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Dietary soy isoflavone replacement improves detrusor overactivity of ovariectomized rats with altered connexin-43 expression in the urinary bladder.
BJU Int. 2009 May; 103(10):1429-35.BI

Abstract

To evaluate the effects of dietary soy isoflavone on detrusor overactivity (DO) in ovariectomized (Ovx) rats and the association between these effects and expression of the gap junction protein, connexin-43, in the urinary bladder, and to discuss the usefulness of soy isoflavones for overactive bladder (OAB).

MATERIALS AND METHODS

In all, 24 (8-week-old) virgin female Sprague-Dawley rats were divided equally into four groups: sham operation with control diet (with no dietary soy isoflavones; Sham-CD), bilateral Ovx with CD (Ovx-CD), sham operation with soy isoflavone diet (Sham-ID), bilateral Ovx with soy ID (Ovx-ID). Cystometry was performed after the 4-week CD or ID in each group while awake. Quantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical and Western blot analyses were also performed to examine the expression of connexin-43 in each group.

RESULTS

In Ovx-CD rats, there were some significant changes in cystometry variables, including shortening of the intercontraction interval and an increased number of non-voiding contractions compared with Sham-CD rats (P < 0.05). These changes were significantly improved by 4-week soy isoflavone administration (Ovx-ID; P < 0.05). Quantitative RT-PCR, immunohistochemical and Western blot analyses showed that the expression level of connexin-43 mRNA and protein was significantly greater in the urinary bladder of Ovx-CD rats compared with Sham-CD rats. Soy isoflavone administration significantly reduced this increased expression (Ovx-ID).

CONCLUSIONS

Soy isoflavone replacement improved DO with alteration of the connexin-43 expression pattern in the urinary bladder of Ovx rats. Routine consumption of diet soy isoflavones may be a useful treatment to prevent and improve OAB.

Authors+Show Affiliations

Department of Nephro-urology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19462485

Citation

Okada, Shinsuke, et al. "Dietary Soy Isoflavone Replacement Improves Detrusor Overactivity of Ovariectomized Rats With Altered Connexin-43 Expression in the Urinary Bladder." BJU International, vol. 103, no. 10, 2009, pp. 1429-35.
Okada S, Kojima Y, Hamamoto S, et al. Dietary soy isoflavone replacement improves detrusor overactivity of ovariectomized rats with altered connexin-43 expression in the urinary bladder. BJU Int. 2009;103(10):1429-35.
Okada, S., Kojima, Y., Hamamoto, S., Mizuno, K., Sasaki, S., & Kohri, K. (2009). Dietary soy isoflavone replacement improves detrusor overactivity of ovariectomized rats with altered connexin-43 expression in the urinary bladder. BJU International, 103(10), 1429-35.
Okada S, et al. Dietary Soy Isoflavone Replacement Improves Detrusor Overactivity of Ovariectomized Rats With Altered Connexin-43 Expression in the Urinary Bladder. BJU Int. 2009;103(10):1429-35. PubMed PMID: 19462485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary soy isoflavone replacement improves detrusor overactivity of ovariectomized rats with altered connexin-43 expression in the urinary bladder. AU - Okada,Shinsuke, AU - Kojima,Yoshiyuki, AU - Hamamoto,Shuzo, AU - Mizuno,Kentaro, AU - Sasaki,Shoichi, AU - Kohri,Kenjiro, PY - 2009/5/23/entrez PY - 2009/5/23/pubmed PY - 2009/6/2/medline SP - 1429 EP - 35 JF - BJU international JO - BJU Int. VL - 103 IS - 10 N2 - UNLABELLED: To evaluate the effects of dietary soy isoflavone on detrusor overactivity (DO) in ovariectomized (Ovx) rats and the association between these effects and expression of the gap junction protein, connexin-43, in the urinary bladder, and to discuss the usefulness of soy isoflavones for overactive bladder (OAB). MATERIALS AND METHODS: In all, 24 (8-week-old) virgin female Sprague-Dawley rats were divided equally into four groups: sham operation with control diet (with no dietary soy isoflavones; Sham-CD), bilateral Ovx with CD (Ovx-CD), sham operation with soy isoflavone diet (Sham-ID), bilateral Ovx with soy ID (Ovx-ID). Cystometry was performed after the 4-week CD or ID in each group while awake. Quantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical and Western blot analyses were also performed to examine the expression of connexin-43 in each group. RESULTS: In Ovx-CD rats, there were some significant changes in cystometry variables, including shortening of the intercontraction interval and an increased number of non-voiding contractions compared with Sham-CD rats (P < 0.05). These changes were significantly improved by 4-week soy isoflavone administration (Ovx-ID; P < 0.05). Quantitative RT-PCR, immunohistochemical and Western blot analyses showed that the expression level of connexin-43 mRNA and protein was significantly greater in the urinary bladder of Ovx-CD rats compared with Sham-CD rats. Soy isoflavone administration significantly reduced this increased expression (Ovx-ID). CONCLUSIONS: Soy isoflavone replacement improved DO with alteration of the connexin-43 expression pattern in the urinary bladder of Ovx rats. Routine consumption of diet soy isoflavones may be a useful treatment to prevent and improve OAB. SN - 1464-410X UR - https://www.unboundmedicine.com/medline/citation/19462485/Dietary_soy_isoflavone_replacement_improves_detrusor_overactivity_of_ovariectomized_rats_with_altered_connexin_43_expression_in_the_urinary_bladder_ L2 - https://doi.org/10.1111/j.1464-410x.2008.08325.x DB - PRIME DP - Unbound Medicine ER -