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Protection of rat chromosomes by melatonin against gamma radiation-induced damage.

Abstract

The aim of this study was to investigate the protective effect of melatonin (2.5mg/kg/day, given to rats five times by intra-peritoneal injection) against damage in bone-marrow chromosomes induced by a single dose of gamma radiation (4.0 Gy whole-body irradiation; WBI). Ninety-six male albino rats were divided into four equal groups of 24 rats each. They were designated as I-non-irradiated, non-treated control rats, II-non-irradiated rats treated with melatonin for five successive days, III-whole-body gamma-irradiated rats and IV-rats injected with melatonin daily for five successive days, then subjected to whole-body gamma irradiation 2h after the final melatonin injection. Six rats from each group were sacrificed at days 1, 3, 7 and 10 following treatment and/or irradiation and their bone marrows were flushed out for micronuclei scoring and chromosomal analysis. WBI resulted in significant elevations in bone-marrow polychromatic erythrocytes containing micronuclei in their cytoplasm, and caused a significant decrease in the mitotic index of bone-marrow cells; in addition, there was a significant increase in the frequency of aberrant bone-marrow cells and in the different types of structural chromosomal aberration. Melatonin injection prior to WBI significantly reduced the mean frequencies of micro-nucleated polychromatic erythrocytes and of aberrant cells, as well as the incidence of structural chromosomal aberrations in bone-marrow cells; it also caused a highly significant elevation in the value of the mitotic index of bone-marrow cells. This investigation clarifies the protective and/or ameliorative role played by melatonin against deleterious effects of gamma radiation.

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  • Authors

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    Source

    Mutation research 677:1-2 pg 14-20

    MeSH

    Animals
    Antimutagenic Agents
    Bone Marrow Cells
    Chromosome Aberrations
    Chromosomes
    DNA Damage
    Gamma Rays
    Male
    Melatonin
    Micronucleus Tests
    Mutagenicity Tests
    Radiation-Protective Agents
    Rats
    Rats, Wistar
    Whole-Body Irradiation

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19465147

    Citation

    TY - JOUR T1 - Protection of rat chromosomes by melatonin against gamma radiation-induced damage. AU - Assayed,Mohammad E, AU - Abd El-Aty,A M, Y1 - 2009/05/22/ PY - 2009/1/21/received PY - 2009/3/2/revised PY - 2009/4/4/accepted PY - 2009/5/22/aheadofprint PY - 2009/5/26/entrez PY - 2009/5/26/pubmed PY - 2009/9/29/medline SP - 14 EP - 20 JF - Mutation research JO - Mutat. Res. VL - 677 IS - 1-2 N2 - The aim of this study was to investigate the protective effect of melatonin (2.5mg/kg/day, given to rats five times by intra-peritoneal injection) against damage in bone-marrow chromosomes induced by a single dose of gamma radiation (4.0 Gy whole-body irradiation; WBI). Ninety-six male albino rats were divided into four equal groups of 24 rats each. They were designated as I-non-irradiated, non-treated control rats, II-non-irradiated rats treated with melatonin for five successive days, III-whole-body gamma-irradiated rats and IV-rats injected with melatonin daily for five successive days, then subjected to whole-body gamma irradiation 2h after the final melatonin injection. Six rats from each group were sacrificed at days 1, 3, 7 and 10 following treatment and/or irradiation and their bone marrows were flushed out for micronuclei scoring and chromosomal analysis. WBI resulted in significant elevations in bone-marrow polychromatic erythrocytes containing micronuclei in their cytoplasm, and caused a significant decrease in the mitotic index of bone-marrow cells; in addition, there was a significant increase in the frequency of aberrant bone-marrow cells and in the different types of structural chromosomal aberration. Melatonin injection prior to WBI significantly reduced the mean frequencies of micro-nucleated polychromatic erythrocytes and of aberrant cells, as well as the incidence of structural chromosomal aberrations in bone-marrow cells; it also caused a highly significant elevation in the value of the mitotic index of bone-marrow cells. This investigation clarifies the protective and/or ameliorative role played by melatonin against deleterious effects of gamma radiation. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/19465147/Protection_of_rat_chromosomes_by_melatonin_against_gamma_radiation_induced_damage_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S1383-5718(09)00170-3 ER -