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[Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on its expression in mice with obstructive nephropathy].
Nan Fang Yi Ke Da Xue Xue Bao. 2009 May; 29(5):909-13.NF

Abstract

OBJECTIVE

To investigate the role of integrin-linked kinase (ILK) on renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on LIK expression in mice with obstructive nephropathy.

METHODS

Normal male mice were randomly divided into sham-operated group (n=20), unilateral ureteral obstruction (UUO) group (n=28), and UUO with urokinase treatment group (uPA, n=28), and UUO was induced surgically in the latter two groups. The mice were sacrificed on days l, 3, 7 and 14 after the surgery, and renal interstitial fibrosis (RIF) was graded according to the result of Masson staining. The expression of ILK in the renal tissues of the rats was examined by immunofluorescence staining and Western blotting, and the expression of E-cadherin was detected by immunohistochemistry. RT-PCR was used to examine the mRNA expressions of ILK, E-cadherin and alpha-smooth muscle actin (alpha-SMA).

RESULTS

The expressions of ILK mRNA and protein were significantly increased in UUO group, but significantly decreased by treatment with uPA (P<0.05). The expression of alpha-SMA mRNA level was significantly increased, while E-cadherin decreased in mice with UUO on day 3 after the surgery. Treatment with uPA significantly inhibited such effects (P<0.05).

CONCLUSION

ILK plays an important role in renal interstitial fibrosis by mediating epithelial-mesenchymal transition. Urokinase attenuates renal tubulointerstitial fibrosis in mice with UUO possibly by inhibiting ILK expression and preventing tubular epithelial-mesenchymal transition.

Authors+Show Affiliations

Graduate College of Southern Medical University, Department of Nephrology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510515, China. hefengmm@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19460706

Citation

He, Feng, et al. "[Role of Integrin-linked Kinase in Renal Tubular Epithelial-mesenchymal Transition and the Regulatory Effect of Urokinase On Its Expression in Mice With Obstructive Nephropathy]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 29, no. 5, 2009, pp. 909-13.
He F, Yu L, Tong JR, et al. [Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on its expression in mice with obstructive nephropathy]. Nan Fang Yi Ke Da Xue Xue Bao. 2009;29(5):909-13.
He, F., Yu, L., Tong, J. R., Luo, Z. M., Zhu, Q. Z., Wang, Y., & Zhang, J. L. (2009). [Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on its expression in mice with obstructive nephropathy]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 29(5), 909-13.
He F, et al. [Role of Integrin-linked Kinase in Renal Tubular Epithelial-mesenchymal Transition and the Regulatory Effect of Urokinase On Its Expression in Mice With Obstructive Nephropathy]. Nan Fang Yi Ke Da Xue Xue Bao. 2009;29(5):909-13. PubMed PMID: 19460706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Role of integrin-linked kinase in renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on its expression in mice with obstructive nephropathy]. AU - He,Feng, AU - Yu,Lu, AU - Tong,Jun-rong, AU - Luo,Zheng-mao, AU - Zhu,Qi-zhi, AU - Wang,Yin, AU - Zhang,Jian-lin, PY - 2009/5/23/entrez PY - 2009/5/23/pubmed PY - 2010/5/5/medline SP - 909 EP - 13 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 29 IS - 5 N2 - OBJECTIVE: To investigate the role of integrin-linked kinase (ILK) on renal tubular epithelial-mesenchymal transition and the regulatory effect of urokinase on LIK expression in mice with obstructive nephropathy. METHODS: Normal male mice were randomly divided into sham-operated group (n=20), unilateral ureteral obstruction (UUO) group (n=28), and UUO with urokinase treatment group (uPA, n=28), and UUO was induced surgically in the latter two groups. The mice were sacrificed on days l, 3, 7 and 14 after the surgery, and renal interstitial fibrosis (RIF) was graded according to the result of Masson staining. The expression of ILK in the renal tissues of the rats was examined by immunofluorescence staining and Western blotting, and the expression of E-cadherin was detected by immunohistochemistry. RT-PCR was used to examine the mRNA expressions of ILK, E-cadherin and alpha-smooth muscle actin (alpha-SMA). RESULTS: The expressions of ILK mRNA and protein were significantly increased in UUO group, but significantly decreased by treatment with uPA (P<0.05). The expression of alpha-SMA mRNA level was significantly increased, while E-cadherin decreased in mice with UUO on day 3 after the surgery. Treatment with uPA significantly inhibited such effects (P<0.05). CONCLUSION: ILK plays an important role in renal interstitial fibrosis by mediating epithelial-mesenchymal transition. Urokinase attenuates renal tubulointerstitial fibrosis in mice with UUO possibly by inhibiting ILK expression and preventing tubular epithelial-mesenchymal transition. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/19460706/[Role_of_integrin_linked_kinase_in_renal_tubular_epithelial_mesenchymal_transition_and_the_regulatory_effect_of_urokinase_on_its_expression_in_mice_with_obstructive_nephropathy]_ L2 - https://antibodies.cancer.gov/detail/CPTC-CDH1-3 DB - PRIME DP - Unbound Medicine ER -