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Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD.
Dev Biol. 2009 Aug 01; 332(1):131-41.DB

Abstract

Satellite cells are tissue-specific stem cells responsible for skeletal muscle growth and regeneration. Although satellite cells were identified almost 50 years ago, the identity of progenitor populations from which they derive remains controversial. We developed MyoD(iCre) knockin mice, and used Cre/lox lineage analysis to determine whether satellite cell progenitors express MyoD, a marker of myogenic commitment. Recombination status of satellite cells was determined by confocal microscopy of isolated muscle fibers and by electron microscopic observation of muscle tissue fixed immediately following isolation, using R26R-EYFP and R26R (beta-gal) reporter mice, respectively. We show that essentially all adult satellite cells associated with limb and body wall musculature, as well as the diaphragm and extraocular muscles, originate from MyoD+ progenitors. Neonatal satellite cells were Cre-recombined, but only a small minority exhibited ongoing Cre expression, indicating that most satellite cells had expressed MyoD prenatally. We also show that satellite cell development in MyoD-null mice is not due to functional compensation by MyoD non-expressing lineages. The results suggest that satellite cells are derived from committed myogenic progenitors, irrespective of the anatomical location, embryological origin, or physiological properties of associated musculature.

Authors+Show Affiliations

Department of Molecular and Cell Biology, Center for Regenerative Biology, University of Connecticut Stem Cell Institute, University of Connecticut, Storrs, CT 06269, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19464281

Citation

Kanisicak, Onur, et al. "Progenitors of Skeletal Muscle Satellite Cells Express the Muscle Determination Gene, MyoD." Developmental Biology, vol. 332, no. 1, 2009, pp. 131-41.
Kanisicak O, Mendez JJ, Yamamoto S, et al. Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD. Dev Biol. 2009;332(1):131-41.
Kanisicak, O., Mendez, J. J., Yamamoto, S., Yamamoto, M., & Goldhamer, D. J. (2009). Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD. Developmental Biology, 332(1), 131-41. https://doi.org/10.1016/j.ydbio.2009.05.554
Kanisicak O, et al. Progenitors of Skeletal Muscle Satellite Cells Express the Muscle Determination Gene, MyoD. Dev Biol. 2009 Aug 1;332(1):131-41. PubMed PMID: 19464281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD. AU - Kanisicak,Onur, AU - Mendez,Julio J, AU - Yamamoto,Shoko, AU - Yamamoto,Masakazu, AU - Goldhamer,David J, Y1 - 2009/05/21/ PY - 2009/01/27/received PY - 2009/05/10/revised PY - 2009/05/15/accepted PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/8/6/medline SP - 131 EP - 41 JF - Developmental biology JO - Dev Biol VL - 332 IS - 1 N2 - Satellite cells are tissue-specific stem cells responsible for skeletal muscle growth and regeneration. Although satellite cells were identified almost 50 years ago, the identity of progenitor populations from which they derive remains controversial. We developed MyoD(iCre) knockin mice, and used Cre/lox lineage analysis to determine whether satellite cell progenitors express MyoD, a marker of myogenic commitment. Recombination status of satellite cells was determined by confocal microscopy of isolated muscle fibers and by electron microscopic observation of muscle tissue fixed immediately following isolation, using R26R-EYFP and R26R (beta-gal) reporter mice, respectively. We show that essentially all adult satellite cells associated with limb and body wall musculature, as well as the diaphragm and extraocular muscles, originate from MyoD+ progenitors. Neonatal satellite cells were Cre-recombined, but only a small minority exhibited ongoing Cre expression, indicating that most satellite cells had expressed MyoD prenatally. We also show that satellite cell development in MyoD-null mice is not due to functional compensation by MyoD non-expressing lineages. The results suggest that satellite cells are derived from committed myogenic progenitors, irrespective of the anatomical location, embryological origin, or physiological properties of associated musculature. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/19464281/Progenitors_of_skeletal_muscle_satellite_cells_express_the_muscle_determination_gene_MyoD_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(09)00873-2 DB - PRIME DP - Unbound Medicine ER -