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Isolation and characterization of a protective bacterial culture isolated from honey active against American Foulbrood disease.
FEMS Microbiol Lett. 2009 Jul; 296(1):39-44.FM

Abstract

Bacterial strains isolated from US domestic honey were screened for antibacterial activity against Paenibacillus larvae ssp. larvae, the causative agent of American Foulbrood (AFB) in apiaries. A bacterial isolate (TH13) showing a high level of antimicrobial activity against P. larvae ssp. larvae ATCC 9545 was selected and identified as Paenibacillus polymyxa by 16S rRNA gene sequencing. The antimicrobial compound was purified by 80% saturated ammonium sulfate precipitation followed by CM-sepharose chromatography and reverse-phase HPLC. The molecular mass of the compound was determined to be 1168.78 Da by ESI-qTOF MS, matching that of polymyxin E1. The producer strain showed a broad range of antibacterial activity against gram-positive and -negative bacteria including P. larvae ssp. larvae ATCC 25747 and foodborne pathogens such as Bacillus cereus F4552 and Escherichia coli O157:H7 ATCC 43895. The selection of antibiotic-producing bacterial strains indigenous to honey as protective cultures against AFB may lessen the use of antibiotics in apiaries.

Authors+Show Affiliations

Department of Food Science and Technology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19459943

Citation

Lee, Hyungjae, et al. "Isolation and Characterization of a Protective Bacterial Culture Isolated From Honey Active Against American Foulbrood Disease." FEMS Microbiology Letters, vol. 296, no. 1, 2009, pp. 39-44.
Lee H, Churey JJ, Worobo RW. Isolation and characterization of a protective bacterial culture isolated from honey active against American Foulbrood disease. FEMS Microbiol Lett. 2009;296(1):39-44.
Lee, H., Churey, J. J., & Worobo, R. W. (2009). Isolation and characterization of a protective bacterial culture isolated from honey active against American Foulbrood disease. FEMS Microbiology Letters, 296(1), 39-44. https://doi.org/10.1111/j.1574-6968.2009.01615.x
Lee H, Churey JJ, Worobo RW. Isolation and Characterization of a Protective Bacterial Culture Isolated From Honey Active Against American Foulbrood Disease. FEMS Microbiol Lett. 2009;296(1):39-44. PubMed PMID: 19459943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of a protective bacterial culture isolated from honey active against American Foulbrood disease. AU - Lee,Hyungjae, AU - Churey,John J, AU - Worobo,Randy W, Y1 - 2009/04/15/ PY - 2009/5/23/entrez PY - 2009/5/23/pubmed PY - 2009/9/22/medline SP - 39 EP - 44 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 296 IS - 1 N2 - Bacterial strains isolated from US domestic honey were screened for antibacterial activity against Paenibacillus larvae ssp. larvae, the causative agent of American Foulbrood (AFB) in apiaries. A bacterial isolate (TH13) showing a high level of antimicrobial activity against P. larvae ssp. larvae ATCC 9545 was selected and identified as Paenibacillus polymyxa by 16S rRNA gene sequencing. The antimicrobial compound was purified by 80% saturated ammonium sulfate precipitation followed by CM-sepharose chromatography and reverse-phase HPLC. The molecular mass of the compound was determined to be 1168.78 Da by ESI-qTOF MS, matching that of polymyxin E1. The producer strain showed a broad range of antibacterial activity against gram-positive and -negative bacteria including P. larvae ssp. larvae ATCC 25747 and foodborne pathogens such as Bacillus cereus F4552 and Escherichia coli O157:H7 ATCC 43895. The selection of antibiotic-producing bacterial strains indigenous to honey as protective cultures against AFB may lessen the use of antibiotics in apiaries. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/19459943/Isolation_and_characterization_of_a_protective_bacterial_culture_isolated_from_honey_active_against_American_Foulbrood_disease_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2009.01615.x DB - PRIME DP - Unbound Medicine ER -