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Co-administration of nerve growth factor and butyrate regulates vanilloid receptor 1 and substance P levels in cultures of rat dorsal root ganglion neurons.
Acta Physiol Hung. 2009 Jun; 96(2):231-41.AP

Abstract

The aim of the present study was to investigate whether co-administration of nerve growth factor (NGF) and butyrate regulates vanilloid receptor 1 (VR1) and substance P (SP) levels in cultures of rat dorsal root ganglion (DRG) neurons. DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociated cells for 2 days then exposed to NGF (10 ng/ml), butyrate (1 mmol/L), NGF (10 ng/ml) plus butyrate (1 mmol/L) for another 4 days. The neurons cultured continuously in media served as normal control. After that, the cultures were processed for detecting expression of mRNA for VR1 and SP in DRG neurons by RT-PCR, and expression of VR1 protein by Western blot. SP basal release levels were measured by radioimmunoassay (RIA). Capsaicin-evoked SP release was measured by RIA after stimulation with capsaicin (100 nmol/L) for 10 minutes. The neurons exposed to vehicle solution served as vehicle control. Either NGF (10 ng/ml) or butyrate (1 mmol/L) promoted expression of SP mRNA, VR1 mRNA, and VR1 protein in DRG neurons and capsaicin-evoked SP release from DRG neurons. Co-administration of NGF and butyrate showed a synergistic effect on expression of VR1 mRNA, and VR1 protein in DRG neurons and capsaicin-evoked SP release from DRG neurons and a ceiling effect on SP mRNA expression. The elevation of SP mRNA, VR1 mRNA, and VR1 protein promoted by NGF and/or butyrate may be associated with increases of SP release evoked by capsaicin. The mechanisms of the effects of co-administration of NGF and butyrate should be clarified by further study.

Authors+Show Affiliations

Shandong University School of Medicine, Department of Anatomy, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19457767

Citation

Liu, Z, et al. "Co-administration of Nerve Growth Factor and Butyrate Regulates Vanilloid Receptor 1 and Substance P Levels in Cultures of Rat Dorsal Root Ganglion Neurons." Acta Physiologica Hungarica, vol. 96, no. 2, 2009, pp. 231-41.
Liu Z, Zhang F, Liu H, et al. Co-administration of nerve growth factor and butyrate regulates vanilloid receptor 1 and substance P levels in cultures of rat dorsal root ganglion neurons. Acta Physiol Hung. 2009;96(2):231-41.
Liu, Z., Zhang, F., Liu, H., Yang, X., Wang, H., & Li, Z. (2009). Co-administration of nerve growth factor and butyrate regulates vanilloid receptor 1 and substance P levels in cultures of rat dorsal root ganglion neurons. Acta Physiologica Hungarica, 96(2), 231-41. https://doi.org/10.1556/APhysiol.96.2009.2.8
Liu Z, et al. Co-administration of Nerve Growth Factor and Butyrate Regulates Vanilloid Receptor 1 and Substance P Levels in Cultures of Rat Dorsal Root Ganglion Neurons. Acta Physiol Hung. 2009;96(2):231-41. PubMed PMID: 19457767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-administration of nerve growth factor and butyrate regulates vanilloid receptor 1 and substance P levels in cultures of rat dorsal root ganglion neurons. AU - Liu,Z, AU - Zhang,F, AU - Liu,H, AU - Yang,X, AU - Wang,H, AU - Li,Zhenzhong, PY - 2009/5/22/entrez PY - 2009/5/22/pubmed PY - 2009/7/1/medline SP - 231 EP - 41 JF - Acta physiologica Hungarica JO - Acta Physiol Hung VL - 96 IS - 2 N2 - The aim of the present study was to investigate whether co-administration of nerve growth factor (NGF) and butyrate regulates vanilloid receptor 1 (VR1) and substance P (SP) levels in cultures of rat dorsal root ganglion (DRG) neurons. DRG was dissected out from embryonic 15-day-old Wistar rat and cultured as dissociated cells for 2 days then exposed to NGF (10 ng/ml), butyrate (1 mmol/L), NGF (10 ng/ml) plus butyrate (1 mmol/L) for another 4 days. The neurons cultured continuously in media served as normal control. After that, the cultures were processed for detecting expression of mRNA for VR1 and SP in DRG neurons by RT-PCR, and expression of VR1 protein by Western blot. SP basal release levels were measured by radioimmunoassay (RIA). Capsaicin-evoked SP release was measured by RIA after stimulation with capsaicin (100 nmol/L) for 10 minutes. The neurons exposed to vehicle solution served as vehicle control. Either NGF (10 ng/ml) or butyrate (1 mmol/L) promoted expression of SP mRNA, VR1 mRNA, and VR1 protein in DRG neurons and capsaicin-evoked SP release from DRG neurons. Co-administration of NGF and butyrate showed a synergistic effect on expression of VR1 mRNA, and VR1 protein in DRG neurons and capsaicin-evoked SP release from DRG neurons and a ceiling effect on SP mRNA expression. The elevation of SP mRNA, VR1 mRNA, and VR1 protein promoted by NGF and/or butyrate may be associated with increases of SP release evoked by capsaicin. The mechanisms of the effects of co-administration of NGF and butyrate should be clarified by further study. SN - 0231-424X UR - https://www.unboundmedicine.com/medline/citation/19457767/Co_administration_of_nerve_growth_factor_and_butyrate_regulates_vanilloid_receptor_1_and_substance_P_levels_in_cultures_of_rat_dorsal_root_ganglion_neurons_ L2 - https://akjournals.com/doi/10.1556/APhysiol.96.2009.2.8 DB - PRIME DP - Unbound Medicine ER -