Tags

Type your tag names separated by a space and hit enter

Fingerprinting analysis of Oenococcus oeni strains under stress conditions.
FEMS Microbiol Lett 2009; 296(1):11-7FM

Abstract

Oenococcus oeni strains from traditional Italian red wines of the Basilicata region were investigated on the basis of their physiological and molecular response to different temperatures and ethanol concentrations. All strains were highly resistant to different ethanol concentrations and it has been observed that 7% ethanol was able to stimulate the growth of strains in wine, and 12-13% of ethanol allowed their proliferation. Moreover, strain tolerances to 18 and 42 degrees C were observed. Fingerprinting analysis with fluorescent differential display-PCR and investigation of changes in gene expression during the tolerance process were carried out. The expression gene pattern reflects mechanisms involved in tolerance to environmental conditions. This study establishes and validates a method that enables, with a high reproducibility, different gene expression identification under stress conditions in lactic acid bacteria.

Authors+Show Affiliations

Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Potenza, Italy. mariaanna.sico@unibas.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19459945

Citation

Sico, Maria Anna, et al. "Fingerprinting Analysis of Oenococcus Oeni Strains Under Stress Conditions." FEMS Microbiology Letters, vol. 296, no. 1, 2009, pp. 11-7.
Sico MA, Bonomo MG, D'Adamo A, et al. Fingerprinting analysis of Oenococcus oeni strains under stress conditions. FEMS Microbiol Lett. 2009;296(1):11-7.
Sico, M. A., Bonomo, M. G., D'Adamo, A., Bochicchio, S., & Salzano, G. (2009). Fingerprinting analysis of Oenococcus oeni strains under stress conditions. FEMS Microbiology Letters, 296(1), pp. 11-7. doi:10.1111/j.1574-6968.2009.01611.x.
Sico MA, et al. Fingerprinting Analysis of Oenococcus Oeni Strains Under Stress Conditions. FEMS Microbiol Lett. 2009;296(1):11-7. PubMed PMID: 19459945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fingerprinting analysis of Oenococcus oeni strains under stress conditions. AU - Sico,Maria Anna, AU - Bonomo,Maria Grazia, AU - D'Adamo,Alba, AU - Bochicchio,Sabrina, AU - Salzano,Giovanni, Y1 - 2009/05/15/ PY - 2009/5/23/entrez PY - 2009/5/23/pubmed PY - 2009/9/22/medline SP - 11 EP - 7 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 296 IS - 1 N2 - Oenococcus oeni strains from traditional Italian red wines of the Basilicata region were investigated on the basis of their physiological and molecular response to different temperatures and ethanol concentrations. All strains were highly resistant to different ethanol concentrations and it has been observed that 7% ethanol was able to stimulate the growth of strains in wine, and 12-13% of ethanol allowed their proliferation. Moreover, strain tolerances to 18 and 42 degrees C were observed. Fingerprinting analysis with fluorescent differential display-PCR and investigation of changes in gene expression during the tolerance process were carried out. The expression gene pattern reflects mechanisms involved in tolerance to environmental conditions. This study establishes and validates a method that enables, with a high reproducibility, different gene expression identification under stress conditions in lactic acid bacteria. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/19459945/Fingerprinting_analysis_of_Oenococcus_oeni_strains_under_stress_conditions_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2009.01611.x DB - PRIME DP - Unbound Medicine ER -