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Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical hyperalgesia.
Pain. 2009 Aug; 144(3):270-277.PAIN

Abstract

The involvement of TRPV1 and TRPA1 in mediating craniofacial muscle nociception and mechanical hyperalgesia was investigated in male Sprague-Dawley rats. First, we confirmed the expression of TRPV1 in masseter afferents in rat trigeminal ganglia (TG), and provided new data that TRPA1 is also expressed in primary afferents innervating masticatory muscles in double-labeling immunohistochemistry experiments. We then examined whether the activation of each TRP channel in the masseter muscle evokes acute nocifensive responses and leads to the development of masseter hypersensitivity to mechanical stimulation using the behavioral models that have been specifically designed and validated for the craniofacial system. Intramuscular injections with specific agonists for TRPV1 and TRPA1, capsaicin and mustard oil (MO), respectively, produced immediate nocifensive hindpaw responses followed by prolonged mechanical hyperalgesia in a concentration-dependent manner. Pretreatment of the muscle with a TRPV1 antagonist, capsazepine, effectively attenuated the capsaicin-induced muscle nociception and mechanical hyperalgesia. Similarly, pretreatment of the muscle with a selective TRPA1 antagonist, AP18, significantly blocked the MO-induced muscle nociception and mechanical hyperalgesia. We confirmed these data with another set of selective antagonist for TRPV1 and TRPA1, AMG9810 and HC030031, respectively. Collectively, these results provide compelling evidence that TRPV1 and TRPA1 can functionally contribute to muscle nociception and hyperalgesia, and suggest that TRP channels expressed in muscle afferents can engage in the development of pathologic muscle pain conditions.

Authors+Show Affiliations

University of Maryland Baltimore School of Dentistry, Program in Neuroscience, Department of Neural and Pain Sciences, 650 W. Baltimore Street, Baltimore, MD 21201, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19464796

Citation

Ro, Jin Y., et al. "Activation of TRPV1 and TRPA1 Leads to Muscle Nociception and Mechanical Hyperalgesia." Pain, vol. 144, no. 3, 2009, pp. 270-277.
Ro JY, Lee JS, Zhang Y. Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical hyperalgesia. Pain. 2009;144(3):270-277.
Ro, J. Y., Lee, J. S., & Zhang, Y. (2009). Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical hyperalgesia. Pain, 144(3), 270-277. https://doi.org/10.1016/j.pain.2009.04.021
Ro JY, Lee JS, Zhang Y. Activation of TRPV1 and TRPA1 Leads to Muscle Nociception and Mechanical Hyperalgesia. Pain. 2009;144(3):270-277. PubMed PMID: 19464796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical hyperalgesia. AU - Ro,Jin Y, AU - Lee,Jong-Seok, AU - Zhang,Youping, Y1 - 2009/05/22/ PY - 2009/01/08/received PY - 2009/03/26/revised PY - 2009/04/20/accepted PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/10/1/medline SP - 270 EP - 277 JF - Pain JO - Pain VL - 144 IS - 3 N2 - The involvement of TRPV1 and TRPA1 in mediating craniofacial muscle nociception and mechanical hyperalgesia was investigated in male Sprague-Dawley rats. First, we confirmed the expression of TRPV1 in masseter afferents in rat trigeminal ganglia (TG), and provided new data that TRPA1 is also expressed in primary afferents innervating masticatory muscles in double-labeling immunohistochemistry experiments. We then examined whether the activation of each TRP channel in the masseter muscle evokes acute nocifensive responses and leads to the development of masseter hypersensitivity to mechanical stimulation using the behavioral models that have been specifically designed and validated for the craniofacial system. Intramuscular injections with specific agonists for TRPV1 and TRPA1, capsaicin and mustard oil (MO), respectively, produced immediate nocifensive hindpaw responses followed by prolonged mechanical hyperalgesia in a concentration-dependent manner. Pretreatment of the muscle with a TRPV1 antagonist, capsazepine, effectively attenuated the capsaicin-induced muscle nociception and mechanical hyperalgesia. Similarly, pretreatment of the muscle with a selective TRPA1 antagonist, AP18, significantly blocked the MO-induced muscle nociception and mechanical hyperalgesia. We confirmed these data with another set of selective antagonist for TRPV1 and TRPA1, AMG9810 and HC030031, respectively. Collectively, these results provide compelling evidence that TRPV1 and TRPA1 can functionally contribute to muscle nociception and hyperalgesia, and suggest that TRP channels expressed in muscle afferents can engage in the development of pathologic muscle pain conditions. SN - 1872-6623 UR - https://www.unboundmedicine.com/medline/citation/19464796/Activation_of_TRPV1_and_TRPA1_leads_to_muscle_nociception_and_mechanical_hyperalgesia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/00006396-200908000-00011 DB - PRIME DP - Unbound Medicine ER -