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[Mesenchymal stem cells transplanted in mdx mice differentiate into myocytes and express dystrophin/utrophin].
Nan Fang Yi Ke Da Xue Xue Bao. 2009 May; 29(5):974-8.NF

Abstract

OBJECTIVE

To investigate the differentiation of rat bone marrow mesenchymal stem cells (MSCs) into myocytes and their expression of dystrophin/utrophin after transplantation in mdx mice.

METHODS

BrdU-labeled fifth-passage rat MSCs were transplanted in mdx mice with previous total body gamma irradiation (7 Gy). At 4, 8, 12 and 16 weeks after the transplantation, the mice were sacrificed to detect dystrophin/BrdU and utrophin expressions in the gastrocnemius muscle using immunofluorescence assay, RT-PCR and Western blotting. Five normal C57 BL/6 mice and 5 mdx mice served as the positive and negative controls, respectively.

RESULTS

Four weeks after MSC transplantation, less than 1% of the muscle fibers of the mdx mice expressed dystrophin, which increased to 15% at 16 weeks. Donor-derived nuclei were detected in both single and clusters of dystrophin-positive fibers. Some BrdU-positive nuclei were centrally located, and some peripherally within myofibers. Utrophin expression decreased over time after transplantation.

CONCLUSION

The myofibers of mdx mice with MSC transplantation express dystrophin, which is derived partially from the transplanted MSCs. Dystrophin expression from the transplanted MSCs partially inhibits the upregulation of utrophin in mdx mouse muscle, showing a complementary relation between them.

Authors+Show Affiliations

Feng SW
Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Center for Stem Cell Biology and Tissue Engineering, Guangzhou 510080, China.
Zhang C
No affiliation info available
Lu XL
No affiliation info available
Liu TY
No affiliation info available
Li CM
No affiliation info available
Yao XL
No affiliation info available
Yu MJ
No affiliation info available

MeSH

AnimalsBone Marrow CellsCell DifferentiationDystrophinMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMiceMice, Inbred C57BLMice, Inbred mdxMuscle Fibers, SkeletalMuscular Dystrophy, AnimalRatsUtrophin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19460724

Citation

Feng, Shan-wei, et al. "[Mesenchymal Stem Cells Transplanted in Mdx Mice Differentiate Into Myocytes and Express Dystrophin/utrophin]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 29, no. 5, 2009, pp. 974-8.
Feng SW, Zhang C, Lu XL, et al. [Mesenchymal stem cells transplanted in mdx mice differentiate into myocytes and express dystrophin/utrophin]. Nan Fang Yi Ke Da Xue Xue Bao. 2009;29(5):974-8.
Feng, S. W., Zhang, C., Lu, X. L., Liu, T. Y., Li, C. M., Yao, X. L., & Yu, M. J. (2009). [Mesenchymal stem cells transplanted in mdx mice differentiate into myocytes and express dystrophin/utrophin]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 29(5), 974-8.
Feng SW, et al. [Mesenchymal Stem Cells Transplanted in Mdx Mice Differentiate Into Myocytes and Express Dystrophin/utrophin]. Nan Fang Yi Ke Da Xue Xue Bao. 2009;29(5):974-8. PubMed PMID: 19460724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mesenchymal stem cells transplanted in mdx mice differentiate into myocytes and express dystrophin/utrophin]. AU - Feng,Shan-wei, AU - Zhang,Cheng, AU - Lu,Xi-lin, AU - Liu,Tai-yun, AU - Li,Cai-ming, AU - Yao,Xiao-li, AU - Yu,Mei-juan, PY - 2009/5/23/entrez PY - 2009/5/23/pubmed PY - 2010/5/5/medline SP - 974 EP - 8 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 29 IS - 5 N2 - OBJECTIVE: To investigate the differentiation of rat bone marrow mesenchymal stem cells (MSCs) into myocytes and their expression of dystrophin/utrophin after transplantation in mdx mice. METHODS: BrdU-labeled fifth-passage rat MSCs were transplanted in mdx mice with previous total body gamma irradiation (7 Gy). At 4, 8, 12 and 16 weeks after the transplantation, the mice were sacrificed to detect dystrophin/BrdU and utrophin expressions in the gastrocnemius muscle using immunofluorescence assay, RT-PCR and Western blotting. Five normal C57 BL/6 mice and 5 mdx mice served as the positive and negative controls, respectively. RESULTS: Four weeks after MSC transplantation, less than 1% of the muscle fibers of the mdx mice expressed dystrophin, which increased to 15% at 16 weeks. Donor-derived nuclei were detected in both single and clusters of dystrophin-positive fibers. Some BrdU-positive nuclei were centrally located, and some peripherally within myofibers. Utrophin expression decreased over time after transplantation. CONCLUSION: The myofibers of mdx mice with MSC transplantation express dystrophin, which is derived partially from the transplanted MSCs. Dystrophin expression from the transplanted MSCs partially inhibits the upregulation of utrophin in mdx mouse muscle, showing a complementary relation between them. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/19460724/[Mesenchymal_stem_cells_transplanted_in_mdx_mice_differentiate_into_myocytes_and_express_dystrophin/utrophin]_ DB - PRIME DP - Unbound Medicine ER -