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A new variant of Norovirus GII.4/2007 and inter-genotype recombinant strains of NVGII causing acute watery diarrhoea among children in Kolkata, India.
J Clin Virol. 2009 Jul; 45(3):223-9.JC

Abstract

BACKGROUND

Noroviruses (NoVs) are one of the major causal agents of acute gastroenteritis among different age groups. Some of the recent studies reveal that NoV genome is highly prone to mutation and recombination which often leads to emergence of new strains.

OBJECTIVES

To explore the genetic diversity of human Caliciviruses (HuCVs) among diarrhoeic children in Kolkata.

STUDY DESIGN

The HuCVs were detected by reverse transcription-polymerase chain reaction (RT-PCR) of the partial RNA dependent RNA polymerase gene (RdRp) and capsid gene and confirmed by sequencing. The sequences were analyzed and the recombination point was detected.

RESULTS

Faecal specimens of children (n=111) visiting outpatient department of Dr B. C. Roy Memorial Hospital for Children with acute gastroenteritis were studied: 22 cases were HuCV positive with 21 NoVs. Of these, 12 NoV cases (54.5%) were GII.4 and six cases showed 99% identity with the new variant Japanese strain Hu/NoV/GII.4/OC07138/JP. Three novel NoV GII inter-genotype recombinant strains V1628/IND, V1656/IND and V1737/IND were also detected. The RdRp region of V1628 showed 96% identity with Pont de Roide 673/FRN whereas capsid region resembled GII.7/Osaka F140/JPN strain (98%); the strain V1656 showed 98% identity with RdRp region of GII.4/Monastir 375/TUN but capsid region resembled GII.8/Leverkusen 267/DE (91%); the strain V1737 showed 88% identity with RdRp of GII.5/Minato 6/N1/6/JPN whereas capsid region resembled the GII.12/Gifu 96/JPN (93%). During characterization of Caliciviruses two strains of NoV GII.b and one strain of each NoV GI.1/V1622/06/IND, GI.3/V1707/07/IND, GII.3/V1668/IND, GII.16/V1729/IND, Sapovirus GII.1/V1716/IND were also detected.

CONCLUSIONS

The emergence of new variant of GII.4/2007, three novel NoV GII inter-genotype recombinant strains and various other NoVs, indicates the remarkable genetic diversity of the HuCVs as diarrhoeagenic viruses circulating in Kolkata, India.

Authors+Show Affiliations

Molecular Virology Laboratory, Diarrhoeal Disease Research and Control Centre, Division of Virology, National Institute of Cholera & Enteric Diseases, Kolkata, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

19464942

Citation

Nayak, Mukti Kant, et al. "A New Variant of Norovirus GII.4/2007 and Inter-genotype Recombinant Strains of NVGII Causing Acute Watery Diarrhoea Among Children in Kolkata, India." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 45, no. 3, 2009, pp. 223-9.
Nayak MK, Chatterjee D, Nataraju SM, et al. A new variant of Norovirus GII.4/2007 and inter-genotype recombinant strains of NVGII causing acute watery diarrhoea among children in Kolkata, India. J Clin Virol. 2009;45(3):223-9.
Nayak, M. K., Chatterjee, D., Nataraju, S. M., Pativada, M., Mitra, U., Chatterjee, M. K., Saha, T. K., Sarkar, U., & Krishnan, T. (2009). A new variant of Norovirus GII.4/2007 and inter-genotype recombinant strains of NVGII causing acute watery diarrhoea among children in Kolkata, India. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 45(3), 223-9. https://doi.org/10.1016/j.jcv.2009.04.018
Nayak MK, et al. A New Variant of Norovirus GII.4/2007 and Inter-genotype Recombinant Strains of NVGII Causing Acute Watery Diarrhoea Among Children in Kolkata, India. J Clin Virol. 2009;45(3):223-9. PubMed PMID: 19464942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new variant of Norovirus GII.4/2007 and inter-genotype recombinant strains of NVGII causing acute watery diarrhoea among children in Kolkata, India. AU - Nayak,Mukti Kant, AU - Chatterjee,Debarati, AU - Nataraju,S M, AU - Pativada,Madhusudan, AU - Mitra,Utpala, AU - Chatterjee,Mrinal Kanti, AU - Saha,Tushar K, AU - Sarkar,U, AU - Krishnan,Triveni, Y1 - 2009/05/22/ PY - 2009/01/20/received PY - 2009/04/18/revised PY - 2009/04/26/accepted PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/8/12/medline SP - 223 EP - 9 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 45 IS - 3 N2 - BACKGROUND: Noroviruses (NoVs) are one of the major causal agents of acute gastroenteritis among different age groups. Some of the recent studies reveal that NoV genome is highly prone to mutation and recombination which often leads to emergence of new strains. OBJECTIVES: To explore the genetic diversity of human Caliciviruses (HuCVs) among diarrhoeic children in Kolkata. STUDY DESIGN: The HuCVs were detected by reverse transcription-polymerase chain reaction (RT-PCR) of the partial RNA dependent RNA polymerase gene (RdRp) and capsid gene and confirmed by sequencing. The sequences were analyzed and the recombination point was detected. RESULTS: Faecal specimens of children (n=111) visiting outpatient department of Dr B. C. Roy Memorial Hospital for Children with acute gastroenteritis were studied: 22 cases were HuCV positive with 21 NoVs. Of these, 12 NoV cases (54.5%) were GII.4 and six cases showed 99% identity with the new variant Japanese strain Hu/NoV/GII.4/OC07138/JP. Three novel NoV GII inter-genotype recombinant strains V1628/IND, V1656/IND and V1737/IND were also detected. The RdRp region of V1628 showed 96% identity with Pont de Roide 673/FRN whereas capsid region resembled GII.7/Osaka F140/JPN strain (98%); the strain V1656 showed 98% identity with RdRp region of GII.4/Monastir 375/TUN but capsid region resembled GII.8/Leverkusen 267/DE (91%); the strain V1737 showed 88% identity with RdRp of GII.5/Minato 6/N1/6/JPN whereas capsid region resembled the GII.12/Gifu 96/JPN (93%). During characterization of Caliciviruses two strains of NoV GII.b and one strain of each NoV GI.1/V1622/06/IND, GI.3/V1707/07/IND, GII.3/V1668/IND, GII.16/V1729/IND, Sapovirus GII.1/V1716/IND were also detected. CONCLUSIONS: The emergence of new variant of GII.4/2007, three novel NoV GII inter-genotype recombinant strains and various other NoVs, indicates the remarkable genetic diversity of the HuCVs as diarrhoeagenic viruses circulating in Kolkata, India. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/19464942/A_new_variant_of_Norovirus_GII_4/2007_and_inter_genotype_recombinant_strains_of_NVGII_causing_acute_watery_diarrhoea_among_children_in_Kolkata_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(09)00181-4 DB - PRIME DP - Unbound Medicine ER -