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Complex antibody profile changes in an experimental autoimmune glaucoma animal model.
Invest Ophthalmol Vis Sci. 2009 Oct; 50(10):4734-42.IO

Abstract

PURPOSE

Increased serum antibodies against heat shock protein 27 (HSP27) have been identified in patients with glaucoma. Immunization with HSP27 caused retinal ganglion cell (RGC) loss in animals. The authors analyzed whether HSP27 immunization not only causes RGC loss but also affects systemic antibody patterns.

METHODS

Rats were immunized with HSP27 and were surveyed for 4, 5, and 6 weeks (groups 1-3). Control animals were humanely killed after 6 weeks (group 4). Intraocular pressure was measured before and 2 and 4 weeks after immunization. Fundus images were taken at the same time. Retinal flatmounts were prepared, and Brn-3a labeled RGCs were counted. Serum was collected during the study to detect antibody patterns against retinal antigens through Western blot analysis and mass spectrometry techniques. Patterns were analyzed by multivariate statistical techniques, and biomarkers were identified with the use of mass spectrometry.

RESULTS

No significant changes in intraocular pressure were observed, and no fundus abnormalities were noted. The animals immunized with HSP27 showed lower RGC density than controls (P < 0.05). Two and 4 weeks after immunization, we detected a significant difference in antibody profiles between groups 1 and 4 (P < 0.05) and groups 3 and 4 (P < 0.05). Proteins with different antibody level expression after immunization included heat shock protein 90, alpha-enolase, and glyceraldehyde-3-phosphate dehydrogenase.

CONCLUSIONS

After immunization with HSP27, animals showed IOP-independent RGC loss and changes in serum antibody patterns. Thus, this model might be a beneficial approach to study the development and effects of anti-retinal antibodies and their involvement in RGC loss.

Authors+Show Affiliations

Experimental Ophthalmology, Department of Ophthalmology, Johannes Gutenberg University, Mainz, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19458332

Citation

Joachim, Stephanie C., et al. "Complex Antibody Profile Changes in an Experimental Autoimmune Glaucoma Animal Model." Investigative Ophthalmology & Visual Science, vol. 50, no. 10, 2009, pp. 4734-42.
Joachim SC, Grus FH, Kraft D, et al. Complex antibody profile changes in an experimental autoimmune glaucoma animal model. Invest Ophthalmol Vis Sci. 2009;50(10):4734-42.
Joachim, S. C., Grus, F. H., Kraft, D., White-Farrar, K., Barnes, G., Barbeck, M., Ghanaati, S., Cao, S., Li, B., & Wax, M. B. (2009). Complex antibody profile changes in an experimental autoimmune glaucoma animal model. Investigative Ophthalmology & Visual Science, 50(10), 4734-42. https://doi.org/10.1167/iovs.08-3144
Joachim SC, et al. Complex Antibody Profile Changes in an Experimental Autoimmune Glaucoma Animal Model. Invest Ophthalmol Vis Sci. 2009;50(10):4734-42. PubMed PMID: 19458332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complex antibody profile changes in an experimental autoimmune glaucoma animal model. AU - Joachim,Stephanie C, AU - Grus,Franz H, AU - Kraft,Daniela, AU - White-Farrar,Kisha, AU - Barnes,George, AU - Barbeck,Mike, AU - Ghanaati,Shahram, AU - Cao,Shutong, AU - Li,Byron, AU - Wax,Martin B, Y1 - 2009/05/20/ PY - 2009/5/22/entrez PY - 2009/5/22/pubmed PY - 2009/10/3/medline SP - 4734 EP - 42 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 50 IS - 10 N2 - PURPOSE: Increased serum antibodies against heat shock protein 27 (HSP27) have been identified in patients with glaucoma. Immunization with HSP27 caused retinal ganglion cell (RGC) loss in animals. The authors analyzed whether HSP27 immunization not only causes RGC loss but also affects systemic antibody patterns. METHODS: Rats were immunized with HSP27 and were surveyed for 4, 5, and 6 weeks (groups 1-3). Control animals were humanely killed after 6 weeks (group 4). Intraocular pressure was measured before and 2 and 4 weeks after immunization. Fundus images were taken at the same time. Retinal flatmounts were prepared, and Brn-3a labeled RGCs were counted. Serum was collected during the study to detect antibody patterns against retinal antigens through Western blot analysis and mass spectrometry techniques. Patterns were analyzed by multivariate statistical techniques, and biomarkers were identified with the use of mass spectrometry. RESULTS: No significant changes in intraocular pressure were observed, and no fundus abnormalities were noted. The animals immunized with HSP27 showed lower RGC density than controls (P < 0.05). Two and 4 weeks after immunization, we detected a significant difference in antibody profiles between groups 1 and 4 (P < 0.05) and groups 3 and 4 (P < 0.05). Proteins with different antibody level expression after immunization included heat shock protein 90, alpha-enolase, and glyceraldehyde-3-phosphate dehydrogenase. CONCLUSIONS: After immunization with HSP27, animals showed IOP-independent RGC loss and changes in serum antibody patterns. Thus, this model might be a beneficial approach to study the development and effects of anti-retinal antibodies and their involvement in RGC loss. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/19458332/Complex_antibody_profile_changes_in_an_experimental_autoimmune_glaucoma_animal_model_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.08-3144 DB - PRIME DP - Unbound Medicine ER -