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Detection of cocaine and its metabolites in urine using solid phase extraction-ion mobility spectrometry with alternating least squares.
Forensic Sci Int. 2009 Aug 10; 189(1-3):54-9.FS

Abstract

A reliable, alternative screening method for detection of cocaine and its metabolites, benzoylecgonine and cocaethylene in urine is demonstrated using solid phase extraction (SPE) coupled with ion mobility spectrometry (IMS). Data analysis with alternating least squares (ALS) is used to model IMS spectral datasets and separate the reactant ion peak from the product ion peaks. IMS has been used as a screening device for drug and explosive detection for many years. It has the advantages of atmospheric pressure operation, simple sample preparation, portability, fast analysis, and high sensitivity when compared to similar methods. Coupling SPE with IMS decreases the detection limits of drug metabolites in urine while removing salts and other polar compounds that suppress ionization during the measurement. The IMS analysis time in this experiment is 20s, much shorter than traditional chromatographic analysis. The application of ALS further increases the sensitivity and selectivity of this method. The detection limits of benzoylecgonine and cocaethylene are 10 ng/mL and 4 ng/mL, respectively. Commercial adulteration of urine specimens does not influence the ability to detect cocaine metabolites after sampling the urine with SPE. This method provides forensic chemists a viable approach for fast and simple drug screening.

Authors+Show Affiliations

Clippinger Laboratories, Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701-2979, United States.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19457629

Citation

Lu, Yao, et al. "Detection of Cocaine and Its Metabolites in Urine Using Solid Phase Extraction-ion Mobility Spectrometry With Alternating Least Squares." Forensic Science International, vol. 189, no. 1-3, 2009, pp. 54-9.
Lu Y, O'Donnell RM, Harrington PB. Detection of cocaine and its metabolites in urine using solid phase extraction-ion mobility spectrometry with alternating least squares. Forensic Sci Int. 2009;189(1-3):54-9.
Lu, Y., O'Donnell, R. M., & Harrington, P. B. (2009). Detection of cocaine and its metabolites in urine using solid phase extraction-ion mobility spectrometry with alternating least squares. Forensic Science International, 189(1-3), 54-9. https://doi.org/10.1016/j.forsciint.2009.04.007
Lu Y, O'Donnell RM, Harrington PB. Detection of Cocaine and Its Metabolites in Urine Using Solid Phase Extraction-ion Mobility Spectrometry With Alternating Least Squares. Forensic Sci Int. 2009 Aug 10;189(1-3):54-9. PubMed PMID: 19457629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of cocaine and its metabolites in urine using solid phase extraction-ion mobility spectrometry with alternating least squares. AU - Lu,Yao, AU - O'Donnell,Ryan M, AU - Harrington,Peter B, Y1 - 2009/05/19/ PY - 2008/09/08/received PY - 2009/04/02/revised PY - 2009/04/04/accepted PY - 2009/5/22/entrez PY - 2009/5/22/pubmed PY - 2009/11/11/medline SP - 54 EP - 9 JF - Forensic science international JO - Forensic Sci Int VL - 189 IS - 1-3 N2 - A reliable, alternative screening method for detection of cocaine and its metabolites, benzoylecgonine and cocaethylene in urine is demonstrated using solid phase extraction (SPE) coupled with ion mobility spectrometry (IMS). Data analysis with alternating least squares (ALS) is used to model IMS spectral datasets and separate the reactant ion peak from the product ion peaks. IMS has been used as a screening device for drug and explosive detection for many years. It has the advantages of atmospheric pressure operation, simple sample preparation, portability, fast analysis, and high sensitivity when compared to similar methods. Coupling SPE with IMS decreases the detection limits of drug metabolites in urine while removing salts and other polar compounds that suppress ionization during the measurement. The IMS analysis time in this experiment is 20s, much shorter than traditional chromatographic analysis. The application of ALS further increases the sensitivity and selectivity of this method. The detection limits of benzoylecgonine and cocaethylene are 10 ng/mL and 4 ng/mL, respectively. Commercial adulteration of urine specimens does not influence the ability to detect cocaine metabolites after sampling the urine with SPE. This method provides forensic chemists a viable approach for fast and simple drug screening. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/19457629/Detection_of_cocaine_and_its_metabolites_in_urine_using_solid_phase_extraction_ion_mobility_spectrometry_with_alternating_least_squares_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(09)00163-7 DB - PRIME DP - Unbound Medicine ER -