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[Determination of degree of esterification in pectic polysaccharides from Angelica sinensis (Oliv.) diels by Fourier transform infrared spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Mar; 29(3):682-5.GP

Abstract

An infrared spectroscopy method for rapid quantitative determination of the degree of esterification (DE) of pectic polysaccharides was developed. Taking pectic polysaccharides from angelica sinensis as samples, the calibration curve was established between DE and the ratio of A1 730/(A1 730 + A1 630). The square of the linear correlation coefficient was 0.822, indicating a good linear relationship between the DE and the absorbent area at 1 730 and 1 630 cm(-1). Using this method, the DE of pectic polysaccharides from angelica sinensis was determined to be between 42.36% and 54.06% for crude and purified samples respectively. The relationship between infrared spectroscopy data and titrimetrically determined DE values was investigated, indicating an excellent reproducibility. This method is characterized by less sample, simple manipulation, higher sensibility, and speedy analysis compared to chemical method and other instrumentations. FTIR shows a good feasibility and can be a rapid, alternative method to titrimetric analysis for DE determination.

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Authors+Show Affiliations

Sun YL
Department of Life Science, Yuncheng University, Yuncheng 044000, China. sylwts@yahoo.com.cn
Cui WW
No affiliation info available
Gu XH
No affiliation info available
Tang J
No affiliation info available
Shen RL
No affiliation info available

MeSH

AbsorptionAngelica sinensisCalibrationEsterificationPectinsSpectroscopy, Fourier Transform Infrared

Pub Type(s)

Journal Article

Language

chi

PubMed ID

19455799

Citation

Sun, Yuan-Lin, et al. "[Determination of Degree of Esterification in Pectic Polysaccharides From Angelica Sinensis (Oliv.) Diels By Fourier Transform Infrared Spectroscopy]." Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu, vol. 29, no. 3, 2009, pp. 682-5.
Sun YL, Cui WW, Gu XH, et al. [Determination of degree of esterification in pectic polysaccharides from Angelica sinensis (Oliv.) diels by Fourier transform infrared spectroscopy]. Guang Pu Xue Yu Guang Pu Fen Xi. 2009;29(3):682-5.
Sun, Y. L., Cui, W. W., Gu, X. H., Tang, J., & Shen, R. L. (2009). [Determination of degree of esterification in pectic polysaccharides from Angelica sinensis (Oliv.) diels by Fourier transform infrared spectroscopy]. Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu, 29(3), 682-5.
Sun YL, et al. [Determination of Degree of Esterification in Pectic Polysaccharides From Angelica Sinensis (Oliv.) Diels By Fourier Transform Infrared Spectroscopy]. Guang Pu Xue Yu Guang Pu Fen Xi. 2009;29(3):682-5. PubMed PMID: 19455799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of degree of esterification in pectic polysaccharides from Angelica sinensis (Oliv.) diels by Fourier transform infrared spectroscopy]. AU - Sun,Yuan-Lin, AU - Cui,Wu-Wei, AU - Gu,Xiao-Hong, AU - Tang,Jian, AU - Shen,Rui-Ling, PY - 2009/5/22/entrez PY - 2009/5/22/pubmed PY - 2010/6/11/medline SP - 682 EP - 5 JF - Guang pu xue yu guang pu fen xi = Guang pu JO - Guang Pu Xue Yu Guang Pu Fen Xi VL - 29 IS - 3 N2 - An infrared spectroscopy method for rapid quantitative determination of the degree of esterification (DE) of pectic polysaccharides was developed. Taking pectic polysaccharides from angelica sinensis as samples, the calibration curve was established between DE and the ratio of A1 730/(A1 730 + A1 630). The square of the linear correlation coefficient was 0.822, indicating a good linear relationship between the DE and the absorbent area at 1 730 and 1 630 cm(-1). Using this method, the DE of pectic polysaccharides from angelica sinensis was determined to be between 42.36% and 54.06% for crude and purified samples respectively. The relationship between infrared spectroscopy data and titrimetrically determined DE values was investigated, indicating an excellent reproducibility. This method is characterized by less sample, simple manipulation, higher sensibility, and speedy analysis compared to chemical method and other instrumentations. FTIR shows a good feasibility and can be a rapid, alternative method to titrimetric analysis for DE determination. SN - 1000-0593 UR - https://www.unboundmedicine.com/medline/citation/19455799/[Determination_of_degree_of_esterification_in_pectic_polysaccharides_from_Angelica_sinensis__Oliv___diels_by_Fourier_transform_infrared_spectroscopy]_ L2 - https://www.ingentaconnect.com/openurl?genre=article&issn=1000-0593&volume=29&issue=3&spage=682&aulast=Sun DB - PRIME DP - Unbound Medicine ER -