Ca2+_signaling_pathways_in_endothelial_cells_ L2 - https://doi.org/10.1002/jcp.21818 DB - PRIME DP - Unbound Medicine ER -