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Evaluation of antimicrobial and anti-inflammatory activities of seed extracts from six Nigella species.
J Med Food. 2009 Apr; 12(2):408-15.JM

Abstract

Seed extracts from six species of the genus Nigella (Family Ranunculaceae)-Nigella arvensis, Nigella damascena, Nigella hispanica, Nigella nigellastrum, Nigella orientalis, and Nigella sativa-obtained by successive extraction with n-hexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution method as well as for anti-inflammatory properties by in vitro cyclooxygenase (COX)-1 and COX-2 assay. Chemical characterization of active extracts was carried out including free and fixed fatty acid analysis. Comparison of antimicrobial activity showed that N. arvensis chloroform extract was the most potent among all species tested, inhibiting Gram-positive bacterial and yeast strains with minimum inhibitory concentration (MIC) values ranging from 0.25 to 1 mg/mL. With the exception of selective inhibitory action of n-hexane extract of N. orientalis on growth of Bacteroides fragilis (MIC = 0.5 mg/mL), we observed no antimicrobial activity for other Nigella species. Anti-inflammatory screening revealed that N. sativa, N. orientalis, N. hispanica, N. arvensis n-hexane, and N. hispanica chloroform extracts had strong inhibitory activity (more than 80%) on COX-1 and N. orientalis, N. arvensis, and N. hispanica n-hexane extracts were most effective against COX-2, when the concentration of extracts was 100 microg/mL in both COX assays. In conclusion, N. arvensis, N. orientalis, and N. hispanica seeds, for the first time examined for antimicrobial and anti-inflammatory effects, revealed their significant activity in one or both assays.

Authors+Show Affiliations

Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany AS CR, vvi, Czech University of Life Sciences Prague, Prague, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19459745

Citation

Landa, Premysl, et al. "Evaluation of Antimicrobial and Anti-inflammatory Activities of Seed Extracts From Six Nigella Species." Journal of Medicinal Food, vol. 12, no. 2, 2009, pp. 408-15.
Landa P, Marsik P, Havlik J, et al. Evaluation of antimicrobial and anti-inflammatory activities of seed extracts from six Nigella species. J Med Food. 2009;12(2):408-15.
Landa, P., Marsik, P., Havlik, J., Kloucek, P., Vanek, T., & Kokoska, L. (2009). Evaluation of antimicrobial and anti-inflammatory activities of seed extracts from six Nigella species. Journal of Medicinal Food, 12(2), 408-15. https://doi.org/10.1089/jmf.2007.0600
Landa P, et al. Evaluation of Antimicrobial and Anti-inflammatory Activities of Seed Extracts From Six Nigella Species. J Med Food. 2009;12(2):408-15. PubMed PMID: 19459745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antimicrobial and anti-inflammatory activities of seed extracts from six Nigella species. AU - Landa,Premysl, AU - Marsik,Petr, AU - Havlik,Jaroslav, AU - Kloucek,Pavel, AU - Vanek,Tomas, AU - Kokoska,Ladislav, PY - 2009/5/23/entrez PY - 2009/5/23/pubmed PY - 2009/9/11/medline SP - 408 EP - 15 JF - Journal of medicinal food JO - J Med Food VL - 12 IS - 2 N2 - Seed extracts from six species of the genus Nigella (Family Ranunculaceae)-Nigella arvensis, Nigella damascena, Nigella hispanica, Nigella nigellastrum, Nigella orientalis, and Nigella sativa-obtained by successive extraction with n-hexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution method as well as for anti-inflammatory properties by in vitro cyclooxygenase (COX)-1 and COX-2 assay. Chemical characterization of active extracts was carried out including free and fixed fatty acid analysis. Comparison of antimicrobial activity showed that N. arvensis chloroform extract was the most potent among all species tested, inhibiting Gram-positive bacterial and yeast strains with minimum inhibitory concentration (MIC) values ranging from 0.25 to 1 mg/mL. With the exception of selective inhibitory action of n-hexane extract of N. orientalis on growth of Bacteroides fragilis (MIC = 0.5 mg/mL), we observed no antimicrobial activity for other Nigella species. Anti-inflammatory screening revealed that N. sativa, N. orientalis, N. hispanica, N. arvensis n-hexane, and N. hispanica chloroform extracts had strong inhibitory activity (more than 80%) on COX-1 and N. orientalis, N. arvensis, and N. hispanica n-hexane extracts were most effective against COX-2, when the concentration of extracts was 100 microg/mL in both COX assays. In conclusion, N. arvensis, N. orientalis, and N. hispanica seeds, for the first time examined for antimicrobial and anti-inflammatory effects, revealed their significant activity in one or both assays. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/19459745/Evaluation_of_antimicrobial_and_anti_inflammatory_activities_of_seed_extracts_from_six_Nigella_species_ L2 - https://www.liebertpub.com/doi/10.1089/jmf.2007.0600?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -