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Expression and characterization of jingzhaotoxin-34, a novel neurotoxin from the venom of the tarantula Chilobrachys jingzhao.
Peptides. 2009 Jun; 30(6):1042-8.P

Abstract

Jingzhaotoxin-34 (JZTX-34) is a 35-residue polypeptide from the venom of Chinese tarantula Chilobrachys jingzhao. Our previous work reported its full-length cDNA sequence encoding a precursor with 87 residues. In this study we report the protein expression and biological function characterization. The toxin was efficiently expressed by the secretary pathway in yeast. Under whole-cell patch-clamp mode, the expressed JZTX-34 was able to inhibit tetrodotoxin-sensitive (TTX-S) sodium currents (IC(50) approximately 85 nM) while having no significant effects on tetrodotoxin-resistant (TTX-R) sodium currents on rat dorsal root ganglion neurons. The inhibition of TTX-S sodium channels was completely reversed by strong depolarization (+120 mV). Toxin treatment altered neither channel activation and inactivation kinetics nor recovery rate from inactivation. However, it is interesting to note that in contrast to huwentoxin-IV, a recently identified receptor site-4 toxin from Ornithoctonus huwena venom, 100 nM JZTX-34 caused a negative shift of steady-state inactivation curve of TTX-S sodium channels by approximately 10 mV. The results indicated that JZTX-34 might inhibit mammalian sensory neuronal sodium channels through a mechanism similar to HWTX-IV by trapping the IIS4 voltage sensor in the resting conformation, but their binding sites should not overlay completely.

Authors+Show Affiliations

The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19463735

Citation

Chen, Jinjun, et al. "Expression and Characterization of Jingzhaotoxin-34, a Novel Neurotoxin From the Venom of the Tarantula Chilobrachys Jingzhao." Peptides, vol. 30, no. 6, 2009, pp. 1042-8.
Chen J, Zhang Y, Rong M, et al. Expression and characterization of jingzhaotoxin-34, a novel neurotoxin from the venom of the tarantula Chilobrachys jingzhao. Peptides. 2009;30(6):1042-8.
Chen, J., Zhang, Y., Rong, M., Zhao, L., Jiang, L., Zhang, D., Wang, M., Xiao, Y., & Liang, S. (2009). Expression and characterization of jingzhaotoxin-34, a novel neurotoxin from the venom of the tarantula Chilobrachys jingzhao. Peptides, 30(6), 1042-8. https://doi.org/10.1016/j.peptides.2009.02.018
Chen J, et al. Expression and Characterization of Jingzhaotoxin-34, a Novel Neurotoxin From the Venom of the Tarantula Chilobrachys Jingzhao. Peptides. 2009;30(6):1042-8. PubMed PMID: 19463735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and characterization of jingzhaotoxin-34, a novel neurotoxin from the venom of the tarantula Chilobrachys jingzhao. AU - Chen,Jinjun, AU - Zhang,Yongqun, AU - Rong,Mingqiang, AU - Zhao,Liqun, AU - Jiang,Liping, AU - Zhang,Dongyi, AU - Wang,Meichi, AU - Xiao,Yucheng, AU - Liang,Songping, Y1 - 2009/03/13/ PY - 2009/01/17/received PY - 2009/02/23/revised PY - 2009/02/26/accepted PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/9/2/medline SP - 1042 EP - 8 JF - Peptides JO - Peptides VL - 30 IS - 6 N2 - Jingzhaotoxin-34 (JZTX-34) is a 35-residue polypeptide from the venom of Chinese tarantula Chilobrachys jingzhao. Our previous work reported its full-length cDNA sequence encoding a precursor with 87 residues. In this study we report the protein expression and biological function characterization. The toxin was efficiently expressed by the secretary pathway in yeast. Under whole-cell patch-clamp mode, the expressed JZTX-34 was able to inhibit tetrodotoxin-sensitive (TTX-S) sodium currents (IC(50) approximately 85 nM) while having no significant effects on tetrodotoxin-resistant (TTX-R) sodium currents on rat dorsal root ganglion neurons. The inhibition of TTX-S sodium channels was completely reversed by strong depolarization (+120 mV). Toxin treatment altered neither channel activation and inactivation kinetics nor recovery rate from inactivation. However, it is interesting to note that in contrast to huwentoxin-IV, a recently identified receptor site-4 toxin from Ornithoctonus huwena venom, 100 nM JZTX-34 caused a negative shift of steady-state inactivation curve of TTX-S sodium channels by approximately 10 mV. The results indicated that JZTX-34 might inhibit mammalian sensory neuronal sodium channels through a mechanism similar to HWTX-IV by trapping the IIS4 voltage sensor in the resting conformation, but their binding sites should not overlay completely. SN - 1873-5169 UR - https://www.unboundmedicine.com/medline/citation/19463735/Expression_and_characterization_of_jingzhaotoxin_34_a_novel_neurotoxin_from_the_venom_of_the_tarantula_Chilobrachys_jingzhao_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(09)00081-3 DB - PRIME DP - Unbound Medicine ER -